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AB318957

Anti-E2F2 antibody [EPR27391-170] - BSA and Azide free

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Rabbit Recombinant Monoclonal E2F2 antibody. Carrier free. Suitable for WB, IP, ChIP-seq and reacts with Recombinant full length protein - Human, Human samples.

View Alternative Names

Transcription factor E2F2, E2F-2, E2F2

8 Images
ChIP-sequencing - Anti-E2F2 antibody [EPR27391-170] - BSA and Azide free (AB318957)
  • ChIP-seq

Supplier Data

ChIP-sequencing - Anti-E2F2 antibody [EPR27391-170] - BSA and Azide free (AB318957)

This data was developed using ab318956, the same antibody clone in a different buffer formulation.

Chromatin was prepared from Raji cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 8 µg of ab318956 [EPR27391-170]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 60 million reads. The Input control is also shown.

ChIP-sequencing - Anti-E2F2 antibody [EPR27391-170] - BSA and Azide free (AB318957)
  • ChIP-seq

Supplier Data

ChIP-sequencing - Anti-E2F2 antibody [EPR27391-170] - BSA and Azide free (AB318957)

This data was developed using ab318956, the same antibody clone in a different buffer formulation.

Chromatin was prepared from Raji cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 8 µg of ab318956 [EPR27391-170]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 60 million reads. The Input control is also shown.

Immunoprecipitation - Anti-E2F2 antibody [EPR27391-170] - BSA and Azide free (AB318957)
  • IP

Supplier Data

Immunoprecipitation - Anti-E2F2 antibody [EPR27391-170] - BSA and Azide free (AB318957)

This data was developed using ab318956, the same antibody clone in a different buffer formulation.

E2F2 was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate with ab318956 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab318956 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : ab318956 IP in Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab318956 in Raji whole cell lysate

All lanes:

Immunoprecipitation - Anti-E2F2 antibody [EPR27391-170] (<a href='/en-us/products/primary-antibodies/e2f2-antibody-epr27391-170-ab318956'>ab318956</a>) at 1/30 dilution

All lanes:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate with NFDM/TBST

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

Immunoprecipitation - Anti-E2F2 antibody [EPR27391-170] - BSA and Azide free (AB318957)
  • IP

Supplier Data

Immunoprecipitation - Anti-E2F2 antibody [EPR27391-170] - BSA and Azide free (AB318957)

This data was developed using ab318956, the same antibody clone in a different buffer formulation.

E2F2 was immunoprecipitated from 0.35 mg Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate with ab318956 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab318956 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : ab318956 IP in Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab318956 in Ramos whole cell lysate

All lanes:

Immunoprecipitation - Anti-E2F2 antibody [EPR27391-170] (<a href='/en-us/products/primary-antibodies/e2f2-antibody-epr27391-170-ab318956'>ab318956</a>) at 1/30 dilution

All lanes:

Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate with NFDM/TBST

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

ChIP-sequencing - Anti-E2F2 antibody [EPR27391-170] - BSA and Azide free (AB318957)
  • ChIP-seq

Supplier Data

ChIP-sequencing - Anti-E2F2 antibody [EPR27391-170] - BSA and Azide free (AB318957)

This data was developed using ab318956, the same antibody clone in a different buffer formulation.

Chromatin was prepared from Raji cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 8 µg of ab318956 [EPR27391-170]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 60 million reads. The Input control is also shown.

Western blot - Anti-E2F2 antibody [EPR27391-170] - BSA and Azide free (AB318957)
  • WB

Supplier Data

Western blot - Anti-E2F2 antibody [EPR27391-170] - BSA and Azide free (AB318957)

This data was developed using ab318956, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-E2F2 antibody [EPR27391-170] (<a href='/en-us/products/primary-antibodies/e2f2-antibody-epr27391-170-ab318956'>ab318956</a>) at 1/1000 dilution

Lane 1:

Human lung cancer tissue lysate at 50 µg with NFDM/TBST

Lane 2:

Human liver cancer tissue lysate at 50 µg with NFDM/TBST

Lane 3:

Human placenta tissue lysate at 50 µg with NFDM/TBST

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 74 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-E2F2 antibody [EPR27391-170] - BSA and Azide free (AB318957)
  • WB

Supplier Data

Western blot - Anti-E2F2 antibody [EPR27391-170] - BSA and Azide free (AB318957)

This data was developed using ab318956, the same antibody clone in a different buffer formulation.

Low expression : K-562, 293T.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

The high-sensitivity ECL substrate allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-E2F2 antibody [EPR27391-170] (<a href='/en-us/products/primary-antibodies/e2f2-antibody-epr27391-170-ab318956'>ab318956</a>) at 1/1000 dilution

Lane 1:

Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 50 µg with NFDM/TBST

Lane 2:

Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate at 50 µg with NFDM/TBST

Lane 3:

Ramos (human Burkitts lymphoma B lymphocyte) whole cell lysate at 50 µg with NFDM/TBST

Lane 4:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 50 µg with NFDM/TBST

Lane 5:

293T (human embryonic kidney epithelial cell) whole cell lysate at 50 µg with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 74 kDa,36 kDa

true

Exposure time: 180s

Western blot - Anti-E2F2 antibody [EPR27391-170] - BSA and Azide free (AB318957)
  • WB

Supplier Data

Western blot - Anti-E2F2 antibody [EPR27391-170] - BSA and Azide free (AB318957)

This data was developed using ab318956, the same antibody clone in a different buffer formulation.

This antibody does not cross-react with human E2F3.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-E2F2 antibody [EPR27391-170] (<a href='/en-us/products/primary-antibodies/e2f2-antibody-epr27391-170-ab318956'>ab318956</a>) at 1/1000 dilution

Lane 1:

His-tagged human E2F2 recombinant protein at 10 ng with NFDM/TBST

Lane 2:

His-tagged human E2F3 recombinant protein at 10 ng with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 74 kDa

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27391-170

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ChIP-seq, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ChIPseq-species-checked": "testedAndGuaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ChIPseq-species-checked": "notRecommended", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIPseq-species-checked": "notRecommended", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIPseq-species-checked": "notRecommended", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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AB185913

High-Sensitivity ChIP Kit

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

ab318957 is the carrier-free version of ab318956.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

E2F2 also known as E2F transcription factor 2 is a member of the E2F family of transcription factors. It is a protein with a mass of approximately 46 kDa. It plays an important role by regulating the expression of genes involved in cell cycle progression particularly the transition from G1 to S phase. Researchers have identified that E2F2 is expressed in various tissues with high expression noted in actively dividing cells such as those in fetal tissues adult testis and tumor cells.
Biological function summary

E2F2 functions as a transcriptional activator and repressor controlling genes necessary for DNA synthesis and cell cycle progression. It often associates with retinoblastoma protein (RB1) to form complexes that finely regulate gene transcription. E2F2 not only controls cell proliferation but also influences cell differentiation processes by coordinating the balance between distinct cellular states.

Pathways

E2F2 interacts with key players in the cell cycle such as cyclins and cyclin-dependent kinases (CDKs). It is an integral part of the Rb-E2F signaling pathway which governs cell cycle checkpoints and progression. This pathway's regulation heavily influences the activities of other E2F family members such as E2F1 and E2F3 which can either promote or inhibit cell cycle transitions in response to various signals.

E2F2's dysregulation associates with cancer and retinoblastoma. Uncontrolled E2F2 activity often contributes to tumorigenesis by bypassing cell cycle controls resulting in unchecked cell division. In cancer altered interactions between E2F2 and proteins like RB1 can lead to the loss of tumor suppressor functions such as those observed in certain aggressive tumors. Understanding these interactions can provide insights into therapeutic targets for cancer treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.
See full target information E2F2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com