Rabbit Polyclonal EAP30 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SNF8 aa 50-200.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SNF8 aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q96H20
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/50.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Zebrafish | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Zebrafish | Dilution info - | Notes - |
Target data
Function
Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs, and plays a role in autophagy (PubMed:38423010). The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).
Alternative names
EAP30, SNF8, Vacuolar-sorting protein SNF8, ELL-associated protein of 30 kDa, ESCRT-II complex subunit VPS22, hVps22
Recommended products
Rabbit Polyclonal EAP30 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SNF8 aa 50-200.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SNF8 aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q96H20
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EAP30 also known as VPS22 or SNF8 is a component of the ESCRT-II complex with a mass of approximately 30 kDa. It plays an important role in the endosomal sorting process a mechanism that directs the transport of ubiquitinated transmembrane proteins for lysosomal degradation. EAP30 is expressed in various tissues but shows high expression levels in cells involved in membrane trafficking and processes requiring active protein sorting.
- Biological function summary
The EAP30 protein functions as part of the ESCRT-II complex which is essential for the formation of multivesicular bodies (MVBs) and their sorting machinery. EAP30 interacts with other components of the ESCRT machinery such as EAP20 and VPS36 ensuring effective processing of cellular signaling receptors and achieving membrane dynamics. This interaction facilitates the correct trafficking of membrane proteins which is necessary for maintaining cellular homeostasis.
- Pathways
EAP30 is deeply integrated into the endosomal-lysosomal pathway which is essential for directing proteins toward lysosomal degradation. The protein operates closely with the ESCRT complex including ESCRT-I and ESCRT-III and is involved in endocytosis and receptor downregulation. It coordinates with proteins like VPS4 during late stages of vesicle budding and abscission essential for accurate vesicle scission events.
- Associated diseases and disorders
Malfunctions in EAP30 activities can relate to neurodegenerative conditions such as Alzheimer's disease and frontotemporal dementia. EAP30's incorrect function could impair protein degradation processes leading to the accumulation of ubiquitinated proteins. The protein also shows connections with components like TSG101 which further roles in vesicular transport perturbations seen in these diseases indicating possible therapeutic targets for intervention strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-EAP30 antibody (ab251956)
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for EAP30 (green) using ab251956 at 4 µg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAP30 antibody (ab251956)
Paraffin-embedded human gallbladder tissue stained for EAP30 using ab251956 at 1/20 dilution in immunohistochemical analysis.
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