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AB234997

Anti-E2F4 antibody

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(2 Publications)

Rabbit Polyclonal E2F4 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human E2F4 aa 150-300.

View Alternative Names

Transcription factor E2F4, E2F-4, E2F4

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody (AB234997)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody (AB234997)

Paraffin-embedded human lung tissue stained for E2F4 using ab234997 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-E2F4 antibody (AB234997)
  • WB

Supplier Data

Western blot - Anti-E2F4 antibody (AB234997)

All lanes:

Western blot - Anti-E2F4 antibody (ab234997) at 1/500 dilution

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Secondary

All lanes:

Polyclonal Goat anti-rabbit IgG at 1/50000 dilution

Predicted band size: 22 kDa,44 kDa,47 kDa,53 kDa,57 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human E2F4 aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

Q16254

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

E2F4 sometimes known as E2F-4 is part of the E2F family of transcription factors. It plays a critical role in controlling the cell cycle by regulating the expression of genes necessary for DNA synthesis. E2F4 has a molecular weight of around 45 kDa. These transcription factors are widely expressed in a variety of tissues reflecting their importance in cell biology. E2F4 typically functions by forming complexes with dimerization partner (DP) proteins mainly in the nucleus where it binds to DNA and influences transcription.
Biological function summary

E2F4 functions as a regulator of cell cycle progression. It forms complexes localizing mostly in the nucleus controlling the transition from G1 to S phase by regulating genes associated with cell cycle arrest and DNA replication. Partnering with members of the DP family enhances its DNA binding capacity influencing genes involved in cell cycle arrest and DNA repair processes. E2F4 predominantly acts as a transcriptional repressor in quiescent and differentiating cells maintaining the cell in a non-proliferative state.

Pathways

E2F4 is significant in the regulation of the cell cycle and DNA damage response pathways. It engages with the retinoblastoma protein family (pRB p107 p130) to exert repressive effects on E2F-target genes ensuring proper cell cycle exit and maintaining the G0 phase. The interaction with p130 is instrumental during the G0 state preventing inappropriate cell proliferation and contributing to the maintenance of cellular quiescence.

E2F4's role is noteworthy in cancer and retinoblastoma. Alterations in its regulation or activity can lead to unchecked cell proliferation a hallmark of cancerous growths. The malfunction or suppression of its activity can disrupt cell cycle control contributing to oncogenesis. In instances of retinoblastoma improper interaction between E2F4 and the retinoblastoma protein family can result in disrupted cell cycle checkpoints highlighting its importance in maintaining cellular homeostasis and preventing tumorigenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation : together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
See full target information E2F4
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:29643 PubMed40804093

2025

miR-221 activates Sox11 to reduce brain injury after intracerebral hemorrhage via inhibiting neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyu Liang,Yongchun Liu,Renyang Liu,Xianmei Shen,Mingxia Xu

Gene 684:39-46 PubMed30359737

2018

Competitive regulation by transcription factors and DNA methylation in the bovine SIRT5 promoter: Roles of E2F4 and KLF6.

Applications

Unspecified application

Species

Unspecified reactive species

Jieyun Hong,Xiaoyu Wang,Chugang Mei,Linsen Zan
View all publications
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Product promise

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