Rabbit Polyclonal E3 SUMO-protein ligase PIAS4/PIASy antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human PIAS4.
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- Molecular biology of the cell 34:br62023p97/VCP drives turnover of SUMOylated centromeric CCAN proteins and CENP-A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSebastiaan J W van den Berg,Samuel East,Sreyoshi Mitra,Lars E T JansenPubMed 36989032
- Nucleic acids research 50:3922-39432022ZMYM2 restricts 53BP1 at DNA double-strand breaks to favor BRCA1 loading and homologous recombination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDoohyung Lee,Katja Apelt,Seong-Ok Lee,Hsin-Ru Chan,Martijn S Luijsterburg,Justin W C Leung,Kyle M MillerPubMed 35253893
- Science advances 6:eaba78222020TIP60 K430 SUMOylation attenuates its interaction with DNA-PKcs in S-phase cells: Facilitating homologous recombination and emerging target for cancer therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShan-Shan Gao et. al.PubMed 32832608
- Nucleus (Austin, Tex.) 9:87-942017SUMO modification system facilitates the exchange of histone variant H2A.Z-2 at DNA damage sites.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAtsuhiko Fukuto,Masae Ikura,Tsuyoshi Ikura,Jiying Sun,Yasunori Horikoshi,Hiroki Shima,Kazuhiko Igarashi,Masayuki Kusakabe,Masahiko Harata,Naoki Horikoshi,Hitoshi Kurumizaka,Yoshiaki Kiuchi,Satoshi TashiroPubMed 29095668
- Nature communications 6:89792015SUMOylation of AMPKα1 by PIAS4 specifically regulates mTORC1 signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Yan et. al.PubMed 26616021
- The Journal of cell biology 193:867-842011PKCζ mediates disturbed flow-induced endothelial apoptosis via p53 SUMOylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKyung-Sun Heo,Hakjoo Lee,Patrizia Nigro,Tamlyn Thomas,Nhat-Tu Le,Eugene Chang,Carolyn McClain,Cynthia A Reinhart-King,Michael R King,Bradford C Berk,Keigi Fujiwara,Chang-Hoon Woo,Jun-ichi AbePubMed 21624955
- The Journal of biological chemistry 286:14830-412011Caveolin-3 undergoes SUMOylation by the SUMO E3 ligase PIASy: sumoylation affects G-protein-coupled receptor desensitization.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human PIAS4. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N2W9
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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Target data
Function
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation.
Alternative names
E3 SUMO-protein ligase PIAS4, PIASy, Protein inhibitor of activated STAT protein 4, Protein inhibitor of activated STAT protein gamma, RING-type E3 ubiquitin transferase PIAS4, PIAS-gamma, PIAS4, PIASG
Recommended products
Rabbit Polyclonal E3 SUMO-protein ligase PIAS4/PIASy antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human PIAS4.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human PIAS4. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N2W9
- Purification technique
Affinity purification Immunogen
- Specificity
Detects endogenous levels of PAIAS gamma total protein.
- Concentration
- Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The E3 SUMO-protein ligase PIAS4 also known as PIASy functions as an E3-type small ubiquitin-like modifier (SUMO) ligase. This protein plays a role in SUMOylation a post-translational modification process where it catalyzes the transfer of SUMO from E2 conjugating enzyme to target substrates. PIAS4 has a mass of approximately 57 kDa and is expressed in various tissues including the brain heart and skeletal muscle. It shows localization in the nucleus reflecting its role in regulating nuclear processes.
- Biological function summary
This protein modulates transcription by interacting with and modifying transcription factors through SUMOylation affecting their stability and activity. PIAS4 participates in multiprotein complexes which can influence gene expression and cellular responses to stress. Its role in DNA repair processes is significant as it SUMOylates proteins involved in homologous recombination and DNA damage response pathways aiding in maintaining genomic integrity.
- Pathways
PIAS4 plays a role in the regulation of DNA damage repair and the STAT signaling pathway. It cooperates with proteins like BRCA1 and RAD51 in DNA repair ensuring the correct repair of double-strand breaks. In the STAT signaling pathway PIAS4 influences STAT transcription factors impacting various cellular activities including growth and apoptosis. This modulation links PIAS4 to cellular responses relevant to growth and stress.
- Associated diseases and disorders
PIAS4 has connections to cancer and neurodegenerative diseases. Mutations or altered expression of PIAS4 can disrupt DNA repair processes leading to an increased susceptibility to cancer due to accumulated genomic instability. PIAS4 also interacts with proteins like p53 in pathways associated with cancer development. Additionally its involvement in stress response mechanisms could link it to neurodegenerative diseases possibly through affecting neuronal cell survival and function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (ab58416)
All lanes: Western blot - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (ab58416) at 1/500 dilution
Lane 1: Jurkat cell extract without immunizing peptide
Lane 2: Jurkat cell extract with immunizing peptide
Predicted band size: 56 kDa
Observed band size: 57 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (ab58416)
IHC image of ab58416 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58416, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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