Anti-E2F3 antibody

• WB

Lab

Western blot - Anti-E2F3 antibody (AB152126)

False colour image of Western blot : Anti-E2F3 antibody staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab152126 was shown to bind specifically to E2F3. A band was observed at 49 kDa in wild-type HeLa cell lysates with no signal observed at this size in E2F3 knockout cell line ab265362 (knockout cell lysate ab257415). To generate this image, wild-type and E2F3 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-E2F3 antibody (ab152126) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

E2F3 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human E2F3 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-e2f3-knockout-hela-cell-line-ab265362'>ab265362</a>)

Lane 3:

SH-SY5Y cell lysate at 20 µg

Lane 4:

U-87 MG cell lysate at 20 µg

Predicted band size: 49 kDa

Observed band size: 49 kDa

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• WB

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Western blot - Anti-E2F3 antibody (AB152126)

10% SDS PAGE

All lanes:

Western blot - Anti-E2F3 antibody (ab152126) at 1/1000 dilution

All lanes:

293T whole cell lysate at 30 µg

Predicted band size: 49 kDa

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