Rabbit Polyclonal EAAT3 antibody. N-terminal. Suitable for WB and reacts with Rat, Mouse samples. Immunogen corresponding to Synthetic Peptide within Mouse Slc1a1 aa 1-50.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium dihydrogen phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Mouse Slc1a1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P51906
Reactivity data
Select an application| WB | |
|---|---|
| Mouse | Tested |
| Rat | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.10000-0.50000 µg/mL | Notes - |
Species Mouse | Dilution info 0.10000-0.50000 µg/mL | Notes - |
Associated Products
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Target data
Function
Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:12119102, PubMed:18684713). Can also transport L-cysteine (PubMed:30840898). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (By similarity). Plays an important role in L-glutamate and L-aspartate reabsorption in renal tubuli (PubMed:9233792). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (PubMed:9233792). Contributes to glutathione biosynthesis and protection against oxidative stress via its role in L-glutamate and L-cysteine transport (PubMed:30840898). Negatively regulated by ARL6IP5 (PubMed:12119102).
Alternative names
Eaac1, Eaat3, Slc1a1, Excitatory amino acid transporter 3, Excitatory amino-acid carrier 1, Sodium-dependent glutamate/aspartate transporter 3, Solute carrier family 1 member 1
Recommended products
Rabbit Polyclonal EAAT3 antibody. N-terminal. Suitable for WB and reacts with Rat, Mouse samples. Immunogen corresponding to Synthetic Peptide within Mouse Slc1a1 aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium dihydrogen phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Mouse Slc1a1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P51906
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EAAT3 also known as Excitatory Amino Acid Transporter 3 or SLC1A1 is a protein that transports amino acids particularly glutamate across cellular membranes. EAAT3 has a mass of approximately 57 kDa. It is expressed in various tissues including the brain and kidneys. In neurons it accumulates glutamate into the cells which is important for maintaining synaptic transmission balance and preventing excitotoxic damage.
- Biological function summary
EAAT3 functions to regulate extracellular levels of glutamate an essential neurotransmitter. The transporter prevents excessive accumulation of glutamate in the synaptic cleft promoting synaptic efficiency and neuron communication. EAAT3 operates as part of a larger family of transporters including EAAT1 EAAT2 and others that collectively maintain glutamate homeostasis in the central nervous system.
- Pathways
EAAT3 engages in neurotransmitter metabolism and the glutamatergic synapse. It collaborates with EAAT2 in similar pathways. Together they ensure that excess glutamate is absorbed and reused or degraded safeguarding against excitotoxicity. These pathways are critical for maintaining neuronal health and function.
- Associated diseases and disorders
EAAT3 has links to obsessive-compulsive disorder (OCD) and epilepsy. EAAT3 dysfunction or altered expression can lead to imbalance in neurotransmitter levels contributing to these conditions. Changes in EAAT2 a related transporter also have implications in these disorders. Understanding these interactions helps in exploring EAAT3 as a possible therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-EAAT3 antibody - N-terminal (ab191290)
All lanes: Western blot - Anti-EAAT3 antibody - N-terminal (ab191290) at 0.5 µg/mL
Lane 1: Rat liver tissue lysate
Lane 2: Rat heart tissue lysate
Lane 3: HEPA cell lysate
Developed using the ECL technique.
Predicted band size: 57 kDa
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