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AB238677

Anti-E3 ubiquitin-protein ligase RNF213 antibody

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(1 Publication)

Rabbit Polyclonal C17orf27 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RNF213 aa 200 to C-terminus.

View Alternative Names

ALO17, C17orf27, KIAA1554, KIAA1618, MYSTR, RNF213, E3 ubiquitin-protein ligase RNF213, ALK lymphoma oligomerization partner on chromosome 17, E3 ubiquitin-lipopolysaccharide ligase RNF213, Mysterin, RING finger protein 213

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E3 ubiquitin-protein ligase RNF213 antibody (AB238677)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E3 ubiquitin-protein ligase RNF213 antibody (AB238677)

Paraffin-embedded human colon cancer tissue stained for RNF213 using ab238677 at 1/800 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunocytochemistry/ Immunofluorescence - Anti-E3 ubiquitin-protein ligase RNF213 antibody (AB238677)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-E3 ubiquitin-protein ligase RNF213 antibody (AB238677)

A549 (human lung carcinoma cell line) cells stained for RNF213 (green) using ab238677 at 1/266 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear counterstain was DAPI (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E3 ubiquitin-protein ligase RNF213 antibody (AB238677)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E3 ubiquitin-protein ligase RNF213 antibody (AB238677)

Paraffin-embedded human gastric cancer tissue stained for RNF213 using ab238677 at 1/800 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RNF213 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q63HN8

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB320820

Anti-UBR4/p600 antibody [EPR28593-48] - BSA and Azide free

BOND RX™ Validated|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBR4/p600 antibody [EPR28593-48] - BSA and Azide free (AB320820)

  • 0
  • 0
Primary Antibodies

AB180170

Anti-RING1 antibody [EPR13047]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-RING1 antibody [EPR13047] (AB180170)

  • 0
  • 0
Primary Antibodies

AB91464

Anti-C13orf7 antibody

Immunoprecipitation - Anti-C13orf7 antibody (AB91464)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

E3 ubiquitin-protein ligase RNF213 also known as mysterin functions as an E3 ubiquitin ligase in the ubiquitin-proteasome system. This large protein has a molecular mass of around 591 kDa. RNF213 mediates the ubiquitination of substrate proteins which marks them for degradation. This enzyme expresses mainly in endothelial and smooth muscle cells with some presence in other tissues. RNF213's role in protein degradation is essential for cellular regulation and protein turnover.
Biological function summary

RNF213 plays a significant function in vascular homeostasis and cellular stress response. It does not typically form part of a larger protein complex but interacts directly with its substrates during ubiquitination. Its activity is important for managing cellular processes linked to cell survival under various stress conditions. RNF213 modifies the stability and activity of proteins involved in these processes which supports proper vascular function and stress management.

Pathways

RNF213 plays a role in the autophagy and hypoxia-response pathways. Through these pathways RNF213 regulates proteins like hypoxia-inducible factor 1-alpha (HIF-1α) and other substrates that are integral to cellular response to low oxygen levels. This regulation contributes to cellular adaptation to stress conditions and affects pathways related to protein turnover and stress signal transduction.

RNF213 mutations associate with Moyamoya disease a rare cerebrovascular disorder and may also relate to some forms of angiogenesis-related pathologies. In Moyamoya disease altered RNF213 affects vascular remodeling and leads to progressive stenosis of the cerebral arteries. Other proteins such as p53 which interact with RNF213 play roles in cellular response to stress indicating possible links to other types of vascular disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Atypical E3 ubiquitin ligase that can catalyze ubiquitination of both proteins and lipids, and which is involved in various processes, such as lipid metabolism, angiogenesis and cell-autonomous immunity (PubMed : 21799892, PubMed : 26126547, PubMed : 26278786, PubMed : 26766444, PubMed : 30705059, PubMed : 32139119, PubMed : 34012115). Acts as a key immune sensor by catalyzing ubiquitination of the lipid A moiety of bacterial lipopolysaccharide (LPS) via its RZ-type zinc-finger : restricts the proliferation of cytosolic bacteria, such as Salmonella, by generating the bacterial ubiquitin coat through the ubiquitination of LPS (PubMed : 34012115). Also acts indirectly by mediating the recruitment of the LUBAC complex, which conjugates linear polyubiquitin chains (PubMed : 34012115). Ubiquitination of LPS triggers cell-autonomous immunity, such as antibacterial autophagy, leading to degradation of the microbial invader (PubMed : 34012115). Involved in lipid metabolism by regulating fat storage and lipid droplet formation; act by inhibiting the lipolytic process (PubMed : 30705059). Also regulates lipotoxicity by inhibiting desaturation of fatty acids (PubMed : 30846318). Also acts as an E3 ubiquitin-protein ligase via its RING-type zinc finger : mediates 'Lys-63'-linked ubiquitination of target proteins (PubMed : 32139119, PubMed : 33842849). Involved in the non-canonical Wnt signaling pathway in vascular development : acts by mediating ubiquitination and degradation of FLNA and NFATC2 downstream of RSPO3, leading to inhibit the non-canonical Wnt signaling pathway and promoting vessel regression (PubMed : 26766444). Also has ATPase activity; ATPase activity is required for ubiquitination of LPS (PubMed : 34012115).
See full target information RNF213
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Toxins 14: PubMed35050979

2021

Molecular Characterization and Functional Analysis of the Nattectin-like Toxin from the Venomous Fish .

Applications

Unspecified application

Species

Unspecified reactive species

Monica Lopes-Ferreira,Ines Sosa-Rosales,Pedro Ismael Silva Junior,Katia Conceicao,Adolfo Luis Almeida Maleski,Leticia Balan-Lima,Geonildo Rodrigo Disner,Carla Lima
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Product promise

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