AB235203

Anti-EAAT1 antibody

Name: Anti-EAAT1 antibody (ab235203) | Abcam
Brand: Abcam Limited
SKU: AB235203
Price: 525 USD
Availability: InStock

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(1 Publication)

Chicken Polyclonal EAAT1 antibody. Suitable for WB, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 1 publication.

View Alternative Names

EAAT1, GLAST, GLAST1, SLC1A3, Excitatory amino acid transporter 1, Sodium-dependent glutamate/aspartate transporter 1, Solute carrier family 1 member 3, GLAST-1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-EAAT1 antibody (AB235203)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EAAT1 antibody (AB235203)

4% PFA fixed, 0.15% Triton X-100 permeabilized post-natal rat pup (PND1) heterogeneous brain cells* labeling EAAT1 using ab235203 at 15 μg/ml, O/N at +4°C (green) followed by Goat Anti-Chicken IgG DyLight™ 488 conjugated preadsorbed at 5 μg/ml for 1 h at RT, in ICC/IF. Nuclear probe is visualized in blue (DAPI).

*UniprotID Expression Tissue Specificity and Subcellular Localization, as well as additional information found in Human Protein Atlas.

Expected localization : Cell membrane (mitochondria, nucleus)
Observed localization : punctate staining indicates mitochondrial detection**

**Staining as confirmed in Gensat for Slc1a3 (high affinity glial glutamate transporter) : https : //www.gensat.org/imagenavigator_html/ImageNavigatorV2_10581.html

Supplier Data

Western blot - Anti-EAAT1 antibody (AB235203)

*UniprotID Expression Tissue Specificity : Detected in brain and cerebellum (PubMed : 1279699, PubMed : 8387171). Both isoform EAAT1/GLAST-1 and EAAT1/GLAST-1A are expressed in bone and brain (PubMed : 11086157). In brain isoform EAAT1/GLAST-1 is highly enriched in the Purkinje cell layer in cerebellum (PubMed : 11086157).

Minimal cortex defined : minimal cortex brain samples have had a portion of the cortex removed, with 1/3 cortex, cerebellum and other brain components present in the final lysate.

All lanes:

Western blot - Anti-EAAT1 antibody (ab235203) at 1/500 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 20 µg

Lane 2:

Adult mouse whole liver lysate at 20 µg

Lane 3:

Rat pup PND2-6 - minimal cortex brain lysate* at 20 µg

Lane 4:

Rat pup PND2-6 - minimal cortex brain lysate* at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-chicken-igy-h-l-hrp-preadsorbed-ab7118'>ab7118</a>) at 1/40000 dilution

Predicted band size: 59 kDa

Observed band size: 54 kDa

false

Exposure time: 60s

Key facts

Host species

Chicken

Clonality

Polyclonal

Isotype

IgY

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "15 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "15 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

IgY fraction

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EAAT1 also known as Excitatory Amino Acid Transporter 1 or anti-GLAST plays a critical role in mediating the uptake of glutamate from the synaptic cleft into glial cells. This transporter helps maintain the delicate balance of excitatory neurotransmission in the central nervous system. EAAT1 has a molecular mass of approximately 57 kDa. You will find EAAT1 expressed predominantly in astrocytes within the brain and retina where it facilitates the regulation of extracellular concentrations of glutamate preventing excitotoxicity.

Biological function summary

EAAT1 is instrumental in maintaining low levels of extracellular glutamate acting as a protective mechanism against neurotoxicity. This transporter does not operate as part of a larger protein complex instead functioning independently to efficiently clear synaptic glutamate. By ensuring rapid uptake of glutamate EAAT1 helps maintain normal synaptic transmission and neuronal communication. The action of EAAT1 is important for preventing excessive activation of glutamate receptors which can lead to cellular damage.

Pathways

EAAT1 is heavily involved in the glutamatergic neurotransmission pathway which is essential for various brain functions including learning and memory. EAAT1 acts in coordination with other proteins like EAAT2 to manage glutamate levels within the synaptic cleft. Another pathway where EAAT1 plays a role is the glutamate-glutamine cycle where it works alongside proteins such as glutamine synthetase to recycle glutamate and sustain neurotransmitter balance. This interplay supports neuronal health and protects against glutamate excitotoxicity.

EAAT1 has been implicated in neurological conditions such as epilepsy and Alzheimer's disease. The dysfunction or altered expression of EAAT1 can lead to insufficient glutamate clearance contributing to the pathophysiology of these disorders. In epilepsy the decreased function of EAAT1 may result in abnormally high levels of synaptic glutamate enhancing the risk of seizures. Similarly in Alzheimer's altered EAAT1 activity can exacerbate neurodegenerative processes. EAAT1's role is tightly linked with proteins like NMDA receptors and EAAT2 which further influence disease progression by affecting glutamate dynamics.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed : 20477940, PubMed : 26690923, PubMed : 28032905, PubMed : 28424515, PubMed : 7521911, PubMed : 8123008). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (PubMed : 20477940). Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (PubMed : 20477940). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity).

See full target information SLC1A3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biology 14: PubMed41007382

2025

A Simple Three-Dimensional Compartmentalized Co-Culture Model for Basal Forebrain and Hippocampal Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoman Luo,Jing Li,Zhiyu Deng,Yali Xu,Xixi Li,Miao Ren,Xiangning Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EAAT1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-EAAT1 antibody (AB235203)

Western blot - Anti-EAAT1 antibody (AB235203)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise