Goat Polyclonal EAAT2 antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples.
Preservative: 0.01% Sodium azide
Constituents: PBS
WB | ICC/IF | |
---|---|---|
Human | Tested | Expected |
Rat | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 5 µg/mL | Notes Predicted MW: 60, 62 kDa (glycosylations and lipidations). Dimerization expected. |
Species Human | Dilution info 5 µg/mL | Notes Predicted MW: 60, 62 kDa (glycosylations and lipidations). Dimerization expected. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 15 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
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Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:14506254, PubMed:15265858, PubMed:26690923, PubMed:7521911). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (PubMed:14506254). Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (PubMed:14506254). Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate (By similarity).
EAAT2, GLT1, SLC1A2, Excitatory amino acid transporter 2, Glutamate/aspartate transporter II, Sodium-dependent glutamate/aspartate transporter 2, Solute carrier family 1 member 2
Goat Polyclonal EAAT2 antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples.
Preservative: 0.01% Sodium azide
Constituents: PBS
Recommended for rat samples only.
EAAT2 also known as excitatory amino acid transporter 2 or GLT-1 is a protein responsible for reuptake of glutamate from the synaptic cleft into glial cells preventing excitotoxicity. The protein has a mass of approximately 73 kDa and prominently expresses in the central nervous system particularly in astrocytes. By regulating extracellular glutamate levels EAAT2 helps maintain neurotransmitter balance critical for healthy neuron function.
EAAT2 plays an important role in maintaining synaptic transmission and preventing overexcitation that can lead to neuronal damage. It is part of a transporter complex responsible for the movement of glutamate across the cell membrane working together with ions like sodium and potassium. Due to its significant role in the regulation of neurotransmitter levels in the brain EAAT2 is considered an important player in neural communication processes.
EAAT2 is integral to the glutamatergic signaling pathway where its activity supports glutamate recycling and homeostasis. This pathway is important for normal cognitive functions such as learning and memory. EAAT2 works closely with other proteins such as EAAT1 in efforts to regulate glutamate concentrations in the extracellular space impacting synaptic strength and plasticity in the nervous system.
Malfunctions of EAAT2 are associated with conditions like amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. In ALS EAAT2 dysfunction leads to accumulation of neurotoxic levels of glutamate causing motor neuron death. Similarly in Alzheimer’s disease impaired regulation of EAAT2 contributes to neurodegeneration through a related increase in neuronal excitotoxicity. Both conditions emphasize the critical importance of EAAT2 in neuroprotection and maintenance of normal brain function.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Predicted MW: 60, 62 kDa (glycosylations and lipidations).
Dimerization expected.
All lanes: Western blot - Anti-EAAT2 antibody (ab235202) at 5 µg/mL
Lane 1: Rat brain lysate at 35 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 35 µg
All lanes: Goat IgG [H&L] HRP at 1/40000 dilution
Predicted band size: 62 kDa
Exposure time: 60s
4% paraformaldehyde-fixed, 0.3% Triton X-100 permeabilized rat pup brain heterogenous cells stained for EAAT2 (green) using ab235202 at 15 μg/ml (overnight at 4°C) in ICC/IF. Donkey Anti-Goat IgG DyLight™ 488 Preadsorbed was used as the secondary antibody 5 μg/ml (1 hour at RT). Nuclear counterstain was DAPI.
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