Rabbit Polyclonal E3 SUMO-protein ligase PIAS4/PIASy antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PIAS4 aa 400-500.
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Publications
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- Frontiers in pharmacology 13:8720572022Dimethyl Fumarate Ameliorates Doxorubicin-Induced Cardiotoxicity By Activating the Nrf2 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoliang Hu,Cheng Li,Qian Wang,Zhixing Wei,Taizhong Chen,Yuepeng Wang,Yigang LiPubMed 35559248
- Antioxidants & redox signaling 32:636-6552020Regulation of the 20S Proteasome by a Novel Family of Inhibitory Proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaya A Olshina et. al.PubMed 31903784
- Journal of molecular cell biology 11:356-3702018Muscle RING-finger protein-1 (MuRF1) functions and cellular localization are regulated by SUMO1 post-translational modification.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGabriel Heras,Arvind Venkat Namuduri,Leonardo Traini,Ganna Shevchenko,Alexander Falk,Sara Bergström Lind,Mi Jia,Geng Tian,Stefano GastaldelloPubMed 29868881
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human PIAS4 aa 400-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N2W9
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation.
Alternative names
E3 SUMO-protein ligase PIAS4, PIASy, Protein inhibitor of activated STAT protein 4, Protein inhibitor of activated STAT protein gamma, RING-type E3 ubiquitin transferase PIAS4, PIAS-gamma, PIAS4, PIASG
Recommended products
Rabbit Polyclonal E3 SUMO-protein ligase PIAS4/PIASy antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PIAS4 aa 400-500.
Alternative names
E3 SUMO-protein ligase PIAS4, PIASy, Protein inhibitor of activated STAT protein 4, Protein inhibitor of activated STAT protein gamma, RING-type E3 ubiquitin transferase PIAS4, PIAS-gamma, PIAS4, PIASG
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human PIAS4 aa 400-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N2W9
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The E3 SUMO-protein ligase PIAS4 also known as PIASy functions as an E3-type small ubiquitin-like modifier (SUMO) ligase. This protein plays a role in SUMOylation a post-translational modification process where it catalyzes the transfer of SUMO from E2 conjugating enzyme to target substrates. PIAS4 has a mass of approximately 57 kDa and is expressed in various tissues including the brain heart and skeletal muscle. It shows localization in the nucleus reflecting its role in regulating nuclear processes.
- Biological function summary
This protein modulates transcription by interacting with and modifying transcription factors through SUMOylation affecting their stability and activity. PIAS4 participates in multiprotein complexes which can influence gene expression and cellular responses to stress. Its role in DNA repair processes is significant as it SUMOylates proteins involved in homologous recombination and DNA damage response pathways aiding in maintaining genomic integrity.
- Pathways
PIAS4 plays a role in the regulation of DNA damage repair and the STAT signaling pathway. It cooperates with proteins like BRCA1 and RAD51 in DNA repair ensuring the correct repair of double-strand breaks. In the STAT signaling pathway PIAS4 influences STAT transcription factors impacting various cellular activities including growth and apoptosis. This modulation links PIAS4 to cellular responses relevant to growth and stress.
- Associated diseases and disorders
PIAS4 has connections to cancer and neurodegenerative diseases. Mutations or altered expression of PIAS4 can disrupt DNA repair processes leading to an increased susceptibility to cancer due to accumulated genomic instability. PIAS4 also interacts with proteins like p53 in pathways associated with cancer development. Additionally its involvement in stress response mechanisms could link it to neurodegenerative diseases possibly through affecting neuronal cell survival and function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (ab137500)
10% SDS-PAGE
All lanes: Western blot - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (ab137500) at 1/5000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: PIAS4-transfected HEK-293T whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (ab137500)
Immunohistochemical analysis of paraffin-embedded DLD1 xenograft tissue staining E3 SUMO-protein ligase PIAS4 with ab137500 at a 1/100 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
Immunocytochemistry/ Immunofluorescence - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (ab137500)
Confocal immunofluorescence analysis analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell labelling E3 SUMO-protein ligase PIAS4 with ab137500 (green) at a 1/500 dilution. Alpha-tubulin filaments were labeled with an Alpha-tubulin antibody (red) at a 1/2000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (ab137500)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue staining E3 SUMO-protein ligase PIAS4 with ab137500 at a 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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