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AB169860

Anti-EAPP antibody [1E4]

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(1 Publication)

Mouse Monoclonal EAPP antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EAPP.

View Alternative Names

C14orf11, BM-036, EAPP, E2F-associated phosphoprotein

1 Images
Western blot - Anti-EAPP antibody [1E4] (AB169860)
  • WB

Supplier Data

Western blot - Anti-EAPP antibody [1E4] (AB169860)

Lane 1:

Western blot - Anti-EAPP antibody [1E4] (ab169860) at 1/50 dilution

Lane 2:

Western blot - Anti-EAPP antibody [1E4] (ab169860) at 1/200 dilution

Lane 3:

Western blot - Anti-EAPP antibody [1E4] (ab169860) at 1/1000 dilution

Lane 4:

Western blot - Anti-EAPP antibody [1E4] (ab169860) at 1/2000 dilution

All lanes:

HeLa cell lysate

Secondary

All lanes:

Anti-mouse IgG (whole molecule)-AP conjugate at 1/2000 dilution

Predicted band size: 33 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1E4

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human EAPP. The exact immunogen used to generate this antibody is proprietary information.

Q56P03

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50 - 1/200", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
ab166860 was purified from supernatant.
Storage buffer
Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EAPP also known as E2F-associated phosphoprotein is an important regulator in the cell cycle. It weighs approximately 37 kDa and is seen expressed mainly in the nucleus of proliferating cells. As a phosphoprotein it binds to E2F transcription factors influencing their activity. EAPP modulates processes associated with cell division and response to DNA damage reflecting its involvement in growth-related cellular mechanisms.
Biological function summary

EAPP acts as a modulator for E2F transcription factors which play central roles in controlling the expression of genes necessary for DNA replication and mitosis. By associating with E2F proteins EAPP contributes to the regulation of the cell cycle particularly promoting the transition from G1 to S phase. Although EAPP is not part of a stable complex by itself its interaction with other cell cycle proteins makes it significant in cell proliferation and checkpoint control.

Pathways

EAPP participates in the regulation of the cell cycle and DNA repair pathways. It interacts closely with E2F1 an important player in G1/S transition and apoptosis. Sensing DNA damage EAPP fine-tunes the cell's repair response working alongside proteins like pRB and Cyclin A. Its interactions suggest a role in maintaining genomic stability by facilitating proper cell cycle progression and ensuring effective repair mechanisms.

EAPP has connections to cancer and genomic instability disorders. Overexpression or misregulation of EAPP can lead to uncontrolled cell proliferation contributing to oncogenesis. Its interaction with E2F1 is important as disruptions in this relationship can enhance tumor progression. Additionally EAPP's role in DNA repair connects it to disorders characterized by defective DNA damage response highlighting its potential as a therapeutic target in precision medicine.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play an important role in the fine-tuning of both major E2F1 activities, the regulation of the cell-cycle and the induction of apoptosis. Promotes S-phase entry, and inhibits p14(ARP) expression.
See full target information EAPP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 8:17835 PubMed30546028

2018

Experimental Metabolic Syndrome Model Associated with Mechanical and Structural Degenerative Changes of the Aortic Valve.

Applications

Unspecified application

Species

Unspecified reactive species

Jason L Go,Komal Prem,Mohammed A Al-Hijji,Qing Qin,Christopher Noble,Melissa D Young,Lilach O Lerman,Amir Lerman
View all publications
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Product promise

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