Anti-EB3 antibody [KT36]

• Flow Cyt

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Flow Cytometry - Anti-EB3 antibody [KT36] (AB53360)

Overlay histogram showing SH-SY5Y cells stained with ab53360 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53360, 2μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-EB3 antibody [KT36] (AB53360)

Immunofluorescent staining of cell mitosis using ab53360 crude culture supernatant diluted 1 : 10 (Please note that there was about 50-100 ug/ml antibody in the supernatants) COS7 Transformed African Green Monkey Kidney Fibroblast Cells.

This image is courtesy of Dr Anna Akhmanova

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EB3 antibody [KT36] (AB53360)

Immunofluorescent staining for COS7 cells overexpressing EB3, using ab53360 at 1/100.

This image is courtesy of Dr Anna Akhmanova

• WB

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Western blot - Anti-EB3 antibody [KT36] (AB53360)

All lanes:

Western blot - Anti-EB3 antibody [KT36] (ab53360) at 1 µg/mL

All lanes:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Secondary

All lanes:

Peroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilution

Predicted band size: 31 kDa

Observed band size: 32 kDa

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Exposure time: 8min