Rabbit Polyclonal E2F1 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human E2F1 aa 1-250.
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- Respiratory research 23:3402022Involvement of miRNA-34a regulated Krüppel-like factor 4 expression in hyperoxia-induced senescence in lung epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHajime Maeda,Hongwei Yao,Hayato Go,Kelsey E Huntington,Monique E De Paepe,Phyllis A DenneryPubMed 36496404
- Frontiers in oncology 12:10364012022DJ-1 promotes osteosarcoma progression through activating CDK4/RB/E2F1 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhitao Han,Lining Wang,Dongshuo Wang,Luosheng Zhang,Yifeng Bi,Xinlei Zheng,Weibo Liu,Guangjian Bai,Zhenhua Wang,Wei Wan,Yong Ma,Xiaopan Cai,Tielong Liu,Qi JiaPubMed 36408174
- World journal of surgical oncology 20:882022Positive regulation of ataxia-telangiectasia-mutated protein (ATM) by E2F transcription Factor 1 (E2F-1) in cisplatin-resistant nasopharyngeal carcinoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZun-Yan Zhou,Ji-Yuan Yang,Cheng-Ze Shao,Fei Luo,Wei DuPubMed 35303867
- Oncology letters 22:6602021MicroRNA-1298-5p inhibits the tumorigenesis of breast cancer by targeting E2F1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Zhang,Chenyang Hu,Dawei Hu,Zhimin FanPubMed 34386082
- Frontiers in cell and developmental biology 8:5281552020Hsa_circ_0088233 Alleviates Proliferation, Migration, and Invasion of Prostate Cancer by Targeting hsa-miR-185-3p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhi-Hai Deng et. al.PubMed 33195183
- EMBO molecular medicine 12:e106222020HDAC1 dysregulation induces aberrant cell cycle and DNA damage in progress of TDP-43 proteinopathies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng-Chun Wu et. al.PubMed 32449313
- Journal of cell science 129:693-7052016Loss of PPARγ in endothelial cells leads to impaired angiogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSanna Vattulainen-Collanus et. al.PubMed 26743080
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human E2F1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q01094
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Predicted |
| Cow | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
Target data
Function
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication (PubMed:10675335, PubMed:12717439, PubMed:17050006, PubMed:17704056, PubMed:18625225, PubMed:28992046). The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase (PubMed:10675335, PubMed:12717439, PubMed:17704056). E2F1 binds preferentially RB1 in a cell-cycle dependent manner (PubMed:10675335, PubMed:12717439, PubMed:17704056). It can mediate both cell proliferation and TP53/p53-dependent apoptosis (PubMed:8170954). Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812). Directly activates transcription of PEG10 (PubMed:17050006, PubMed:18625225, PubMed:28992046). Positively regulates transcription of RRP1B (PubMed:20040599).
Alternative names
RBBP3, E2F1, Transcription factor E2F1, E2F-1, PBR3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, pRB-binding protein E2F-1, RBAP-1, RBBP-3
Recommended products
Rabbit Polyclonal E2F1 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human E2F1 aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human E2F1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q01094
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The E2F1 protein also known as E2F transcription factor 1 is an important regulator of the cell cycle. It has a molecular weight of approximately 47 kDa. E2F1 protein is expressed in many tissues with higher expression levels in proliferating cells. It often acts as a transcription factor by binding to specific DNA sequences controlling the transition of cells from G1 to S phase of the cell cycle. This role is vital for the proper progression of the cycle and cellular proliferation.
- Biological function summary
E2F1 plays a significant role in cell growth regulation and apoptosis. E2F1 functions within a complex often forming heterodimers with DP proteins. This combination enhances its ability to bind DNA and activate transcription of genes necessary for DNA replication and cell cycle progression. E2F1 also participates in the control of apoptosis by regulating the expression of pro-apoptotic genes balancing cell proliferation and death.
- Pathways
E2F1 is deeply embedded in cell cycle regulatory pathways and the p53 signaling pathway. It closely interacts with proteins like RB (Retinoblastoma protein) and p53. RB protein regulates E2F1 activity by controlling its release while p53 helps in mediating the cell's response to DNA damage potentially leading to either cell cycle arrest or apoptosis. These interactions highlight E2F1's role in maintaining cellular homeostasis.
- Associated diseases and disorders
E2F1 is often linked to cancer and neurodegenerative diseases. Overexpression or dysregulation of E2F1 can lead to uncontrolled cell proliferation contributing to oncogenesis. In the context of neurodegenerative diseases E2F1 influences neuronal apoptosis and may be associated with disorders such as Alzheimer's. E2F1 interacts with other proteins such as p53 in cancer where it affects both cell cycle dynamics and apoptosis illustrating its complex involvement in disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-E2F1 antibody (ab137415)
10% SDS PAGE
All lanes: Western blot - Anti-E2F1 antibody (ab137415) at 1/500 dilution
All lanes: A431 whole cell lysate at 30 µg
Predicted band size: 47 kDa
Western blot - Anti-E2F1 antibody (ab137415)
10% SDS PAGE
All lanes: Western blot - Anti-E2F1 antibody (ab137415) at 1/3000 dilution
All lanes: NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 47 kDa
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-E2F1 antibody (ab137415)
E2F1 western blot using anti-E2F1 antibody ab137415. Publication image and figure legend from Wu, C. C., Jin, L. W., et al., 2020, EMBO Mol Med, PubMed 32449313.
ab137415 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab137415 please see the product overview.
Aberrant cell cycle activity correlates with TDP‐43 proteinopathies in FTLD‐TDP Tg miceARepresentative immunofluorescence (IF) staining of Ki67 and TDP‐43 in the regions of frontal cortices from 6‐mon‐old WT and FTLD‐TDP Tg mice. Nuclei were stained with 4′,6‐diamidino‐2‐phenylindole (DAPI; upper panel in blue) or neural marker NeuN (lower panel in green). Scale bar: 50 μm. The circled area is emphasized for showing the distribution of immunoreactivity in cell subregions. Scale bar: 15 μm.BQuantification of cells or neurons with Ki67 immunoreactivity and TDP‐43 mislocalization from each view of microscope. n = 9 sections per mouse, N = 5 mice per group, data are presented as mean ± SEM, ***P = 0.0007 by t‐test.CRepresentative data of reverse transcription PCR (left panel) or Western blot (right panel) for cell cycle‐related genes and semi‐quantification of the expression levels in the frontal cortices and hippocampus from the 6‐mon‐old WT and FTLD‐TDP Tg mice. N = 5 mice per group, data are presented as mean ± SEM (%), statistical analysis by multiple t‐test with FDR correction, Q = 1%. *P < 0.05, **P ≤ 0.01; ***P ≤ 0.001 and ****P ≤ 0.0001, exact P values are shown in Appendix Table S1.Source data are available online for this figure.
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