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Anti-EAAT2 antibody (ab41621) is a rabbit polyclonal antibody that is used to detect EAAT2 in Western Blot. Suitable for Mouse, Rat samples.



- Over 80 publications

- Trusted since 2007


Images

Western blot - Anti-EAAT2 antibody (AB41621), expandable thumbnail
  • Immunocytochemistry - Anti-EAAT2 antibody (AB41621), expandable thumbnail
  • Immunocytochemistry - Anti-EAAT2 antibody (AB41621), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form
Liquid
Clonality
Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCWB
Mouse
Expected
Tested
Rat
Expected
Tested

Expected
Expected

Species
Rat
Dilution info
10 µg/mL
Notes

-

Species
Mouse
Dilution info
10 µg/mL
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1 µg/mL
Notes

-

Species
Rat
Dilution info
1 µg/mL
Notes

-

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Target data

Function

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7557442, PubMed:7698742, PubMed:9373176). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (By similarity). Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate (PubMed:9180080).

Alternative names

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Anti-EAAT2 antibody (ab41621) is a rabbit polyclonal antibody that is used to detect EAAT2 in Western Blot. Suitable for Mouse, Rat samples.



- Over 80 publications

- Trusted since 2007

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique
Affinity purification Immunogen
Specificity

From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-EAAT2 antibody (ab41621) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Mouse, Rat samples.

What is the molecular weight of EAAT2?


Anti-EAAT2 (ab41621) specifically detects a band for EAAT2 (UniProt: P31596) at a molecular weight of 62kDa.

Trusted by the scientific community


Anti-EAAT2 (ab41621) was first used in a scientific publication in 2007 and has been cited over 80 times in peer-reviewed journals.

Reviewed by scientists


Anti-EAAT2 (ab41621) has over 5 independent reviews from customers.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

EAAT2 also known as excitatory amino acid transporter 2 or GLT-1 is a protein responsible for reuptake of glutamate from the synaptic cleft into glial cells preventing excitotoxicity. The protein has a mass of approximately 73 kDa and prominently expresses in the central nervous system particularly in astrocytes. By regulating extracellular glutamate levels EAAT2 helps maintain neurotransmitter balance critical for healthy neuron function.

Biological function summary

EAAT2 plays an important role in maintaining synaptic transmission and preventing overexcitation that can lead to neuronal damage. It is part of a transporter complex responsible for the movement of glutamate across the cell membrane working together with ions like sodium and potassium. Due to its significant role in the regulation of neurotransmitter levels in the brain EAAT2 is considered an important player in neural communication processes.

Pathways

EAAT2 is integral to the glutamatergic signaling pathway where its activity supports glutamate recycling and homeostasis. This pathway is important for normal cognitive functions such as learning and memory. EAAT2 works closely with other proteins such as EAAT1 in efforts to regulate glutamate concentrations in the extracellular space impacting synaptic strength and plasticity in the nervous system.

Associated diseases and disorders

Malfunctions of EAAT2 are associated with conditions like amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. In ALS EAAT2 dysfunction leads to accumulation of neurotoxic levels of glutamate causing motor neuron death. Similarly in Alzheimer’s disease impaired regulation of EAAT2 contributes to neurodegeneration through a related increase in neuronal excitotoxicity. Both conditions emphasize the critical importance of EAAT2 in neuroprotection and maintenance of normal brain function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-EAAT2 antibody (ab41621), expandable thumbnail

    Western blot - Anti-EAAT2 antibody (ab41621)

    All lanes: Western blot - Anti-EAAT2 antibody (ab41621) at 1 µg/mL

    Lane 1: Mouse brain tissue lysate at 10 µg

    Lane 2: Rat brain tissue lysate at 10 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 62 kDa

    Observed band size: 62 kDa

  • Immunocytochemistry - Anti-EAAT2 antibody (ab41621), expandable thumbnail

    Immunocytochemistry - Anti-EAAT2 antibody (ab41621)

    ab41621 staining EAAT2 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab41621 at 10μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 100% methanol (5 min).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Immunocytochemistry - Anti-EAAT2 antibody (ab41621), expandable thumbnail
    This image is courtesy of an anonymous customer review.

    Immunocytochemistry - Anti-EAAT2 antibody (ab41621)

    Methanol fixed human astrocytes (differentiated from H9-derived neuronal) cells labeling EAAT2 using ab41621 at a 1/250 dilution, 1 hr, 22°C (green) followed by an Alexa Fluor® 488 secondary antibody (1/1000 dilution), in ICC/IF.

    Cells were blocked in 5% BSA for 30 mins, 22°C.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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