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Rabbit Recombinant Monoclonal EAAT1 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Rat, Human samples. Cited in 10 publications.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFWB
Human
Tested
Not recommended
Tested
Mouse
Tested
Tested
Tested
Rat
Tested
Tested
Expected
Monkey
Predicted
Not recommended
Predicted

Tested
Tested

Species

Mouse

Dilution info

1/50 - 1/1000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/50 - 1/1000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/50 - 1/1000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

-

Species

Rat

Dilution info

1/50

Notes

-

Not recommended
Not recommended

Species

Human, Monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/10000

Notes

-

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Monkey

Dilution info

-

Notes

-

Target data

Function

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7521911, PubMed:8123008, PubMed:20477940, PubMed:26690923, PubMed:28032905, PubMed:28424515). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (PubMed:20477940). Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (PubMed:20477940). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity).

Alternative names

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Rabbit Recombinant Monoclonal EAAT1 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Rat, Human samples. Cited in 10 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR12686

Purification technique

Affinity purification Protein A

Specificity

Unsuitable for human ICC/IF.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

EAAT1 is instrumental in maintaining low levels of extracellular glutamate acting as a protective mechanism against neurotoxicity. This transporter does not operate as part of a larger protein complex instead functioning independently to efficiently clear synaptic glutamate. By ensuring rapid uptake of glutamate EAAT1 helps maintain normal synaptic transmission and neuronal communication. The action of EAAT1 is important for preventing excessive activation of glutamate receptors which can lead to cellular damage.

Activity summary

EAAT1 also known as Excitatory Amino Acid Transporter 1 or anti-GLAST plays a critical role in mediating the uptake of glutamate from the synaptic cleft into glial cells. This transporter helps maintain the delicate balance of excitatory neurotransmission in the central nervous system. EAAT1 has a molecular mass of approximately 57 kDa. You will find EAAT1 expressed predominantly in astrocytes within the brain and retina where it facilitates the regulation of extracellular concentrations of glutamate preventing excitotoxicity.

Pathways

EAAT1 is heavily involved in the glutamatergic neurotransmission pathway which is essential for various brain functions including learning and memory. EAAT1 acts in coordination with other proteins like EAAT2 to manage glutamate levels within the synaptic cleft. Another pathway where EAAT1 plays a role is the glutamate-glutamine cycle where it works alongside proteins such as glutamine synthetase to recycle glutamate and sustain neurotransmitter balance. This interplay supports neuronal health and protects against glutamate excitotoxicity.

Associated diseases and disorders

EAAT1 has been implicated in neurological conditions such as epilepsy and Alzheimer's disease. The dysfunction or altered expression of EAAT1 can lead to insufficient glutamate clearance contributing to the pathophysiology of these disorders. In epilepsy the decreased function of EAAT1 may result in abnormally high levels of synaptic glutamate enhancing the risk of seizures. Similarly in Alzheimer's altered EAAT1 activity can exacerbate neurodegenerative processes. EAAT1's role is tightly linked with proteins like NMDA receptors and EAAT2 which further influence disease progression by affecting glutamate dynamics.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

  • Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036), expandable thumbnail

    Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    We recommend not to boil the samples after lysis to get desired WB results.

    All lanes: Western blot - Anti-EAAT1 antibody [EPR12686] (AB181036) at 1/5000 dilution

    Lane 1: Mouse brain lysate boiled at 15 µg

    Lane 2: Mouse brain lysate unboiled at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 59 kDa

    Exposure time: 10s

  • Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036), expandable thumbnail

    Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)

    All lanes: Western blot - Anti-EAAT1 antibody [EPR12686] (AB181036) at 1/5000 dilution

    Lane 1: Mouse brain at 20 µg

    Lane 2: Mouse hippocampus at 20 µg

    Lane 3: Rat hippocampus at 20 µg

    Lane 4: Rat brain at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 59 kDa

    Observed band size: 59 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

    ab181036 staining EAAT1 in mouse cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-EAAT1 antibody [EPR12686] (ab181036), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-EAAT1 antibody [EPR12686] (ab181036)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural / glia cells labelling EAAT1 with ab181036 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in mouse primary glia cell. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-EAAT1 antibody [EPR12686] (ab181036), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-EAAT1 antibody [EPR12686] (ab181036)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural / glia cells labelling EAAT1 with ab181036 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in mouse primary neural / glia cell. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/ml dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

    ab181036 staining EAAT1 in rat cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

    ab181036 staining EAAT1 in human glioma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

    ab181036 staining EAAT1 in human cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036), expandable thumbnail

    Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)

    Blocking and diluting buffer: 5% NFDM/TBST

    The band above (100KDa) is dimer

    All lanes: Western blot - Anti-EAAT1 antibody [EPR12686] (AB181036) at 1/5000 dilution

    All lanes: Human cerebellum at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 143 kDa, 26 kDa, 59 kDa

    Observed band size: 150 kDa, 59 kDa

  • Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036), expandable thumbnail

    Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)

    All lanes: Western blot - Anti-EAAT1 antibody [EPR12686] (AB181036) at 1/1000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Rat brain lysate at 10 µg

    Lane 3: Human cerebellum lysate at 10 µg

    Predicted band size: 59 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

    Immunohistochemical staining of EAAT1 in paraffin-embedded human brain tissue using ab181036 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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