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AB227587

Anti-E2F5 antibody

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(1 Publication)

Rabbit Polyclonal E2F5 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Transcription factor E2F5.

View Alternative Names

Transcription factor E2F5, E2F-5, E2F5

3 Images
Western blot - Anti-E2F5 antibody (AB227587)
  • WB

Supplier Data

Western blot - Anti-E2F5 antibody (AB227587)

All lanes:

Western blot - Anti-E2F5 antibody (ab227587) at 1/500 dilution

Lane 1:

A549 Whole cell extract, at 30 µg

Lane 2:

H1299 Whole cell extract at 30 µg

Lane 3:

HCT116 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 38 kDa

false

Western blot - Anti-E2F5 antibody (AB227587)
  • WB

Supplier Data

Western blot - Anti-E2F5 antibody (AB227587)

All lanes:

Western blot - Anti-E2F5 antibody (ab227587) at 1/500 dilution

All lanes:

Mouse placenta tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 38 kDa

false

Western blot - Anti-E2F5 antibody (AB227587)
  • WB

Supplier Data

Western blot - Anti-E2F5 antibody (AB227587)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-E2F5 antibody (ab227587) at 1/10000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with HA-human E2F5, whole cell extract at 30 µg

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human Transcription factor E2F5. The exact immunogen used to generate this antibody is proprietary information.

Q15329

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

E2F5 also known simply as E2F-5 plays a mechanical role as a transcription factor involved in cell cycle regulation. It belongs to the E2F family known for regulating genes involved in cell division. E2F5 has a mass of approximately 36 kDa. It expresses in various tissues with a higher presence in the brain and placenta. This factor is widely recognized for its role in controlling the expression of genes essential for progression through the cell cycle.
Biological function summary

E2F5 functions as part of the mammalian E2F family which includes other transcription factors. E2F5 partners with proteins to form a complex often associating with DP proteins to bind DNA and regulate target gene expression. E2F5 is important for the control of cell proliferation differentiation and development processes by managing the transition from G1 to S phase followed by DNA replication. It coordinates with other E2F factors to ensure precise regulation of these cellular events.

Pathways

E2F5 plays a vital role in the E2F transcriptional network and the RB (retinoblastoma) pathway. This network critically impacts cell cycle progression where E2F5 interacts with members of the retinoblastoma protein family such as p130 to mediate transcriptional repression in the G0 phase. Additionally it is involved in pathways that link external signals to the control of cell cycle progression and development working with related proteins like E2F1 and E2F3 to maintain cellular function and integrity.

E2F5 shows connections to cancer and neurological disorders. In cancer due to its role in cell cycle regulation dysregulation of E2F5 can contribute to uncontrolled cell proliferation often linked with tumors. It frequently associates with proteins like p130 which when deregulated may result in abnormal cell growth. In neurological disorders aberrant expression of E2F5 in the brain suggests a potential link to neurodevelopmental abnormalities although its exact role requires further elucidation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Specifically required for multiciliate cell differentiation : together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
See full target information Transcription factor E2F5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell transplantation 31:9636897221081483 PubMed35236160

2022

The Roles of Different Multigene Combinations of , , , , and in the Reprogramming of Canine ADSCs Into IPCs.

Applications

Unspecified application

Species

Unspecified reactive species

Dengke Gao,Pengxiu Dai,Zhixin Fan,Jinglu Wang,Yihua Zhang
View all publications
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Product promise

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