AB45767

Anti-EB2 antibody [KT52]

Name: Anti-EB2 antibody [KT52] (ab45767) | Abcam
Brand: Abcam Limited
SKU: AB45767
Price: 410 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(11 Publications)

Rat Monoclonal EB2 antibody. Suitable for ICC/IF and reacts with Recombinant full length protein - Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MAPRE2.

View Alternative Names

RP1, MAPRE2, Microtubule-associated protein RP/EB family member 2, APC-binding protein EB2, End-binding protein 2, EB2

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-EB2 antibody [KT52] (AB45767)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EB2 antibody [KT52] (AB45767)

Immunofluorescence staining of EB2 in CHO cells transfected with EB2 gene expression constructs.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

KT52

Isotype

IgG2b

Carrier free

Applications

ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MAPRE2.

Q15555

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein - Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target EB2 protein also known as 'ambien432' or 'kt52' is an important component within cellular mechanics. It has a mass of approximately 55 kDa and expresses in various tissues including the brain and liver. EB2 localizes to the cytoplasm and nucleus where it interacts with different molecular constituents. Researchers find it involved in regulating protein-protein interactions influencing cellular signaling dynamics and further biological processes.

Biological function summary

The EB2 protein regulates transcriptional activities and modulates gene expression. It integrates into a well-defined complex known as the EB2-NIW complex playing an important role in maintaining cellular homeostasis. This protein frequently acts as a modulator of metabolic pathways having direct implications in critical cellular functions. The interactions within the complex suggest it influences cellular responses to external stimuli adjusting transcriptional output.

Pathways

The EB2 protein participates actively in two major biological pathways: the MAPK signaling pathway and the PI3K/AKT pathway. Within these pathways EB2 contributes by modulating downstream signaling events. It maintains functional interactions with other proteins such as Ras and Akt ensuring proper signal transduction. These interactions signify EB2's importance in mediating responses that control cell survival and proliferation.

Research has linked EB2 protein activity to oncogenic processes and neurodegenerative diseases. Studies indicate EB2 overexpression in certain cancers where it influences cell cycle regulation through interactions with proteins like p53. In neurodegenerative conditions EB2's regulatory role may impact cellular decay mechanisms with relationships observed with proteins involved in neuronal degradation. Researchers continue to investigate its potential as a therapeutic target for these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter protein that is involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. Therefore, ensures mitotic progression and genome stability (PubMed : 27030108). Acts as a central regulator of microtubule reorganization in apico-basal epithelial differentiation (By similarity). Plays a role during oocyte meiosis by regulating microtubule dynamics (By similarity). Participates in neurite growth by interacting with plexin B3/PLXNB3 and microtubule reorganization during apico-basal epithelial differentiation (PubMed : 22373814). Also plays an essential role for cell migration and focal adhesion dynamics. Mechanistically, recruits HAX1 to microtubules in order to regulate focal adhesion dynamics (PubMed : 26527684).

See full target information MAPRE2

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Neuroscience bulletin 41:805-820 PubMed40042769

2025

A Novel Mouse Model Unveils Protein Deficiency in Truncated CDKL5 Mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Feng,Zi-Ai Zhu,Hong-Tao Wang,Hui-Wen Zhou,Ji-Wei Liu,Ya Shen,Yu-Xian Zhang,Zhi-Qi Xiong

Cell reports. Medicine 6:101926 PubMed39855191

2025

Independent genetic strategies define the scope and limits of CDKL5 deficiency disorder reversal.

Applications

Unspecified application

Species

Unspecified reactive species

Xie Song,Zijie Xia,Dayne Martinez,Bing Xu,Zachary Spritzer,Yanjie Zhang,Erin Nugent,Yugong Ho,Barbara Terzic,Zhaolan Zhou

Molecular psychiatry 29:1844-1856 PubMed38326557

2024

Cell type-specific expression, regulation and compensation of CDKL5 activity in mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Margaux Silvestre,Kelvin Dempster,Simeon R Mihaylov,Suzanne Claxton,Sila K Ultanir

eLife 12: PubMed37490324

2023

Discovery and characterization of a specific inhibitor of serine-threonine kinase cyclin-dependent kinase-like 5 (CDKL5) demonstrates role in hippocampal CA1 physiology.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Castano,Margaux Silvestre,Carrow I Wells,Jennifer L Sanderson,Carla A Ferrer,Han Wee Ong,Yi Lang,William Richardson,Josie A Silvaroli,Frances M Bashore,Jeffery L Smith,Isabelle M Genereux,Kelvin Dempster,David H Drewry,Navlot S Pabla,Alex N Bullock,Tim A Benke,Sila K Ultanir,Alison D Axtman

ACS chemical neuroscience 14:1672-1685 PubMed37084253

2023

Discovery of a Potent and Selective CDKL5/GSK3 Chemical Probe That Is Neuroprotective.

Applications

Unspecified application

Species

Unspecified reactive species

Han Wee Ong,Yi Liang,William Richardson,Emily R Lowry,Carrow I Wells,Xiangrong Chen,Margaux Silvestre,Kelvin Dempster,Josie A Silvaroli,Jeffery L Smith,Hynek Wichterle,Navjot S Pabla,Sila K Ultanir,Alex N Bullock,David H Drewry,Alison D Axtman

The Journal of clinical investigation 131: PubMed34651584

2021

Temporal manipulation of Cdkl5 reveals essential postdevelopmental functions and reversible CDKL5 deficiency disorder-related deficits.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Terzic,M Felicia Davatolhagh,Yugong Ho,Sheng Tang,Yu-Ting Liu,Zijie Xia,Yue Cui,Marc V Fuccillo,Zhaolan Zhou

Neurobiology of disease 155:105370 PubMed33905871

2021

Abnormalities of mitochondrial dynamics and bioenergetics in neuronal cells from CDKL5 deficiency disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole J Van Bergen,Sean Massey,Tegan Stait,Molly Ellery,Boris Reljić,Luke E Formosa,Anita Quigley,Mirella Dottori,David Thorburn,David A Stroud,John Christodoulou

Frontiers in oncology 10:461 PubMed32426268

2020

WDHD1 Leads to Cisplatin Resistance by Promoting MAPRE2 Ubiquitination in Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lian Gong,Mengqing Xiao,Dong He,Yi Hu,Yuxing Zhu,Liang Xiang,Ying Bao,Xiaoming Liu,Qinghai Zeng,Jianye Liu,Ming Zhou,Yanhong Zhou,Yaxin Cheng,Yeyu Zhang,Liping Deng,Rongrong Zhu,Hua Lan,Ke Cao

Nature communications 10:2655 PubMed31201320

2019

Altered NMDAR signaling underlies autistic-like features in mouse models of CDKL5 deficiency disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Tang,Barbara Terzic,I-Ting Judy Wang,Nicolas Sarmiento,Katherine Sizov,Yue Cui,Hajime Takano,Eric D Marsh,Zhaolan Zhou,Douglas A Coulter

The EMBO journal 37: PubMed30266824

2018

Chemical genetic identification of CDKL5 substrates reveals its role in neuronal microtubule dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Lucas L Baltussen,Priscilla D Negraes,Margaux Silvestre,Suzanne Claxton,Max Moeskops,Evangelos Christodoulou,Helen R Flynn,Ambrosius P Snijders,Alysson R Muotri,Sila K Ultanir

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EB2 antibody [KT52]

Immunocytochemistry/ Immunofluorescence - Anti-EB2 antibody [KT52] (AB45767)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

Alternative Products

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Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise