Anti-EAAT3 antibody [EPR25149-62] (ab288441)

Rabbit Recombinant Monoclonal EAAT3 antibody. Suitable for IP, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Rat samples.

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Images

Western blot - Anti-EAAT3 antibody [EPR25149-62] (AB288441), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	IHC-P	ICC/IF	IHC-Fr	Flow Cyt (Intra)
Human	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Tested	Tested	Not recommended
Rat	Tested	Tested	Tested	Not recommended	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes -
Species Rat	Dilution info 1/30	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/50	Notes -
Species Mouse	Dilution info 1/50	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Target data

See full target information Slc1a1

Function

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:12119102, PubMed:18684713). Can also transport L-cysteine (PubMed:30840898). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (By similarity). Plays an important role in L-glutamate and L-aspartate reabsorption in renal tubuli (PubMed:9233792). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (PubMed:9233792). Contributes to glutathione biosynthesis and protection against oxidative stress via its role in L-glutamate and L-cysteine transport (PubMed:30840898). Negatively regulated by ARL6IP5 (PubMed:12119102).

Alternative names

Eaac1, Eaat3, Slc1a1, Excitatory amino acid transporter 3, Excitatory amino-acid carrier 1, Sodium-dependent glutamate/aspartate transporter 3, Solute carrier family 1 member 1

Recommended products

Rabbit Recombinant Monoclonal EAAT3 antibody. Suitable for IP, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR25149-62
Purification technique: Affinity purification Protein A
Specificity: ICC application does not react with Rat species
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

EAAT3 also known as Excitatory Amino Acid Transporter 3 or SLC1A1 is a protein that transports amino acids particularly glutamate across cellular membranes. EAAT3 has a mass of approximately 57 kDa. It is expressed in various tissues including the brain and kidneys. In neurons it accumulates glutamate into the cells which is important for maintaining synaptic transmission balance and preventing excitotoxic damage.

Biological function summary

EAAT3 functions to regulate extracellular levels of glutamate an essential neurotransmitter. The transporter prevents excessive accumulation of glutamate in the synaptic cleft promoting synaptic efficiency and neuron communication. EAAT3 operates as part of a larger family of transporters including EAAT1 EAAT2 and others that collectively maintain glutamate homeostasis in the central nervous system.

Pathways

EAAT3 engages in neurotransmitter metabolism and the glutamatergic synapse. It collaborates with EAAT2 in similar pathways. Together they ensure that excess glutamate is absorbed and reused or degraded safeguarding against excitotoxicity. These pathways are critical for maintaining neuronal health and function.

Associated diseases and disorders

EAAT3 has links to obsessive-compulsive disorder (OCD) and epilepsy. EAAT3 dysfunction or altered expression can lead to imbalance in neurotransmitter levels contributing to these conditions. Changes in EAAT2 a related transporter also have implications in these disorders. Understanding these interactions helps in exploring EAAT3 as a possible therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

16 product images

Western blot - Anti-EAAT3 antibody [EPR25149-62] (ab288441)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: Lanes 1-4: 26 seconds; Lanes 5-7: 3 minutes
Monomers: 50-75 kda; Aggregates: 150-250 kda
The MW and expression pattern is consistent to the literature (PMID: 30840898)
All lanes: Western blot - Anti-EAAT3 antibody [EPR25149-62] (ab288441) at 1/1000 dilution
Lane 1: Mouse kidney tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Mouse spleen tissue lysate at 20 µg
Lane 4: Mouse testis tissue lysate at 20 µg
Lane 5: Rat hippocampus tissue lysate at 20 µg
Lane 6: Rat brain tissue lysate at 20 µg
Lane 7: Rat testis tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 150-250 kDa, 50-75 kDa
Immunohistochemistry (Frozen sections) - Anti-EAAT3 antibody [EPR25149-62] (ab288441)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney (fresh) tissue labeling EAAT3 with ab288441 at 1/500 (0.982 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Positive staining on the proximal convoluted tubule of rat kidney is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
Immunohistochemistry (Frozen sections) - Anti-EAAT3 antibody [EPR25149-62] (ab288441)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney (fresh) tissue labeling EAAT3 with ab288441 at 1/500 (0.982 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Positive staining on the proximal convoluted tubule of mouse kidney is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
Immunoprecipitation - Anti-EAAT3 antibody [EPR25149-62] (ab288441)
EAAT3 was immunoprecipitated from 0.35 mg Rat hippocampus tissue lysate 10 ug with ab288441 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab288441 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Rat hippocampus tissue lysate 10 ug
Lane 2: ab288441 IP in Rat hippocampus tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab288441 in rat hippocampus tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds
All lanes: Immunoprecipitation - Anti-EAAT3 antibody [EPR25149-62] (ab288441)
Predicted band size: 57 kDa
Immunohistochemistry (Frozen sections) - Anti-EAAT3 antibody [EPR25149-62] (ab288441)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) (-) tissue labeling EAAT3 with ab288441 at 1/50 (9.82 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). No staining on rat liver (Negative control: PMID 11242046) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
Immunoprecipitation - Anti-EAAT3 antibody [EPR25149-62] (ab288441)
EAAT3 was immunoprecipitated from 0.35 mg Mouse hippocampus tissue lysate 10 ug with ab288441 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab288441 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse hippocampus tissue lysate 10 ug
Lane 2: ab288441 IP in Mouse hippocampus tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab288441 in mouse hippocampus tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds
All lanes: Immunoprecipitation - Anti-EAAT3 antibody [EPR25149-62] (ab288441)
Predicted band size: 57 kDa
Immunohistochemistry (Frozen sections) - Anti-EAAT3 antibody [EPR25149-62] (ab288441)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (fresh) tissue labeling EAAT3 with ab288441 at 1/50 (9.82 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Positive staining on rat hippocampus is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
Immunohistochemistry (Frozen sections) - Anti-EAAT3 antibody [EPR25149-62] (ab288441)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) (-) tissue labeling EAAT3 with ab288441 at 1/50 (9.82 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). No staining on mouse liver (Negative control: PMID 11242046) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
Immunohistochemistry (Frozen sections) - Anti-EAAT3 antibody [EPR25149-62] (ab288441)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh) tissue labeling EAAT3 with ab288441 at 1/50 (9.82 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Positive staining on mouse cerebellum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
Immunocytochemistry/ Immunofluorescence - Anti-EAAT3 antibody [EPR25149-62] (ab288441)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling EAAT3 with ab288441 at 1/100 (4.91 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron cells. Confocal scanning Z step was set as 0.3 um followed by image processing with maximum Z projection. Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain neuron-specific cytoskeletal protein at 1/500 (4ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2ug/ml) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT3 antibody [EPR25149-62] (ab288441)
Immunohistochemical analysis of paraffin-embedded Rat testis tissue labelling EAAT3 with ab288441 at 1/1000 (0.491 ug/ml) followed by a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used. Negative control: no staining on rat testis.The section was incubated with ab288441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT3 antibody [EPR25149-62] (ab288441)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling EAAT3 with ab288441 at 1/1000 (0.491 ug/ml) followed by a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used. Negative control: no staining on mouse spleen.The section was incubated with ab288441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT3 antibody [EPR25149-62] (ab288441)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling EAAT3 with ab288441 at 1/1000 (0.491 ug/ml) followed by a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used. Cytoplasmic staining on rat cerebrum (PMID: 11242046). The section was incubated with ab288441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT3 antibody [EPR25149-62] (ab288441)
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labelling EAAT3 with ab288441 at 1/1000 (0.491 ug/ml) followed by a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used. Apical staining on proximal convoluted tubules in rat kidney (PMID: 9435692).The section was incubated with ab288441 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT3 antibody [EPR25149-62] (ab288441)
Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labelling EAAT3 with ab288441 at 1/1000 (0.491 ug/ml) followed by a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used. Cytoplasmic staining on mouse hippocampus (PMID: 11242046).The section was incubated with ab288441 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT3 antibody [EPR25149-62] (ab288441)
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labelling EAAT3 with ab288441 at 1/1000 (0.491 ug/ml) followed by a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used. Apical staining on proximal convoluted tubules in mouse kidney (PMID: 9435692).The section was incubated with ab288441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins