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AB313454

Anti-EAAT2 antibody [RP23040136]

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(2 Publications)

Rabbit Recombinant Multiclonal EAAT2 antibody. Suitable for ICC/IF, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SLC1A2.

View Alternative Names

EAAT2, GLT1, SLC1A2, Excitatory amino acid transporter 2, Glutamate/aspartate transporter II, Sodium-dependent glutamate/aspartate transporter 2, Solute carrier family 1 member 2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-EAAT2 Antibody [RP23040136] (AB313454)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EAAT2 Antibody [RP23040136] (AB313454)

Immunofluorescence was performed on fixed and permeabilized U-87 MG cells for detection of endogenous EAAT2 using ab313454 (2 ug/ml) and labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (1 : 2000). Panel a) shows representative cells that were stained for detection and localization of EAAT2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (1 : 300). Panel d) is a composite image of Panels a, b and c clearly demonstrating membrane localization of EAAT2. Panel e) represents control cells with no primary antibody to assess background.

Western blot - Anti-EAAT2 Antibody [RP23040136] (AB313454)
  • WB

Supplier Data

Western blot - Anti-EAAT2 Antibody [RP23040136] (AB313454)

All lanes:

Western blot - Anti-EAAT2 Antibody [RP23040136] (ab313454) at 2 µg/mL

Lane 1:

Whole cell extracts of PC-12 at 30 µg

Lane 2:

Whole cell extracts of U-87 MG at 30 µg

Lane 3:

Whole cell extracts of SH-SY5Y at 30 µg

Lane 4:

Whole cell extracts of Neuro-2a at 30 µg

Lane 5:

Whole cell extracts of K-562 at 30 µg

Lane 6:

Whole cell extracts of HEL at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

true

Western blot - Anti-EAAT2 Antibody [RP23040136] (AB313454)
  • WB

Supplier Data

Western blot - Anti-EAAT2 Antibody [RP23040136] (AB313454)

All lanes:

Western blot - Anti-EAAT2 Antibody [RP23040136] (ab313454) at 2 µg/mL

Lane 1:

Mouse Brain at 30 µg

Lane 2:

Mouse Cerebellum at 30 µg

Lane 3:

Mouse Kidney at 30 µg

Lane 4:

Mouse Lung at 30 µg

Lane 5:

Rat Brain at 30 µg

Lane 6:

Rat Cerebellum at 30 µg

Lane 7:

Rat Kidney at 30 µg

Lane 8:

Rat Lung at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

true

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040136

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human SLC1A2.

P43004

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Primary Antibodies

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EAAT2 also known as excitatory amino acid transporter 2 or GLT-1 is a protein responsible for reuptake of glutamate from the synaptic cleft into glial cells preventing excitotoxicity. The protein has a mass of approximately 73 kDa and prominently expresses in the central nervous system particularly in astrocytes. By regulating extracellular glutamate levels EAAT2 helps maintain neurotransmitter balance critical for healthy neuron function.
Biological function summary

EAAT2 plays an important role in maintaining synaptic transmission and preventing overexcitation that can lead to neuronal damage. It is part of a transporter complex responsible for the movement of glutamate across the cell membrane working together with ions like sodium and potassium. Due to its significant role in the regulation of neurotransmitter levels in the brain EAAT2 is considered an important player in neural communication processes.

Pathways

EAAT2 is integral to the glutamatergic signaling pathway where its activity supports glutamate recycling and homeostasis. This pathway is important for normal cognitive functions such as learning and memory. EAAT2 works closely with other proteins such as EAAT1 in efforts to regulate glutamate concentrations in the extracellular space impacting synaptic strength and plasticity in the nervous system.

Malfunctions of EAAT2 are associated with conditions like amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. In ALS EAAT2 dysfunction leads to accumulation of neurotoxic levels of glutamate causing motor neuron death. Similarly in Alzheimer’s disease impaired regulation of EAAT2 contributes to neurodegeneration through a related increase in neuronal excitotoxicity. Both conditions emphasize the critical importance of EAAT2 in neuroprotection and maintenance of normal brain function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed : 14506254, PubMed : 15265858, PubMed : 26690923, PubMed : 7521911). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (PubMed : 14506254). Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (PubMed : 14506254). Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate (By similarity).
See full target information SLC1A2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell & bioscience 15:124 PubMed40877964

2025

Enriched environment inhibits GLT-1 ubiquitination by downregulating SMURF1 to attenuate ischemic brain injury induced excitotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhang,Wen Liu,Tao Huang,Lingling Liu,Xiuping Chen

BMC biology 23:86 PubMed40140835

2025

Ectopic expression of Slc1a2 in the prefrontal cortex of sleep-deprived male mice counteracts the glutamate/GABA-glutamine dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Fengying Zhang,Yao Li,Li Jiang,Yingbin Wang,Yonghong Tang,Bo Ouyang,Guojun Song,Xuan Li,Xiajie Quan,Min Li,Hongying Wang,Zhao Pan,Keyan Chen,Ping Zhang
View all publications
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Product promise

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