AB53061

Anti-E2F6 antibody

Name: Anti-E2F6 antibody (ab53061) | Abcam
Brand: Abcam Limited
SKU: AB53061
Price: 535 USD
Availability: InStock
Rating: 5 (4 reviews)

(4 Reviews)

(15 Publications)

Rabbit Polyclonal E2F6 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human E2F6 aa 100-200.

View Alternative Names

Transcription factor E2F6, E2F-6, E2F6

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F6 antibody (AB53061)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F6 antibody (AB53061)

Immunohistochemistry analysis of paraffin-embedded human brain tissue labeling E2F6 with ab53061. The picture on the right is blocked with the synthesized peptide.

AbReview26125****

Western blot - Anti-E2F6 antibody (AB53061)

Blocking Step : 4% Milk for 1 hour at 22°C

All lanes:

Western blot - Anti-E2F6 antibody (ab53061) at 1/500 dilution

Lane 1:

HeLa cells

Lane 2:

Cell lysate (nuclear) of mouse embryonic stem cells at 100000 Cells

Secondary

All lanes:

HRP-conjugated Donkey anti rabbit IgG polyclonal at 1/20000 dilution

Predicted band size: 32 kDa

Observed band size: 35 kDa,65 kDa

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Exposure time: 10min

This image is courtesy of an Abreview submitted by Dr. Ligia Tavares

Supplier Data

Western blot - Anti-E2F6 antibody (AB53061)

All lanes:

Western blot - Anti-E2F6 antibody (ab53061) at 1/500 dilution

All lanes:

NIH/3T3 cell lysate

Predicted band size: 32 kDa

false

Supplier Data

Western blot - Anti-E2F6 antibody (AB53061)

All lanes:

Western blot - Anti-E2F6 antibody (ab53061) at 1/500 dilution

All lanes:

HepG2 cell lysate

Predicted band size: 32 kDa

false

Unknown

Western blot - Anti-E2F6 antibody (AB53061)

All lanes:

Western blot - Anti-E2F6 antibody (ab53061) at 1/500 dilution

Lane 1:

Extracts of HepG2 cells

Lane 2:

Extracts of HepG2 cells treated with peptide

Predicted band size: 32 kDa

Observed band size: 31 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human E2F6 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

O75461

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

E2F6 a member of the E2F family of transcription factors acts as a transcriptional repressor. It regulates genes essential for cell cycle progression particularly those involved in DNA replication. E2F6 lacks the transactivation domain present in other E2F family members allowing it uniquely to repress transcription without activating it. E2F6 has a molecular mass of approximately 30 kDa. The protein is mostly expressed in the nucleus of proliferating cells but it is also found in certain other tissues including the brain and testes.

Biological function summary

E2F6 plays an important role in regulating the cell cycle. It belongs to a multiprotein complex called the repressive E2F6 complex or PRC1 (Polycomb Repressive Complex 1). This complex is responsible for chromatin remodeling and transcriptional repression of target genes important in S phase entry and mitosis. By repressing specific E2F-responsive genes E2F6 inhibits excessive cell proliferation therefore maintaining proper cell cycle control.

Pathways

E2F6 contributes to important pathways like the cell cycle and DNA damage response pathways. In these pathways it is involved in controlling the transition phases of the cell cycle particularly regulating entry into the S phase. E2F6 interacts with proteins such as Rb (retinoblastoma protein) and other E2F members like E2F1 and E2F4 to control cell growth and ensure genome integrity.

Dysregulation of E2F6 has links to cancer and developmental disorders. In cancer overexpression or mutations affecting E2F6 can lead to uncontrolled cell proliferation due to improper repression of cell cycle genes. Additionally defects in the E2F6 gene have associations with developmental syndromes through interactions with other proteins like EZH2 a component of the Polycomb group proteins. These associations highlight the importance of E2F6 in both normal cell function and disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibitor of E2F-dependent transcription (PubMed : 9501179, PubMed : 9689056, PubMed : 9704927). Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' (PubMed : 9501179). Has a preference for the 5'-TTTCCCGC-3' E2F recognition site (PubMed : 9501179). E2F6 lacks the transcriptional activation and pocket protein binding domains (PubMed : 9501179, PubMed : 9704927). Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression (PubMed : 9501179, PubMed : 9689056). Represses expression of some meiosis-specific genes, including SLC25A31/ANT4 (By similarity). May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase (PubMed : 9501179).

See full target information E2F6

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 12: PubMed39767802

2025

Promoter Polymorphism (Allelic Variation) Affects Tacrolimus Treatment Efficacy by Modulating E2F6 Binding Affinity.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyi Zheng,Shengying Qin,Mingkang Zhong,Qinxia Xu,Cong Huai,Xiaoyan Qiu

NPJ precision oncology 8:243 PubMed39455812

2024

ALYREF recruits ELAVL1 to promote colorectal tumorigenesis via facilitating RNA m5C recognition and nuclear export.

Applications

Unspecified application

Species

Unspecified reactive species

Longhua Zhong,Jingxun Wu,Bingqian Zhou,Jiapeng Kang,Xicheng Wang,Feng Ye,Xiaoting Lin

Nature communications 14:4521 PubMed37607907

2023

TUG1-mediated R-loop resolution at microsatellite loci as a prerequisite for cancer cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Miho M Suzuki,Kenta Iijima,Koichi Ogami,Keiko Shinjo,Yoshiteru Murofushi,Jingqi Xie,Xuebing Wang,Yotaro Kitano,Akira Mamiya,Yuji Kibe,Tatsunori Nishimura,Fumiharu Ohka,Ryuta Saito,Shinya Sato,Junya Kobayashi,Ryoji Yao,Kanjiro Miyata,Kazunori Kataoka,Hiroshi I Suzuki,Yutaka Kondo

STAR protocols 3:101918 PubMed36595937

2022

An optimized chromatin immunoprecipitation protocol using Staph-seq for analyzing genome-wide protein-DNA interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Tao,Egidy Rhonda,Xi He,John M Perry,Linheng Li

Frontiers in oncology 11:648152 PubMed34381702

2021

The COX10-AS1/miR-641/E2F6 Feedback Loop Is Involved in the Progression of Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Liu,Xiaojian Li,Heming Wu,Yong Tang,Xiang Li,Yan Shi

eLife 10: PubMed34236315

2021

Loss of MGA repression mediated by an atypical polycomb complex promotes tumor progression and invasiveness.

Applications

Unspecified application

Species

Unspecified reactive species

Haritha Mathsyaraja,Jonathen Catchpole,Brian Freie,Emily Eastwood,Ekaterina Babaeva,Michael Geuenich,Pei Feng Cheng,Jessica Ayers,Ming Yu,Nan Wu,Sitapriya Moorthi,Kumud R Poudel,Amanda Koehne,William Grady,A McGarry Houghton,Alice H Berger,Yuzuru Shiio,David MacPherson,Robert N Eisenman

Oncology reports 46: PubMed34165171

2021

Long noncoding RNA SLC9A3‑AS1 increases E2F6 expression by sponging microRNA‑486‑5p and thus facilitates the oncogenesis of nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiansheng Li,Dongzhi Li,Xianhua Zhang,Cuijuan Li,Fengjuan Zhu

Nature communications 12:3582 PubMed34117224

2021

E2F6 initiates stable epigenetic silencing of germline genes during embryonic development.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Dahlet,Matthias Truss,Ute Frede,Hala Al Adhami,Anaïs F Bardet,Michael Dumas,Judith Vallet,Johana Chicher,Philippe Hammann,Sarah Kottnik,Peter Hansen,Uschi Luz,Gonzalo Alvarez,Ghislain Auclair,Jochen Hecht,Peter N Robinson,Christian Hagemeier,Michael Weber

Cell death and differentiation 28:2160-2178 PubMed33664480

2021

MACF1 promotes osteoblast differentiation by sequestering repressors in cytoplasm.

Applications

Unspecified application

Species

Unspecified reactive species

Lifang Hu,Chong Yin,Dong Chen,Zixiang Wu,Shujing Liang,Yu Zhang,Zizhan Huang,Shuyu Liu,Xia Xu,Zhihao Chen,Yi Zhang,Airong Qian

eLife 9: PubMed32985974

2020

Characterization of the mechanism by which the RB/E2F pathway controls expression of the cancer genomic DNA deaminase APOBEC3B.

Applications

Unspecified application

Species

Unspecified reactive species

Pieter A Roelofs,Chai Yeen Goh,Boon Haow Chua,Matthew C Jarvis,Teneale A Stewart,Jennifer L McCann,Rebecca M McDougle,Michael A Carpenter,John Wm Martens,Paul N Span,Dennis Kappei,Reuben S Harris

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-E2F6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F6 antibody (AB53061)

Western blot - Anti-E2F6 antibody (AB53061)

Western blot - Anti-E2F6 antibody (AB53061)

Western blot - Anti-E2F6 antibody (AB53061)

Western blot - Anti-E2F6 antibody (AB53061)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

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