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AB59769

Anti-E2F5 antibody [220D1a]

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(7 Publications)

Mouse Monoclonal E2F5 antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Transcription factor E2F5.

View Alternative Names

Transcription factor E2F5, E2F-5, E2F5

1 Images
Western blot - Anti-E2F5 antibody [220D1a] (AB59769)
  • WB

Unknown

Western blot - Anti-E2F5 antibody [220D1a] (AB59769)

All lanes:

Western blot - Anti-E2F5 antibody [220D1a] (ab59769)

All lanes:

immunizing peptide

Predicted band size: 38 kDa

Observed band size: 38 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

220D1a

Isotype

IgG1

Carrier free

No

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human Transcription factor E2F5. The exact immunogen used to generate this antibody is proprietary information.

Q15329

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Filtered through a 0.22 µm membrane.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

E2F5 also known simply as E2F-5 plays a mechanical role as a transcription factor involved in cell cycle regulation. It belongs to the E2F family known for regulating genes involved in cell division. E2F5 has a mass of approximately 36 kDa. It expresses in various tissues with a higher presence in the brain and placenta. This factor is widely recognized for its role in controlling the expression of genes essential for progression through the cell cycle.
Biological function summary

E2F5 functions as part of the mammalian E2F family which includes other transcription factors. E2F5 partners with proteins to form a complex often associating with DP proteins to bind DNA and regulate target gene expression. E2F5 is important for the control of cell proliferation differentiation and development processes by managing the transition from G1 to S phase followed by DNA replication. It coordinates with other E2F factors to ensure precise regulation of these cellular events.

Pathways

E2F5 plays a vital role in the E2F transcriptional network and the RB (retinoblastoma) pathway. This network critically impacts cell cycle progression where E2F5 interacts with members of the retinoblastoma protein family such as p130 to mediate transcriptional repression in the G0 phase. Additionally it is involved in pathways that link external signals to the control of cell cycle progression and development working with related proteins like E2F1 and E2F3 to maintain cellular function and integrity.

E2F5 shows connections to cancer and neurological disorders. In cancer due to its role in cell cycle regulation dysregulation of E2F5 can contribute to uncontrolled cell proliferation often linked with tumors. It frequently associates with proteins like p130 which when deregulated may result in abnormal cell growth. In neurological disorders aberrant expression of E2F5 in the brain suggests a potential link to neurodevelopmental abnormalities although its exact role requires further elucidation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Specifically required for multiciliate cell differentiation : together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
See full target information Transcription factor E2F5
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

BioMed research international 2022:6918396 PubMed35909488

2022

hsa_circ_0084811 Regulates Cell Proliferation and Apoptosis in Retinoblastoma through miR-18a-5p/miR-18b-5p/E2F5 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Guangwei Jiang,Mingxuan Qu,Lili Kong,Xiaoming Song,Shanhao Jiang

Cancer cell international 21:410 PubMed34348712

2021

CircFAM13B promotes the proliferation of hepatocellular carcinoma by sponging miR-212, upregulating E2F5 expression and activating the P53 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Xie,Xiaofeng Hang,Wensheng Xu,Jing Gu,Yuanjing Zhang,Jianrong Wang,Xiucui Zhang,Xinghao Cao,Junjie Zhan,Junxue Wang,Jianhe Gan

Journal of cellular physiology 230:3105-14 PubMed26012475

2015

The Effects of miR-20a on p21: Two Mechanisms Blocking Growth Arrest in TGF-β-Responsive Colon Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Viktorija Sokolova,Antonio Fiorino,Eugenio Zoni,Elisabetta Crippa,James F Reid,Manuela Gariboldi,Marco A Pierotti

PloS one 8:e85650 PubMed24392024

2013

A novel genetic system based on zinc finger nucleases for the identification of interactions between proteins in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Wang,Kun Xu,Juan Lin,Simin Shao,Tingting Zhang,Huarong Xu,Zehui Wei,Zhiying Zhang

PloS one 7:e35423 PubMed22523595

2012

A systems biology approach to characterize the regulatory networks leading to trabectedin resistance in an in vitro model of myxoid liposarcoma.

Applications

WB

Species

Unspecified reactive species

Sarah Uboldi,Enrica Calura,Luca Beltrame,Ilaria Fuso Nerini,Sergio Marchini,Duccio Cavalieri,Eugenio Erba,Giovanna Chiorino,Paola Ostano,Daniela D'Angelo,Maurizio D'Incalci,Chiara Romualdi

World journal of gastroenterology 17:470-7 PubMed21274376

2011

A potential oncogenic role of the commonly observed E2F5 overexpression in hepatocellular carcinoma.

Applications

IHC-P, WB

Species

Human, Human

Yuzhu Jiang,Seon-Hee Yim,Hai-Dong Xu,Seung-Hyun Jung,So Young Yang,Hae-Jin Hu,Chan-Kwon Jung,Yeun-Jun Chung

The Journal of clinical investigation 120:3530-44 PubMed20890043

2010

Human parvovirus B19 causes cell cycle arrest of human erythroid progenitors via deregulation of the E2F family of transcription factors.

Applications

WB

Species

Human

Zhihong Wan,Ning Zhi,Susan Wong,Keyvan Keyvanfar,Delong Liu,Nalini Raghavachari,Peter J Munson,Su Su,Daniela Malide,Sachiko Kajigaya,Neal S Young
View all publications
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