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AB70618

Anti-E4F1 antibody

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(1 Publication)

Rabbit Polyclonal E4F1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human E4F1 aa 700 to C-terminus.

View Alternative Names

E4F, E4F1, Transcription factor E4F1, E4F transcription factor 1, Putative E3 ubiquitin-protein ligase E4F1, RING-type E3 ubiquitin transferase E4F1, Transcription factor E4F, p120E4F, p50E4F

1 Images
Western blot - Anti-E4F1 antibody (AB70618)
  • WB

Unknown

Western blot - Anti-E4F1 antibody (AB70618)

All lanes:

Western blot - Anti-E4F1 antibody (ab70618) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

MCF7 whole cell lysate at 50 µg

Predicted band size: 84 kDa

Observed band size: 120 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human E4F1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q66K89

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab70618 was affinity purified using an epitope specific to E4F1 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

E4F1 also known as adenovirus E4 promoter-binding protein 1 is a transcriptional regulator with a molecular mass of approximately 120 kDa. This protein is expressed in both the nucleus and cytoplasm of cells. E4F1 plays an essential role in regulating gene expression by modulating transcription factors and interacting with DNA. It is known for its ability to bind to promoters influencing the transcriptional initiation of target genes.
Biological function summary

The role of E4F1 involves cell cycle control apoptosis and DNA repair. E4F1 interacts with a network of proteins forming complexes that are important for its function. These complexes enable E4F1 to influence cellular responses to stress and aid in DNA damage repair mechanisms. E4F1's impact on these biological processes underlines its significance in maintaining cell homeostasis and genomic stability.

Pathways

E4F1 is significantly involved in the p53 signaling pathway and cell cycle control mechanisms. E4F1's role in these pathways is closely connected with the tumor suppressor protein p53 where it modulates p53’s transcriptional activity under stress conditions. Additionally E4F1 interacts with Rb (retinoblastoma protein) in the regulation of cell cycle progression especially during the transition from the G1 to S phase accentuating its contribution to cell cycle checkpoints.

E4F1 has a notable connection with cancer and neurodegenerative conditions. In cancer E4F1’s interaction with p53 influences tumorigenesis as perturbations in this interaction can lead to uncontrolled cell proliferation. In neurodegenerative diseases E4F1’s involvement in DNA repair and apoptosis links it to pathway disruptions that are characteristic of such disorders. E4F1's relationships with proteins like p53 and Rb suggest its potential as both a biomarker and therapeutic target in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May function as a transcriptional repressor. May also function as a ubiquitin ligase mediating ubiquitination of chromatin-associated TP53. Functions in cell survival and proliferation through control of the cell cycle. Functions in the p53 and pRB tumor suppressor pathways and regulates the cyclin CCNA2 transcription.. Identified as a cellular target of the adenoviral oncoprotein E1A, it is required for both transcriptional activation and repression of viral genes.
See full target information E4F1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 9:313 PubMed37626049

2023

BRD7 suppresses tumor chemosensitivity to CHK1 inhibitors by inhibiting USP1-mediated deubiquitination of CHK1.

Applications

Unspecified application

Species

Unspecified reactive species

Lemin Li,Linchen Wang,Dian Liu,Yongchao Zhao
View all publications
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Product promise

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