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AB247687

Anti-EAG1 antibody [EPR3802] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal EAG1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

EAG, EAG1, KCNH1, Voltage-gated delayed rectifier potassium channel KCNH1, Ether-a-go-go potassium channel 1, Potassium voltage-gated channel subfamily H member 1, Voltage-gated potassium channel subunit Kv10.1, EAG channel 1, h-eag, hEAG1

1 Images
Western blot - Anti-EAG1 antibody [EPR3802] - BSA and Azide free (AB247687)
  • WB

Unknown

Western blot - Anti-EAG1 antibody [EPR3802] - BSA and Azide free (AB247687)

This data was developed using ab108537, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EAG1 antibody [EPR3802] (<a href='/en-us/products/primary-antibodies/eag1-antibody-epr3802-ab108537'>ab108537</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

A673 cell lysate at 10 µg

Predicted band size: 111 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3802

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab247687 is the carrier-free version of ab108537.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EAG1 also known as Kv10.1 is a voltage-gated potassium channel with a molecular mass approximately 110 kDa. This protein plays a critical role in regulating membrane potential by allowing potassium ions to flow in response to voltage changes across the cell membrane. EAG1 is prominently expressed in the brain with presence noted in other tissues such as the heart and peripheral nervous system. Its activity influences the electrical excitability of neurons and has implications in cellular signaling.
Biological function summary

EAG1 is involved in key processes governing cellular excitability and proliferation. The channel contributes to setting the electrical properties of cells especially in neurons by controlling action potential dynamics. EAG1 does not typically form part of a complex but it can interact with other membrane proteins or signaling molecules modulating its activity. In certain contexts its aberrant expression can lead to altered cellular behaviors linking it to various cellular functional states.

Pathways

EAG1 engages in the regulation of the MAPK signaling pathway and is also significant in the PI3K/AKT pathway. These pathways are important for transmitting cellular signals that influence cell growth and survival. In the context of the MAPK pathway EAG1’s activity may interact with proteins such as B-Raf affecting downstream signaling events. Similarly in the PI3K/AKT pathway interactions can occur with proteins like AKT further integrating EAG1 within these essential frameworks.

EAG1 shows a connection to cancer and epilepsy. EAG1 overexpression has been documented in various cancers including breast and colon cancers where it may drive tumorigenic processes through its influence on cell proliferation and survival pathways. Moreover aberrant EAG1 function links to epilepsy potentially through its impact on neuronal excitability and neurotransmission. In these conditions EAG1 may interact with proteins such as p53 in cancer altering cell cycle control or with neurotransmitter receptors in epilepsy impacting neuronal networks.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pore-forming (alpha) subunit of a voltage-gated delayed rectifier potassium channel that mediates outward-rectifying potassium currents which, on depolarization, reaches a steady-state level and do not inactivate (PubMed : 10880439, PubMed : 11943152, PubMed : 22732247, PubMed : 25420144, PubMed : 25556795, PubMed : 25915598, PubMed : 27005320, PubMed : 27325704, PubMed : 27618660, PubMed : 30149017, PubMed : 9738473). The activation kinetics depend on the prepulse potential and external divalent cation concentration (PubMed : 11943152). With negative prepulses, the current activation is delayed and slowed down several fold, whereas more positive prepulses speed up activation (PubMed : 11943152). The time course of activation is biphasic with a fast and a slowly activating current component (PubMed : 11943152). Activates at more positive membrane potentials and exhibit a steeper activation curve (PubMed : 11943152). Channel properties are modulated by subunit assembly (PubMed : 11943152). Mediates IK(NI) current in myoblasts (PubMed : 9738473). Involved in the regulation of cell proliferation and differentiation, in particular adipogenic and osteogenic differentiation in bone marrow-derived mesenchymal stem cells (MSCs) (PubMed : 23881642).
See full target information KCNH1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:409 PubMed37600337

2023

Renal epithelioid angiomyolipoma: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyun Li,Xuebei Zhang,Qingli Zhao,Jin Wang,Xiaoqing Yang,Shengliang Huang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com