AB55325

Anti-E2F1 (phospho T433) antibody

Name: Anti-E2F1 (phospho T433) antibody (ab55325) | Abcam
Brand: Abcam Limited
SKU: AB55325
Price: 535 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(5 Publications)

Rabbit Polyclonal E2F1 phospho T433 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human E2F1 phospho T433 aa 350 to C-terminus.

View Alternative Names

RBBP3, E2F1, Transcription factor E2F1, E2F-1, PBR3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, pRB-binding protein E2F-1, RBAP-1, RBBP-3

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 (phospho T433) antibody (AB55325)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 (phospho T433) antibody (AB55325)

ab55325 staining human normal pancreas. Staining is localised to nuclear compartment.
Left panel : with primary antibody at 4 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH6 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

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Western blot - Anti-E2F1 (phospho T433) antibody (AB55325)

All lanes:

Western blot - Anti-E2F1 (phospho T433) antibody (ab55325) at 1/500 dilution

Lane 1:

HeLa cell extract treated with Etoposide (at 25µM for 24 hrs).

Lane 2:

HeLa cell extract treated with Etoposide (at 25µM for 24 hrs), and with the immunising phosphopeptide.

Predicted band size: 47 kDa

Observed band size: 47 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human E2F1 phospho T433 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q01094

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The E2F1 protein also known as E2F transcription factor 1 is an important regulator of the cell cycle. It has a molecular weight of approximately 47 kDa. E2F1 protein is expressed in many tissues with higher expression levels in proliferating cells. It often acts as a transcription factor by binding to specific DNA sequences controlling the transition of cells from G1 to S phase of the cell cycle. This role is vital for the proper progression of the cycle and cellular proliferation.

Biological function summary

E2F1 plays a significant role in cell growth regulation and apoptosis. E2F1 functions within a complex often forming heterodimers with DP proteins. This combination enhances its ability to bind DNA and activate transcription of genes necessary for DNA replication and cell cycle progression. E2F1 also participates in the control of apoptosis by regulating the expression of pro-apoptotic genes balancing cell proliferation and death.

Pathways

E2F1 is deeply embedded in cell cycle regulatory pathways and the p53 signaling pathway. It closely interacts with proteins like RB (Retinoblastoma protein) and p53. RB protein regulates E2F1 activity by controlling its release while p53 helps in mediating the cell's response to DNA damage potentially leading to either cell cycle arrest or apoptosis. These interactions highlight E2F1's role in maintaining cellular homeostasis.

E2F1 is often linked to cancer and neurodegenerative diseases. Overexpression or dysregulation of E2F1 can lead to uncontrolled cell proliferation contributing to oncogenesis. In the context of neurodegenerative diseases E2F1 influences neuronal apoptosis and may be associated with disorders such as Alzheimer's. E2F1 interacts with other proteins such as p53 in cancer where it affects both cell cycle dynamics and apoptosis illustrating its complex involvement in disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication (PubMed : 10675335, PubMed : 12717439, PubMed : 17050006, PubMed : 17704056, PubMed : 18625225, PubMed : 28992046). The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase (PubMed : 10675335, PubMed : 12717439, PubMed : 17704056). E2F1 binds preferentially RB1 in a cell-cycle dependent manner (PubMed : 10675335, PubMed : 12717439, PubMed : 17704056). It can mediate both cell proliferation and TP53/p53-dependent apoptosis (PubMed : 8170954). Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed : 20176812). Directly activates transcription of PEG10 (PubMed : 17050006, PubMed : 18625225, PubMed : 28992046). Positively regulates transcription of RRP1B (PubMed : 20040599).

See full target information E2F1 phospho T433

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 236:4050-4065 PubMed33174204

2020

GBE attenuates arsenite-induced hepatotoxicity by regulating E2F1-autophagy-E2F7a pathway and restoring lysosomal activity.

Applications

Unspecified application

Species

Unspecified reactive species

Xiong Chen,Dapeng Wang,Baofei Sun,Chunyan Liu,Kai Zhu,Aihua Zhang

Biochimica et biophysica acta. Molecular basis of 1865:165523 PubMed31376482

2019

IDH2 deficiency impairs cutaneous wound healing via ROS-dependent apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sung Hwan Kim,Jeen-Woo Park

Gene 686:1-7 PubMed30366080

2018

Long noncoding RNA DLX6-AS1 accelerates the glioma carcinogenesis by competing endogenous sponging miR-197-5p to relieve E2F1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangpan Li,Huibo Zhang,Xiaofei Wu

The EMBO journal 36:2353-2372 PubMed28701484

2017

SIRT1 regulates macrophage self-renewal.

Applications

Unspecified application

Species

Unspecified reactive species

Francesco Imperatore,Julien Maurizio,Stephanie Vargas Aguilar,Clara J Busch,Jérémy Favret,Elisabeth Kowenz-Leutz,Wilfried Cathou,Rebecca Gentek,Pierre Perrin,Achim Leutz,Carole Berruyer,Michael H Sieweke

BMC genomics 14:525 PubMed23902294

2013

TGFβ and CCN2/CTGF mediate actin related gene expression by differential E2F1/CREB activation.

Applications

Species

Rat

Noel Faherty,Helen O'Donovan,David Kavanagh,Stephen Madden,Gareth J McKay,Alexander P Maxwell,Finian Martin,Catherine Godson,John Crean

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-E2F1 (phospho T433) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 (phospho T433) antibody (AB55325)

Western blot - Anti-E2F1 (phospho T433) antibody (AB55325)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise