AB137500

Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody

Name: Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (ab137500) | Abcam
Brand: Abcam Limited
SKU: AB137500
Price: 545 USD
Availability: InStock

(1 Review)

(4 Publications)

Rabbit Polyclonal E3 SUMO-protein ligase PIAS4/PIASy antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PIAS4 aa 400-500.

View Alternative Names

PIASG, PIAS4, E3 SUMO-protein ligase PIAS4, PIASy, Protein inhibitor of activated STAT protein 4, Protein inhibitor of activated STAT protein gamma, PIAS-gamma

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (AB137500)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (AB137500)

Confocal immunofluorescence analysis analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell labelling E3 SUMO-protein ligase PIAS4 with ab137500 (green) at a 1/500 dilution. Alpha-tubulin filaments were labeled with an Alpha-tubulin antibody (red) at a 1/2000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (AB137500)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (AB137500)

Immunohistochemical analysis of paraffin-embedded DLD1 xenograft tissue staining E3 SUMO-protein ligase PIAS4 with ab137500 at a 1/100 dilution.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (AB137500)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue staining E3 SUMO-protein ligase PIAS4 with ab137500 at a 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (AB137500)

10% SDS-PAGE

All lanes:

Western blot - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (ab137500) at 1/5000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

PIAS4-transfected HEK-293T whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human PIAS4 aa 400-500. The exact immunogen used to generate this antibody is proprietary information.

Q8N2W9

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The E3 SUMO-protein ligase PIAS4 also known as PIASy functions as an E3-type small ubiquitin-like modifier (SUMO) ligase. This protein plays a role in SUMOylation a post-translational modification process where it catalyzes the transfer of SUMO from E2 conjugating enzyme to target substrates. PIAS4 has a mass of approximately 57 kDa and is expressed in various tissues including the brain heart and skeletal muscle. It shows localization in the nucleus reflecting its role in regulating nuclear processes.

Biological function summary

This protein modulates transcription by interacting with and modifying transcription factors through SUMOylation affecting their stability and activity. PIAS4 participates in multiprotein complexes which can influence gene expression and cellular responses to stress. Its role in DNA repair processes is significant as it SUMOylates proteins involved in homologous recombination and DNA damage response pathways aiding in maintaining genomic integrity.

Pathways

PIAS4 plays a role in the regulation of DNA damage repair and the STAT signaling pathway. It cooperates with proteins like BRCA1 and RAD51 in DNA repair ensuring the correct repair of double-strand breaks. In the STAT signaling pathway PIAS4 influences STAT transcription factors impacting various cellular activities including growth and apoptosis. This modulation links PIAS4 to cellular responses relevant to growth and stress.

PIAS4 has connections to cancer and neurodegenerative diseases. Mutations or altered expression of PIAS4 can disrupt DNA repair processes leading to an increased susceptibility to cancer due to accumulated genomic instability. PIAS4 also interacts with proteins like p53 in pathways associated with cancer development. Additionally its involvement in stress response mechanisms could link it to neurodegenerative diseases possibly through affecting neuronal cell survival and function.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor (PubMed : 12511558, PubMed : 12631292, PubMed : 12727872, PubMed : 15831457, PubMed : 15976810, PubMed : 22508508, PubMed : 32832608). Mediates sumoylation of ALKBH5, AXIN1, CEBPA, KLF8, GATA2, PARK7, HERC2, MYB, TCF4 and RNF168 (PubMed : 12223491, PubMed : 12511558, PubMed : 12631292, PubMed : 12727872, PubMed : 12750312, PubMed : 15831457, PubMed : 15976810, PubMed : 16617055, PubMed : 22508508, PubMed : 34048572). Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway (PubMed : 11388671). Involved in gene silencing (PubMed : 11248056). In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations (PubMed : 12727872, PubMed : 15831457). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed : 21965678). Binds to AT-rich DNA sequences, known as matrix or scaffold attachment regions (MARs/SARs) (By similarity). Catalyzes conjugation of SUMO2 to KAT5 in response to DNA damage, facilitating repair of DNA double-strand breaks (DSBs) via homologous recombination (HR) (PubMed : 32832608). Mediates sumoylation of PARP1 in response to PARP1 trapping to chromatin (PubMed : 35013556). Mediates sumoylation of KLF8, repressiing KLF8 transcriptional activity and cell cycle progression into G(1) phase (PubMed : 16617055). Sumoylates ALKBH5 downstream of MAPK8/JNK1 and MAPK9/JNK2 in response to reactive oxygen species (ROS), inhibiting ALKBH5 RNA demethylase activity (PubMed : 34048572).

See full target information PIAS4

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:674 PubMed39277582

2024

Impaired SUMOylation of FoxA1 promotes nonalcoholic fatty liver disease through down-regulation of Sirt6.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmei Zou,Jinwen Liao,Min Xiao,Liang Liu,Dongling Dai,Mingguo Xu

Frontiers in pharmacology 13:872057 PubMed35559248

2022

Dimethyl Fumarate Ameliorates Doxorubicin-Induced Cardiotoxicity By Activating the Nrf2 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoliang Hu,Cheng Li,Qian Wang,Zhixing Wei,Taizhong Chen,Yuepeng Wang,Yigang Li

Antioxidants & redox signaling 32:636-655 PubMed31903784

2020

Regulation of the 20S Proteasome by a Novel Family of Inhibitory Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Maya A Olshina,Galina Arkind,Fanindra Kumar Deshmukh,Irit Fainer,Mark Taranavsky,Daniel Hayat,Shifra Ben-Dor,Gili Ben-Nissan,Michal Sharon

Journal of molecular cell biology 11:356-370 PubMed29868881

2018

Muscle RING-finger protein-1 (MuRF1) functions and cellular localization are regulated by SUMO1 post-translational modification.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriel Heras,Arvind Venkat Namuduri,Leonardo Traini,Ganna Shevchenko,Alexander Falk,Sara Bergström Lind,Mi Jia,Geng Tian,Stefano Gastaldello

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody

Immunocytochemistry/ Immunofluorescence - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (AB137500)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (AB137500)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (AB137500)

Western blot - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (AB137500)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise