JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB186435

Anti-EAAT4 antibody [EPR7742] - N-terminal

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Recombinant Monoclonal EAAT4 antibody. N-terminal. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

View Alternative Names

EAAT4, SLC1A6, Excitatory amino acid transporter 4, Sodium-dependent glutamate/aspartate transporter, Solute carrier family 1 member 6

2 Images
Western blot - Anti-EAAT4 antibody [EPR7742] - N-terminal (AB186435)
  • WB

Supplier Data

Western blot - Anti-EAAT4 antibody [EPR7742] - N-terminal (AB186435)

All lanes:

Western blot - Anti-EAAT4 antibody [EPR7742] - N-terminal (ab186435) at 1/10000 dilution

Lane 1:

Human astrocytoma lysate at 20 µg

Lane 2:

U87-MG cell lysate at 20 µg

Lane 3:

A375 cell lysate at 20 µg

Lane 4:

PC3 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 62 kDa

Observed band size: 62 kDa

false

Western blot - Anti-EAAT4 antibody [EPR7742] - N-terminal (AB186435)
  • WB

Supplier Data

Western blot - Anti-EAAT4 antibody [EPR7742] - N-terminal (AB186435)

All lanes:

Western blot - Anti-EAAT4 antibody [EPR7742] - N-terminal (ab186435) at 1/1000 dilution

Lane 1:

C6 lysate at 10 µg

Lane 2:

Raw264.7 cell lysate at 10 µg

Lane 3:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 62 kDa

Observed band size: 62 kDa

false

  • Carrier free

    Anti-EAAT4 antibody [EPR7742] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7742

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB138492

Anti-Collagen I antibody [EPR7785]

20ul selling size|BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [EPR7785] (AB138492)

  • 4
  • 37
Primary Antibodies

AB34710

Anti-Collagen I + Collagen III antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I + Collagen III antibody (AB34710)

  • 5
  • 91
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 16
Primary Antibodies

AB268020

Anti-Fibronectin antibody [EPR23110-46]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-46] (AB268020)

  • 4
  • 5
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EAAT4 also known as Excitatory Amino Acid Transporter 4 or SLC1A6 functions as a sodium-dependent glutamate transporter primarily facilitating the reuptake of glutamate into presynaptic neurons and glial cells. EAAT4 has an approximate molecular mass of 65 kDa. Expression occurs mainly in Purkinje cells within the cerebellum but it can also be found in other parts of the central nervous system albeit at lower levels.
Biological function summary

EAAT4 controls extracellular glutamate concentration preventing excitotoxicity in neural circuits. EAAT4 forms part of the larger family of high-affinity glutamate transporters which includes EAAT1 to EAAT5. This transporter operates by coupling glutamate transport with the influx of sodium and the efflux of potassium and protons thereby maintaining ion balance. Although single in its action EAAT4 works alongside related transporters for comprehensive glutamate regulation.

Pathways

EAAT4 plays a significant role in glutamatergic signaling and cerebellar function. The transporter deeply integrates into the synaptic transmission pathway ensuring glutamate homeostasis critical for synaptic plasticity. EAAT4 works in functional tandem with proteins like EAAT3 facilitating efficient glutamate clearance and modulating excitatory signaling within neuronal pathways.

EAAT4 relates to neurodegenerative conditions where disrupted glutamate homeostasis plays a part such as Amyotrophic Lateral Sclerosis (ALS) and Spinocerebellar Ataxia. Excessive extracellular glutamate can lead to neuronal damage linking EAAT4 function with these pathologies. Moreover EAAT4 interacts with proteins like GLT-1 in these disease contexts to attempt to mitigate glutamatergic dysfunction.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed : 26690923, PubMed : 7791878). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (PubMed : 7791878). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (Probable).
See full target information SLC1A6
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancers 13: PubMed33801101

2021

A Cystine-Cysteine Intercellular Shuttle Prevents Ferroptosis in xCT Pancreatic Ductal Adenocarcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Willian Meira,Boutaina Daher,Scott Kenneth Parks,Yann Cormerais,Jerome Durivault,Eric Tambutte,Jacques Pouyssegur,Milica Vučetić

Biology of reproduction 99:789-797 PubMed29733335

2018

The flagellar protein Enkurin is required for mouse sperm motility and for transport through the female reproductive tract.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa K Jungnickel,Keith A Sutton,Mark A Baker,Michael G Cohen,Michael J Sanderson,Harvey M Florman
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com