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AB5391

Anti-E2F1 (phospho S364) antibody

4

(1 Review)

|

(7 Publications)

Rabbit Polyclonal E2F1 phospho S364 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human E2F1 phospho S364 aa 350-400 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

RBBP3, E2F1, Transcription factor E2F1, E2F-1, PBR3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, pRB-binding protein E2F-1, RBAP-1, RBBP-3

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 (phospho S364) antibody (AB5391)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 (phospho S364) antibody (AB5391)

ab5391 at 10 μg/ml staining human breast carcinoma.

Western blot - Anti-E2F1 (phospho S364) antibody (AB5391)
  • WB

Unknown

Western blot - Anti-E2F1 (phospho S364) antibody (AB5391)

Lysates were prepared from untreated cells and cells treated with 2μM Doxorubicin used as a DNA damaging agent. The arrow indicates E2F1.

Phosphorylated E2F1 was only expressed in the treated cells. The blot clearly shows that ab5391 only detected phosphorylated E2F1 in the human cell lines.

All lanes:

Western blot - Anti-E2F1 (phospho S364) antibody (ab5391) at 1/250 dilution

Lane 1:

CRE (mouse) cells transfected with human E2F1

Lane 2:

CRE (mouse) cells transfected with human E2F1 treated with 2μM Doxorubicin

Lane 3:

NiH3T3 (mouse) cell lysate

Lane 4:

NiH3T3 (mouse) cell lysate treated with 2μM Doxorubicin

Lane 5:

MDA-231 (human breast cancer) cell lysate

Lane 6:

MDA-231 (human breast cancer) cell lysate treated with 2μM Doxorubicin

Secondary

All lanes:

HRP conjugated Goat-anti-Rabbit IgG MX10

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human E2F1 phospho S364 aa 350-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q01094

Specificity

This antibody is specific for the phosphorylated form of E2F1. Minimal cross-reactivity is seen with non-phosphorylated human E2F1 by peptide ELISA.

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Primary Antibodies

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Primary Antibodies

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Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

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Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

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  • 230
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This affinity purified antibody is directed against the phosphorylated form of human E2F-1 at the pS364 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The E2F1 protein also known as E2F transcription factor 1 is an important regulator of the cell cycle. It has a molecular weight of approximately 47 kDa. E2F1 protein is expressed in many tissues with higher expression levels in proliferating cells. It often acts as a transcription factor by binding to specific DNA sequences controlling the transition of cells from G1 to S phase of the cell cycle. This role is vital for the proper progression of the cycle and cellular proliferation.
Biological function summary

E2F1 plays a significant role in cell growth regulation and apoptosis. E2F1 functions within a complex often forming heterodimers with DP proteins. This combination enhances its ability to bind DNA and activate transcription of genes necessary for DNA replication and cell cycle progression. E2F1 also participates in the control of apoptosis by regulating the expression of pro-apoptotic genes balancing cell proliferation and death.

Pathways

E2F1 is deeply embedded in cell cycle regulatory pathways and the p53 signaling pathway. It closely interacts with proteins like RB (Retinoblastoma protein) and p53. RB protein regulates E2F1 activity by controlling its release while p53 helps in mediating the cell's response to DNA damage potentially leading to either cell cycle arrest or apoptosis. These interactions highlight E2F1's role in maintaining cellular homeostasis.

E2F1 is often linked to cancer and neurodegenerative diseases. Overexpression or dysregulation of E2F1 can lead to uncontrolled cell proliferation contributing to oncogenesis. In the context of neurodegenerative diseases E2F1 influences neuronal apoptosis and may be associated with disorders such as Alzheimer's. E2F1 interacts with other proteins such as p53 in cancer where it affects both cell cycle dynamics and apoptosis illustrating its complex involvement in disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication (PubMed : 10675335, PubMed : 12717439, PubMed : 17050006, PubMed : 17704056, PubMed : 18625225, PubMed : 28992046). The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase (PubMed : 10675335, PubMed : 12717439, PubMed : 17704056). E2F1 binds preferentially RB1 in a cell-cycle dependent manner (PubMed : 10675335, PubMed : 12717439, PubMed : 17704056). It can mediate both cell proliferation and TP53/p53-dependent apoptosis (PubMed : 8170954). Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed : 20176812). Directly activates transcription of PEG10 (PubMed : 17050006, PubMed : 18625225, PubMed : 28992046). Positively regulates transcription of RRP1B (PubMed : 20040599).
See full target information E2F1 phospho S364
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Aging 15:4182-4201 PubMed37199665

2023

Sig1R activates extracellular matrix-induced bladder cancer cell proliferation and angiogenesis by combing β-integrin.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Zhao,Tianli Yang,Liuhua Zhou,Rongfei Li,Jingyu Liu,Jun Zhao,Ruipeng Jia

Journal of hematology & oncology 15:168 PubMed36434634

2022

Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yexin Tong,Mingjun Sun,Lingli Chen,Yunzhi Wang,Yan Li,Lingling Li,Xuan Zhang,Yumeng Cai,Jingbo Qie,Yanrui Pang,Ziyan Xu,Jiangyan Zhao,Xiaolei Zhang,Yang Liu,Sha Tian,Zhaoyu Qin,Jinwen Feng,Fan Zhang,Jiajun Zhu,Yifan Xu,Wenhui Lou,Yuan Ji,Jianyuan Zhao,Fuchu He,Yingyong Hou,Chen Ding

Journal of cellular physiology 236:4050-4065 PubMed33174204

2020

GBE attenuates arsenite-induced hepatotoxicity by regulating E2F1-autophagy-E2F7a pathway and restoring lysosomal activity.

Applications

Unspecified application

Species

Unspecified reactive species

Xiong Chen,Dapeng Wang,Baofei Sun,Chunyan Liu,Kai Zhu,Aihua Zhang

Endocrine-related cancer 23:857-870 PubMed27935805

2016

E2F1-mediated human POMC expression in ectopic Cushing's syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Takako Araki,Ning-Ai Liu,Yukiko Tone,Daniel Cuevas-Ramos,Roy Heltsley,Masahide Tone,Shlomo Melmed

Cancer discovery 3:182-97 PubMed23306062

2013

Senescence sensitivity of breast cancer cells is defined by positive feedback loop between CIP2A and E2F1.

Applications

Unspecified application

Species

Human

Anni Laine,Harri Sihto,Christophe Come,Mathias T Rosenfeldt,Aleksandra Zwolinska,Minna Niemelä,Anchit Khanna,Edward K Chan,Veli-Matti Kähäri,Pirkko-Liisa Kellokumpu-Lehtinen,Owen J Sansom,Gerard I Evan,Melissa R Junttila,Kevin M Ryan,Jean-Christophe Marine,Heikki Joensuu,Jukka Westermarck

Molecular cancer research : MCR 10:1189-202 PubMed22802261

2012

The p38 MAPK-MK2 axis regulates E2F1 and FOXM1 expression after epirubicin treatment.

Applications

Unspecified application

Species

Human

Natalia de Olano,Chuay-Yeng Koo,Lara J Monteiro,Paola H Pinto,Ana R Gomes,Rosa Aligue,Eric W-F Lam

Molecular and cellular biology 29:68-82 PubMed18955500

2008

Death receptor-induced activation of the Chk2- and histone H2AX-associated DNA damage response pathways.

Applications

WB

Species

Human

Stéphanie Solier,Olivier Sordet,Kurt W Kohn,Yves Pommier
View all publications
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Product promise

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