Anti-E2F4 antibody [EPR8259]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (AB150360)

Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using ab150360 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (AB150360)

Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labelling E2F4 with ab150360 antibody at a dilution of 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (AB150360)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling E2F4 with ab150360 antibody at a dilution of 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (AB150360)

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab150360 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (AB150360)

Immunohistochemical analysis of paraffin embedded Human Thyroid gland papillary carcinoma tissue using ab150360 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (AB150360)

Immunohistochemical analysis of paraffin embedded Normal Human Colon tissue using ab150360 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-E2F4 antibody [EPR8259] (AB150360)

Lanes 1-4 : Merged signal (red and green). Green - ab150360 observed at 60 kDa. Red - loading control ab8245 observed at 36 kDa.

ab150360 Anti-E2F4 antibody [EPR8259] was shown to specifically react with E2F4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266119 (knockout cell lysate ab257932) was used. Wild-type and E2F4 knockout samples were subjected to SDS-PAGE. ab150360 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-E2F4 antibody [EPR8259] (ab150360) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

E2F4 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human E2F4 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-e2f4-knockout-hek-293t-cell-line-ab266119'>ab266119</a>)

Lane 3:

K-562 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 44 kDa

Observed band size: 60 kDa

false

• WB

Unknown

Western blot - Anti-E2F4 antibody [EPR8259] (AB150360)

All lanes:

Western blot - Anti-E2F4 antibody [EPR8259] (ab150360) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

Raji cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 44 kDa

Observed band size: 60 kDa

false

• WB

CiteAb

Western blot - Anti-E2F4 antibody [EPR8259] (AB150360)

E2F4 western blot using anti-E2F4 antibody [EPR8259] ab150360. Publication image and figure legend from Li, L. L., Xue, A. M., et al., 2014, Breast Cancer Res, PubMed 24886710.

ab150360 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab150360 please see the product overview.

E2F and histone deacetylase (HDAC) are involved in the transcriptional inhibition of ARHI by JMJD2A. (A) Specific siRNAs against E2F1 and E2F4 were efficient to knockdown E2F1 and E2F4 expression level. (B) Effects of E2Fs knockdown on JMJD2A binding to the A2 site of the ARHI promoter. Both siRNAs could inhibit the binding of JMJD2A to the ARHI promoter. (C) Effects of JMJD2A-FL, JMJD2A mutants (H188A and M867) and trichostatin A (TSA) on ARHI promoter activity. *p < 0.05, **p < 0.01. ARHI, Aplasia Ras homolog member I.

false