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AB235202

Anti-EAAT2 antibody

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(1 Publication)

Goat Polyclonal EAAT2 antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication.

View Alternative Names

EAAT2, GLT1, SLC1A2, Excitatory amino acid transporter 2, Glutamate/aspartate transporter II, Sodium-dependent glutamate/aspartate transporter 2, Solute carrier family 1 member 2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-EAAT2 antibody (AB235202)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EAAT2 antibody (AB235202)

4% paraformaldehyde-fixed, 0.3% Triton X-100 permeabilized rat pup brain heterogenous cells stained for EAAT2 (green) using ab235202 at 15 μg/ml (overnight at 4°C) in ICC/IF. Donkey Anti-Goat IgG DyLight™ 488 Preadsorbed was used as the secondary antibody 5 μg/ml (1 hour at RT). Nuclear counterstain was DAPI.

Western blot - Anti-EAAT2 antibody (AB235202)
  • WB

Supplier Data

Western blot - Anti-EAAT2 antibody (AB235202)

Predicted MW : 60, 62 kDa (glycosylations and lipidations).

Dimerization expected.

All lanes:

Western blot - Anti-EAAT2 antibody (ab235202) at 5 µg/mL

Lane 1:

Rat brain lysate at 35 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 35 µg

Secondary

All lanes:

Goat IgG [H&L] HRP at 1/40000 dilution

Predicted band size: 62 kDa

false

Exposure time: 60s

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Recommended for rat samples only.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5 µg/mL", "WB-species-notes": "<p>Predicted MW: 60, 62 kDa (glycosylations and lipidations).</p><p>Dimerization expected.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5 µg/mL", "WB-species-notes": "<p>Predicted MW: 60, 62 kDa (glycosylations and lipidations).</p><p>Dimerization expected.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "15 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EAAT2 also known as excitatory amino acid transporter 2 or GLT-1 is a protein responsible for reuptake of glutamate from the synaptic cleft into glial cells preventing excitotoxicity. The protein has a mass of approximately 73 kDa and prominently expresses in the central nervous system particularly in astrocytes. By regulating extracellular glutamate levels EAAT2 helps maintain neurotransmitter balance critical for healthy neuron function.
Biological function summary

EAAT2 plays an important role in maintaining synaptic transmission and preventing overexcitation that can lead to neuronal damage. It is part of a transporter complex responsible for the movement of glutamate across the cell membrane working together with ions like sodium and potassium. Due to its significant role in the regulation of neurotransmitter levels in the brain EAAT2 is considered an important player in neural communication processes.

Pathways

EAAT2 is integral to the glutamatergic signaling pathway where its activity supports glutamate recycling and homeostasis. This pathway is important for normal cognitive functions such as learning and memory. EAAT2 works closely with other proteins such as EAAT1 in efforts to regulate glutamate concentrations in the extracellular space impacting synaptic strength and plasticity in the nervous system.

Malfunctions of EAAT2 are associated with conditions like amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. In ALS EAAT2 dysfunction leads to accumulation of neurotoxic levels of glutamate causing motor neuron death. Similarly in Alzheimer’s disease impaired regulation of EAAT2 contributes to neurodegeneration through a related increase in neuronal excitotoxicity. Both conditions emphasize the critical importance of EAAT2 in neuroprotection and maintenance of normal brain function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed : 14506254, PubMed : 15265858, PubMed : 26690923, PubMed : 7521911). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (PubMed : 14506254). Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (PubMed : 14506254). Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate (By similarity).
See full target information SLC1A2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biology 14: PubMed41007382

2025

A Simple Three-Dimensional Compartmentalized Co-Culture Model for Basal Forebrain and Hippocampal Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoman Luo,Jing Li,Zhiyu Deng,Yali Xu,Xixi Li,Miao Ren,Xiangning Li
View all publications
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Product promise

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