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AB250887

Anti-EAAT4 antibody [EPR7742] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal EAAT4 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

EAAT4, SLC1A6, Excitatory amino acid transporter 4, Sodium-dependent glutamate/aspartate transporter, Solute carrier family 1 member 6

2 Images
Western blot - Anti-EAAT4 antibody [EPR7742] - BSA and Azide free (AB250887)
  • WB

Supplier Data

Western blot - Anti-EAAT4 antibody [EPR7742] - BSA and Azide free (AB250887)

This data was developed using ab186435, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EAAT4 antibody [EPR7742] - N-terminal (<a href='/en-us/products/primary-antibodies/eaat4-antibody-epr7742-n-terminal-ab186435'>ab186435</a>) at 1/1000 dilution

Lane 1:

C6 lysate at 10 µg

Lane 2:

Raw264.7 cell lysate at 10 µg

Lane 3:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 62 kDa

Observed band size: 62 kDa

false

Western blot - Anti-EAAT4 antibody [EPR7742] - BSA and Azide free (AB250887)
  • WB

Supplier Data

Western blot - Anti-EAAT4 antibody [EPR7742] - BSA and Azide free (AB250887)

This data was developed using ab186435, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EAAT4 antibody [EPR7742] - N-terminal (<a href='/en-us/products/primary-antibodies/eaat4-antibody-epr7742-n-terminal-ab186435'>ab186435</a>) at 1/10000 dilution

Lane 1:

Human astrocytoma lysate at 20 µg

Lane 2:

U87-MG cell lysate at 20 µg

Lane 3:

A375 cell lysate at 20 µg

Lane 4:

PC3 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 62 kDa

Observed band size: 62 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7742

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250887 is the carrier-free version of ab186435.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EAAT4 also known as Excitatory Amino Acid Transporter 4 or SLC1A6 functions as a sodium-dependent glutamate transporter primarily facilitating the reuptake of glutamate into presynaptic neurons and glial cells. EAAT4 has an approximate molecular mass of 65 kDa. Expression occurs mainly in Purkinje cells within the cerebellum but it can also be found in other parts of the central nervous system albeit at lower levels.
Biological function summary

EAAT4 controls extracellular glutamate concentration preventing excitotoxicity in neural circuits. EAAT4 forms part of the larger family of high-affinity glutamate transporters which includes EAAT1 to EAAT5. This transporter operates by coupling glutamate transport with the influx of sodium and the efflux of potassium and protons thereby maintaining ion balance. Although single in its action EAAT4 works alongside related transporters for comprehensive glutamate regulation.

Pathways

EAAT4 plays a significant role in glutamatergic signaling and cerebellar function. The transporter deeply integrates into the synaptic transmission pathway ensuring glutamate homeostasis critical for synaptic plasticity. EAAT4 works in functional tandem with proteins like EAAT3 facilitating efficient glutamate clearance and modulating excitatory signaling within neuronal pathways.

EAAT4 relates to neurodegenerative conditions where disrupted glutamate homeostasis plays a part such as Amyotrophic Lateral Sclerosis (ALS) and Spinocerebellar Ataxia. Excessive extracellular glutamate can lead to neuronal damage linking EAAT4 function with these pathologies. Moreover EAAT4 interacts with proteins like GLT-1 in these disease contexts to attempt to mitigate glutamatergic dysfunction.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed : 26690923, PubMed : 7791878). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (PubMed : 7791878). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (Probable).
See full target information SLC1A6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of radiation research 64:833-841 PubMed37439405

2023

Expression of TMEM59L associated with radiosensitive in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Gao,Peng Wang,Lin Zhi,Shibin Sun,Xiaoguang Qiu,Yanwei Liu
View all publications
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Product promise

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