Rabbit Polyclonal E2F3 antibody. Suitable for WB and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human E2F3 aa 150-450.
Images
Publications
- Journal of Cancer 14:1362-13702023MiR-363 restrain the proliferation, migration and invasion of colorectal carcinoma cell by targeting E2F3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenyu Yang,Xiqian Yang,Yang Zhang,Yunhai Li,Wenliang LvPubMed 37283793
- Aging 15:2689-27042023Long noncoding RNA POU6F2-AS2 contributes to the aggressiveness of nonsmall-cell lung cancer via microRNA-125b-5p-mediated E2F3 upregulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaitao Yang,Xiao Feng,Xiangdong TongPubMed 37053020
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human E2F3 aa 150-450. The exact immunogen used to generate this antibody is proprietary information. Database link O00716
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Cow | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow | Dilution info - | Notes - |
Target data
Function
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters (By similarity).
Alternative names
Transcription factor E2F3, E2F-3, E2F3, KIAA0075
Recommended products
Rabbit Polyclonal E2F3 antibody. Suitable for WB and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human E2F3 aa 150-450.
Alternative names
Transcription factor E2F3, E2F-3, E2F3, KIAA0075
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human E2F3 aa 150-450. The exact immunogen used to generate this antibody is proprietary information. Database link O00716
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- Activity summary
E2F3 also known by its alternate name E2F transcription factor 3 functions as a member of the E2F family of transcription factors which are important for the regulation of the cell cycle. This protein possesses a molecular mass of approximately 55 kDa. E2F3 is expressed in various tissues with significant expression in proliferative cells. It plays a critical role in transcription activation binding with DNA and regulating genes necessary for cell cycle progression especially during the transition from the G1 to the S phase.
- Biological function summary
E2F3 forms part of the E2F/DP protein complex which controls cell cycle entry and progression. Its activity facilitates the transcription of genes essential for DNA replication and synthesis. Through its interaction with retinoblastoma proteins specifically pRB and p107 E2F3 modulates cellular proliferation. This regulation is vital for maintaining normal cellular functions and growth ensuring proper cell cycle checkpoints are upheld.
- Pathways
E2F3 participates in the RB/E2F signaling pathway a major controller of cell proliferation and apoptosis. This pathway serves a critical function in the decision between cell cycle progression and arrest. E2F3 interacts with cyclins and cyclin-dependent kinases (CDKs) particularly CDK2 highlighting its role in the regulation of cell cycle dynamics. It also works alongside other E2F family members such as E2F1 and E2F2 to maintain the balance between cell growth and death.
- Associated diseases and disorders
E2F3 has implications in cancer most notably in bladder cancer due to its ability to drive uncontrolled cell proliferation when regulatory mechanisms fail. Overexpression of E2F3 often correlates with tumorigenesis serving as a potential biomarker for aggressive forms of cancer. The dysregulation of E2F3 in conjunction with pRB pathway alterations has links to retinoblastoma a rare eye cancer. In these contexts E2F3's activity becomes deregulated contributing to disease progression through its interaction with proteins like pRB and CDKs.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-E2F3 antibody (ab152126)
False colour image of Western blot: Anti-E2F3 antibody staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab152126 was shown to bind specifically to E2F3. A band was observed at 49 kDa in wild-type HeLa cell lysates with no signal observed at this size in E2F3 knockout cell line Human E2F3 knockout HeLa cell line ab265362 (knockout cell lysate Human E2F3 knockout HeLa cell lysate ab257415). To generate this image, wild-type and E2F3 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-E2F3 antibody (ab152126) at 1/500 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: E2F3 knockout HeLa cell lysate at 20 µg
Lane 3: SH-SY5Y cell lysate at 20 µg
Lane 4: U-87 MG cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Western blot - Anti-E2F3 antibody (ab152126)
10% SDS PAGE
All lanes: Western blot - Anti-E2F3 antibody (ab152126) at 1/1000 dilution
All lanes: 293T whole cell lysate at 30 µg
Predicted band size: 49 kDa
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