JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB201327

Anti-Eagle antibody [8B11DH10]

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Monoclonal Eagle antibody. Suitable for IHC (PFA fixed) and reacts with Drosophila melanogaster samples. Cited in 1 publication.

View Alternative Names

egon, NR0A3, spry, CG7383, eg, Protein embryonic gonad, Nuclear receptor subfamily 0 group A member 3, Protein eagle, Protein spready

1 Images
Immunohistochemistry (PFA fixed) - Anti-Eagle antibody [8B11DH10] (AB201327)
  • IHC (PFA fixed)

Supplier Data

Immunohistochemistry (PFA fixed) - Anti-Eagle antibody [8B11DH10] (AB201327)

Immunohistochemistry analysis in neuroblast nuclei from Drosophila stage 15 embryos. The cells were fixed in 4% formaldehyde for 25 minutes, permeabilized with 0.1% Triton X-100/PBS, and blocked in 0.1% Triton X-100/PBS, 2.5% Normal Goat Serum, 2.5% Normal Donkey Serum.The Eagle antibody (ab201327) was used at 3.8 μg/mL, overnight at 4°C. The secondary antibody used was Alexa488 conjugated goat anti-mouse at 1/400.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8B11DH10

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Drosophila melanogaster

Applications

IHC (PFA fixed)

applications

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCPFAfixed" : {"fullname" : "Immunohistochemistry (PFA fixed)", "shortname":"IHC (PFA fixed)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Drosophila melanogaster": { "IHCPFAfixed-species-checked": "testedAndGuaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "<p></p>" } } }
style
left-column

Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
style
left-column

Properties and storage information

Form
Liquid
Purification notes
Purity: 95%
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: 99% HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Eagle also known as ELK3 or Net is a nuclear protein that weighs approximately 44 kDa. It is a member of the ETS domain transcription factor family. Eagle primarily localizes in the nucleus and is widely expressed in various cell types with higher concentrations observed in the spleen and thymus. The protein shows a significant affinity for binding to DNA sequences and influences the transcriptional regulation of genes involved in cellular proliferation and differentiation.
Biological function summary

Eagle plays a significant role in the regulation of gene expression. It functions as part of a multiprotein complex involving other ETS domain family members. Eagle can act as both a transcriptional activator and repressor depending on the cellular context. Its regulatory activities are important in the processes of vascular development and maintenance of normal cellular function.

Pathways

Eagle is actively involved in the Ras-MAPK signaling pathway. This pathway is important for transmitting signals from the cell surface to the nucleus controlling cell cycle progression and modulating differentiation responses. Eagle interacts closely with other proteins in this pathway such as MEK and ERK influencing the transcriptional regulation downstream of the signaling cascade. Eagle's participation in this pathway reflects its ability to affect various cellular outcomes through modulating gene expression.

Eagle has been associated with several types of cancer including breast and colorectal cancer. Its dysregulation affects the transcriptional control of genes linked to uncontrolled cell proliferation. Eagle's interaction with the protein c-Fos in these cancers contributes to altered signaling pathways that favor oncogenesis. Understanding Eagle's role in these diseases highlights its potential as a target for therapeutic intervention in cancer research.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

May play a critical role in development of the progeny of eagle-positive neuroblasts.
See full target information eg
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS biology 23:e3002881 PubMed40163536

2025

The Hunchback transcription factor determines interneuron molecular identity, morphology, and presynapse targeting in the Drosophila NB5-2 lineage.

Applications

Unspecified application

Species

Unspecified reactive species

Heather Q Pollington,Chris Q Doe
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB124927

Anti-ZNF217 antibody [EPR6362]

RabMAb|Recombinant

Western blot - Anti-ZNF217 antibody [EPR6362] (AB124927)

  • 3
  • 1
Primary Antibodies

AB157459

Anti-ZNF365 antibody [EPR10216]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-ZNF365 antibody [EPR10216] (AB157459)

  • 0
  • 0
Primary Antibodies

AB157220

Anti-VPS35 antibody [EPR11501(B)]

KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-VPS35 antibody [EPR11501(B)] (AB157220)

  • 2
  • 1
Primary Antibodies

AB109219

Anti-USP10 antibody [EPR4261]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP10 antibody [EPR4261] (AB109219)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com