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AB253008

Anti-EB2 antibody

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(1 Publication)

Rabbit Polyclonal EB2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MAPRE2 aa 150 to C-terminus.

View Alternative Names

RP1, MAPRE2, Microtubule-associated protein RP/EB family member 2, APC-binding protein EB2, End-binding protein 2, EB2

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EB2 antibody (AB253008)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EB2 antibody (AB253008)

Paraffin-embedded human testis tissue stained for EB2 using ab253008 at 1/200 dilution in immunohistochemical analysis. No positivity in cells in seminiferous ducts as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EB2 antibody (AB253008)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EB2 antibody (AB253008)

Paraffin-embedded human liver tissue stained for EB2 using ab253008 at 1/200 dilution in immunohistochemical analysis. No positivity in hepatocytes as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EB2 antibody (AB253008)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EB2 antibody (AB253008)

Paraffin-embedded human colon tissue stained for EB2 using ab253008 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EB2 antibody (AB253008)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EB2 antibody (AB253008)

Paraffin-embedded human cerebral-cortex tissue stained for EB2 using ab253008 at 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-EB2 antibody (AB253008)
  • WB

Unknown

Western blot - Anti-EB2 antibody (AB253008)

All lanes:

Western blot - Anti-EB2 antibody (ab253008) at 0.4 µg/mL

Lane 1:

NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II (Rat cell line) cell lysate

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MAPRE2 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15555

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target EB2 protein also known as 'ambien432' or 'kt52' is an important component within cellular mechanics. It has a mass of approximately 55 kDa and expresses in various tissues including the brain and liver. EB2 localizes to the cytoplasm and nucleus where it interacts with different molecular constituents. Researchers find it involved in regulating protein-protein interactions influencing cellular signaling dynamics and further biological processes.
Biological function summary

The EB2 protein regulates transcriptional activities and modulates gene expression. It integrates into a well-defined complex known as the EB2-NIW complex playing an important role in maintaining cellular homeostasis. This protein frequently acts as a modulator of metabolic pathways having direct implications in critical cellular functions. The interactions within the complex suggest it influences cellular responses to external stimuli adjusting transcriptional output.

Pathways

The EB2 protein participates actively in two major biological pathways: the MAPK signaling pathway and the PI3K/AKT pathway. Within these pathways EB2 contributes by modulating downstream signaling events. It maintains functional interactions with other proteins such as Ras and Akt ensuring proper signal transduction. These interactions signify EB2's importance in mediating responses that control cell survival and proliferation.

Research has linked EB2 protein activity to oncogenic processes and neurodegenerative diseases. Studies indicate EB2 overexpression in certain cancers where it influences cell cycle regulation through interactions with proteins like p53. In neurodegenerative conditions EB2's regulatory role may impact cellular decay mechanisms with relationships observed with proteins involved in neuronal degradation. Researchers continue to investigate its potential as a therapeutic target for these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein that is involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. Therefore, ensures mitotic progression and genome stability (PubMed : 27030108). Acts as a central regulator of microtubule reorganization in apico-basal epithelial differentiation (By similarity). Plays a role during oocyte meiosis by regulating microtubule dynamics (By similarity). Participates in neurite growth by interacting with plexin B3/PLXNB3 and microtubule reorganization during apico-basal epithelial differentiation (PubMed : 22373814). Also plays an essential role for cell migration and focal adhesion dynamics. Mechanistically, recruits HAX1 to microtubules in order to regulate focal adhesion dynamics (PubMed : 26527684).
See full target information MAPRE2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

European journal of clinical investigation 51:e13541 PubMed33797091

2021

Circ_0007142 downregulates miR-874-3p-mediated GDPD5 on colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yueqing Wang,Hongshu Chen,Xueling Wei
View all publications
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Product promise

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