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Rabbit Recombinant Monoclonal EAAT2 antibody. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Mouse, Rat samples. Cited in 9 publications.

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Images

Western blot - Anti-EAAT2 antibody [EPR19798] (AB205248), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] (AB205248), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] (AB205248), expandable thumbnail
  • Immunoprecipitation - Anti-EAAT2 antibody [EPR19798] (AB205248), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] (AB205248), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-FrIHC-P
Mouse
Tested
Tested
Tested
Tested
Rat
Expected
Tested
Expected
Tested

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

4 products for Alternative Product

1 product for Alternative Version

Target data

Function

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7557442, PubMed:7698742, PubMed:9373176). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (By similarity). Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate (PubMed:9180080).

Alternative names

Eaat2, Glt1, Slc1a2, Excitatory amino acid transporter 2, GLT-1, Sodium-dependent glutamate/aspartate transporter 2, Solute carrier family 1 member 2

Recommended products

Rabbit Recombinant Monoclonal EAAT2 antibody. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Mouse, Rat samples. Cited in 9 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19798
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

EAAT2 also known as excitatory amino acid transporter 2 or GLT-1 is a protein responsible for reuptake of glutamate from the synaptic cleft into glial cells preventing excitotoxicity. The protein has a mass of approximately 73 kDa and prominently expresses in the central nervous system particularly in astrocytes. By regulating extracellular glutamate levels EAAT2 helps maintain neurotransmitter balance critical for healthy neuron function.

Biological function summary

EAAT2 plays an important role in maintaining synaptic transmission and preventing overexcitation that can lead to neuronal damage. It is part of a transporter complex responsible for the movement of glutamate across the cell membrane working together with ions like sodium and potassium. Due to its significant role in the regulation of neurotransmitter levels in the brain EAAT2 is considered an important player in neural communication processes.

Pathways

EAAT2 is integral to the glutamatergic signaling pathway where its activity supports glutamate recycling and homeostasis. This pathway is important for normal cognitive functions such as learning and memory. EAAT2 works closely with other proteins such as EAAT1 in efforts to regulate glutamate concentrations in the extracellular space impacting synaptic strength and plasticity in the nervous system.

Associated diseases and disorders

Malfunctions of EAAT2 are associated with conditions like amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. In ALS EAAT2 dysfunction leads to accumulation of neurotoxic levels of glutamate causing motor neuron death. Similarly in Alzheimer’s disease impaired regulation of EAAT2 contributes to neurodegeneration through a related increase in neuronal excitotoxicity. Both conditions emphasize the critical importance of EAAT2 in neuroprotection and maintenance of normal brain function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-EAAT2 antibody [EPR19798] (ab205248), expandable thumbnail

    Western blot - Anti-EAAT2 antibody [EPR19798] (ab205248)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    1. EAAT2 is a multi-pass membrane protein, the main transporter that clears the excitatory neurotransmitter glutamate in the central nervous system. The bands around 180 kDa are multimers of EAAT2, which is consistent with what have been described in the literatures (PMID: 24569372 & 20193040).

    2. EATT2 is primarily expressed in astrocytes but is also expressed in neurons of the retina and during fetal development(PMID:12176072).

    All lanes: Western blot - Anti-EAAT2 antibody [EPR19798] (ab205248) at 1/2000 dilution

    Lane 1: Mouse brain lysate at 20 µg

    Lane 2: Mouse liver lysate at 20 µg

    Lane 3: Mouse kidney lysate at 20 µg

    Lane 4: Rat brain lysate at 20 µg

    Lane 5: Rat liver lysate at 20 µg

    Lane 6: Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg

    Lane 7: C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

    Lane 8: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

    Lane 9: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg

    Lane 10: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 62 kDa

    Observed band size: 180 kDa, 65 kDa

    Exposure time: 1s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] (ab205248), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] (ab205248)

    Immunohistochemical analysis of paraffin-embedded mouse striatum tissue labeling EAAT2 with ab205248 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Membrane staining on mouse striatum is observed [PMID 25391854]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] (ab205248), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] (ab205248)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling EAAT2 with ab205248 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Negative control: no staining on mouse kidney [PMID: 11038258].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-EAAT2 antibody [EPR19798] (ab205248), expandable thumbnail

    Immunoprecipitation - Anti-EAAT2 antibody [EPR19798] (ab205248)

    EAAT2 was immunoprecipitated from 0.35 mg of Mouse brain lysate with ab205248 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab205248 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse brain lysate, 10μg (Input).

    Lane 2: ab205248 IP in Mouse brain lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A]- Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab205248 in Mouse brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    All lanes: Immunoprecipitation - Anti-EAAT2 antibody [EPR19798] (ab205248)

    Predicted band size: 62 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] (ab205248), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] (ab205248)

    Immunohistochemical analysis of paraffin-embedded rat striatum tissue labeling EAAT2 with ab205248 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Membrane staining on Rat striatum is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-EAAT2 antibody [EPR19798] (ab205248), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-EAAT2 antibody [EPR19798] (ab205248)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse striatum tissue labeling EAAT2 with ab205248 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Membrane staining on mouse striatum is observed [PMID: 25391854]. The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.

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