AB237753

Anti-EAF2 antibody

Name: Anti-EAF2 antibody (ab237753) | Abcam
Brand: Abcam Limited
SKU: AB237753
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal EAF2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EAF2 aa 1-100.

View Alternative Names

TRAITS, BM-040, EAF2, ELL-associated factor 2, Testosterone-regulated apoptosis inducer and tumor suppressor protein

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAF2 antibody (AB237753)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAF2 antibody (AB237753)

Paraffin-embedded human appendix tissue stained for EAF2 using ab237753 at 1/1,000 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

Immunocytochemistry/ Immunofluorescence - Anti-EAF2 antibody (AB237753)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EAF2 antibody (AB237753)

HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for EAF2 (green) using ab237753 at 1/100 dilution in ICC/IF, followed by an Alexa-Fluor^® 488 conjugated Goat Anti-Rabbit IgG (H+L).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human EAF2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q96CJ1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity greater than 95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EAF2 also known as ELL-associated factor 2 plays an important role in transcription regulation. This protein weighing approximately 32 kDa interacts with the ELL (eleven-nineteen lysine-rich leukemia) family of RNA polymerase II elongation factors. EAF2 expression is noted in various tissues including the prostate colon and liver indicating a broad impact on cellular functions across the body. Researchers often study EAF2 for its regulatory abilities and its influence on gene expression.

Biological function summary

EAF2 regulates the transcription elongation process. It works as part of a complex with ELL proteins impacting the rate at which RNA polymerase II can transcribe genetic information into messenger RNA. The EAF2-ELL complex stabilizes and enhances the transcription process ensuring proper gene expression and cellular function. Its influence on transcription shapes how cells respond to their environment making EAF2 a protein of interest in many studies.

Pathways

EAF2 significantly impacts the androgen receptor signaling pathway and transcriptional regulation pathways. In the androgen receptor pathway EAF2 influences gene expression by modulating the activity of this receptor affecting processes such as cell growth and differentiation. Furthermore it interacts with other proteins within these pathways including androgen receptor itself and transcriptional machinery components like RNA polymerase II linking it to cellular proliferation and hormone response.

EAF2 has a connection to prostate cancer and chronic liver disease. Its role in prostate cancer stems from its involvement in androgen receptor pathways where aberrant expression or dysfunction might contribute to tumor development and progression. EAF2 interacts with other proteins like ELL and androgen receptors making it a potential target for cancer therapy. In chronic liver disease understanding EAF2's contributions to transcription regulation might reveal its role in liver physiology and pathology providing insights into disease mechanisms and potential treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Acts as a transcriptional transactivator of TCEA1 elongation activity (By similarity). Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo.

See full target information EAF2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:178 PubMed39905234

2025

The PDE4DIP-AKAP9 axis promotes lung cancer growth through modulation of PKA signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Yangyang Fu,Shishun Huang,Rulu Pan,Xingan Chen,Ting Liu,Rongzhe Zhang,Fangsheng Zhu,Qiwei Fang,Liyue Wu,Juji Dai,Ouchen Wang,Liting Lu,Xiduan Wei,Liangxing Wang,Xincheng Lu

World journal of gastroenterology 29:3770-3792 PubMed37426316

2023

Mechanism of ELL-associated factor 2 and vasohibin 1 regulating invasion, migration, and angiogenesis in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Liang Feng,Ming-Jun Sun,Bo-Yang Xu,Meng-Yuan Liu,Hui-Jing Zhang,Can Wu

World journal of gastrointestinal oncology 14:1949-1967 PubMed36310706

2022

Overexpression of ELL-associated factor 2 suppresses invasion, migration, and angiogenesis in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Liang Feng,Can Wu,Hui-Jing Zhang,Huan Zhou,Tai-Wei Jiao,Meng-Yuan Liu,Ming-Jun Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-EAF2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAF2 antibody (AB237753)

Immunocytochemistry/ Immunofluorescence - Anti-EAF2 antibody (AB237753)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise