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AB124802

Anti-EAAT3 antibody [EPR6774(B)]

  • 20ul selling size
  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • What is this?

4

(3 Reviews)

|

(19 Publications)

Rabbit Recombinant Monoclonal EAAT3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 19 publications.

View Alternative Names

EAAC1, EAAT3, HEAAC1, SLC1A1, Excitatory amino acid transporter 3, Excitatory amino-acid carrier 1, Neuronal and epithelial glutamate transporter, Sodium-dependent glutamate/aspartate transporter 3, Solute carrier family 1 member 1

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT3 antibody [EPR6774(B)] (AB124802)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT3 antibody [EPR6774(B)] (AB124802)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue sections labeling EAAT3 with purified ab124802 at 1/1000 dilution (0.8 μg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT3 antibody [EPR6774(B)] (AB124802)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT3 antibody [EPR6774(B)] (AB124802)

Unpurified ab124802, at 1/500 dilution, staining EAAT3 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-EAAT3 antibody [EPR6774(B)] (AB124802)
  • WB

Lab

Western blot - Anti-EAAT3 antibody [EPR6774(B)] (AB124802)

Blocking and Diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-EAAT3 antibody [EPR6774(B)] (ab124802) at 1/50000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

Human testis tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 57 kDa

Observed band size: 70 kDa

false

Exposure time: 30s

Western blot - Anti-EAAT3 antibody [EPR6774(B)] (AB124802)
  • WB

Lab

Western blot - Anti-EAAT3 antibody [EPR6774(B)] (AB124802)

We are unsure how to define the extra bands.

All lanes:

Western blot - Anti-EAAT3 antibody [EPR6774(B)] (ab124802) at 1/1000 dilution

All lanes:

Human brain lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 57 kDa

Observed band size: 70 kDa

false

OI-RD Scanning - Anti-EAAT3 antibody [EPR6774(B)] (AB124802)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-EAAT3 antibody [EPR6774(B)] (AB124802)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-EAAT3 antibody [EPR6774(B)] - BSA and Azide free

  • HRP

    HRP Anti-EAAT3 antibody [EPR6774(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6774(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p>Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EAAT3 also known as Excitatory Amino Acid Transporter 3 or SLC1A1 is a protein that transports amino acids particularly glutamate across cellular membranes. EAAT3 has a mass of approximately 57 kDa. It is expressed in various tissues including the brain and kidneys. In neurons it accumulates glutamate into the cells which is important for maintaining synaptic transmission balance and preventing excitotoxic damage.
Biological function summary

EAAT3 functions to regulate extracellular levels of glutamate an essential neurotransmitter. The transporter prevents excessive accumulation of glutamate in the synaptic cleft promoting synaptic efficiency and neuron communication. EAAT3 operates as part of a larger family of transporters including EAAT1 EAAT2 and others that collectively maintain glutamate homeostasis in the central nervous system.

Pathways

EAAT3 engages in neurotransmitter metabolism and the glutamatergic synapse. It collaborates with EAAT2 in similar pathways. Together they ensure that excess glutamate is absorbed and reused or degraded safeguarding against excitotoxicity. These pathways are critical for maintaining neuronal health and function.

EAAT3 has links to obsessive-compulsive disorder (OCD) and epilepsy. EAAT3 dysfunction or altered expression can lead to imbalance in neurotransmitter levels contributing to these conditions. Changes in EAAT2 a related transporter also have implications in these disorders. Understanding these interactions helps in exploring EAAT3 as a possible therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed : 21123949, PubMed : 26690923, PubMed : 33658209, PubMed : 7521911, PubMed : 7914198, PubMed : 8857541). Can also transport L-cysteine (PubMed : 21123949). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (PubMed : 26690923, PubMed : 33658209, PubMed : 7521911, PubMed : 8857541). Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (PubMed : 26690923, PubMed : 8857541). Plays an important role in L-glutamate and L-aspartate reabsorption in renal tubuli (PubMed : 21123949). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity). Contributes to glutathione biosynthesis and protection against oxidative stress via its role in L-glutamate and L-cysteine transport (By similarity). Negatively regulated by ARL6IP5 (By similarity).
See full target information SLC1A1
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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Archives of toxicology 97:2371-2383 PubMed37482551

2023

Chronic exposure to inorganic arsenic and fluoride induces redox imbalance, inhibits the transsulfuration pathway, and alters glutamate receptor expression in the brain, resulting in memory impairment in adult male mouse offspring.

Applications

Unspecified application

Species

Unspecified reactive species

Wendy L González-Alfonso,Petrosyan Pavel,Hernández-Mercado Karina,Luz M Del Razo,Luz C Sanchez-Peña,Angélica Zepeda,María E Gonsebatt

EBioMedicine 72:103614 PubMed34628354

2021

SLC1A1 mediated glutamine addiction and contributed to natural killer T-cell lymphoma progression with immunotherapeutic potential.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Xiong,Nan Wang,Hui-Juan Zhong,Bo-Wen Cui,Shu Cheng,Rui Sun,Jia-Yi Chen,Peng-Peng Xu,Gang Cai,Li Wang,Xiao-Jian Sun,Jin-Yan Huang,Wei-Li Zhao

Cancers 13: PubMed33801101

2021

A Cystine-Cysteine Intercellular Shuttle Prevents Ferroptosis in xCT Pancreatic Ductal Adenocarcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Willian Meira,Boutaina Daher,Scott Kenneth Parks,Yann Cormerais,Jerome Durivault,Eric Tambutte,Jacques Pouyssegur,Milica Vučetić

Communications biology 4:182 PubMed33568779

2021

Inhibition of miR-96-5p in the mouse brain increases glutathione levels by altering NOVA1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Chisato Kinoshita,Kazue Kikuchi-Utsumi,Koji Aoyama,Ryo Suzuki,Yayoi Okamoto,Nobuko Matsumura,Daiki Omata,Kazuo Maruyama,Toshio Nakaki

International journal of molecular sciences 21: PubMed33256007

2020

Early Postnatal Exposure to a Low Dose of Nanoparticulate Silver Induces Alterations in Glutamate Transporters in Brain of Immature Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Beata Dąbrowska-Bouta,Grzegorz Sulkowski,Mikołaj Sałek,Magdalena Gewartowska,Marta Sidoryk-Węgrzynowicz,Lidia Strużyńska

The British journal of nutrition :1-9 PubMed33143768

2020

Gallic acid affects intestinal-epithelial-cell integrity and selected amino-acid uptake in porcine and permeability models.

Applications

Unspecified application

Species

Unspecified reactive species

Marco Tretola,Giuseppe Bee,Paolo Silacci

Neurochemistry international 141:104888 PubMed33199267

2020

Sexually dimorphic and brain region-specific transporter adaptations in system x null mice.

Applications

Unspecified application

Species

Unspecified reactive species

Heather M Sosnoski,Sheila M S Sears,Yan He,Carla Frare,Sandra J Hewett

Journal of animal science 98: PubMed32564078

2020

The effects of maternal nutrient restriction and day of early pregnancy on the location and abundance of neutral amino acid transporters in beef heifer utero-placental tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew S Crouse,Kyle J McLean,Nathaniel P Greseth,Alison K Ward,Lawrence P Reynolds,Carl R Dahlen,Bryan W Neville,Pawel P Borowicz,Joel S Caton

Frontiers in cellular neuroscience 14:17 PubMed32194376

2020

Early Neurotoxic Effects of Inorganic Arsenic Modulate Cortical GSH Levels Associated With the Activation of the Nrf2 and NFκB Pathways, Expression of Amino Acid Transporters and NMDA Receptors and the Production of Hydrogen Sulfide.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela Silva-Adaya,Lucio Antonio Ramos-Chávez,Pavel Petrosyan,Wendy Leslie González-Alfonso,Alegna Pérez-Acosta,Maria E Gonsebatt

The Journal of experimental medicine 217: PubMed31699822

2019

Endothelial Cdk5 deficit leads to the development of spontaneous epilepsy through CXCL1/CXCR2-mediated reactive astrogliosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiu-Xiu Liu,Lin Yang,Ling-Xiao Shao,Yang He,Gang Wu,Yu-Huan Bao,Nan-Nan Lu,Dong-Mei Gong,Ya-Ping Lu,Tian-Tian Cui,Ning-He Sun,Dan-Yang Chen,Wei-Xing Shi,Kohji Fukunaga,Hong-Shan Chen,Zhong Chen,Feng Han,Ying-Mei Lu
View all publications
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Product promise

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