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AB126135

Anti-EBF1 antibody

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(4 Publications)

Rabbit Polyclonal EBF1 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EBF1 aa 50-350.

View Alternative Names

COE1, EBF, EBF1, Transcription factor COE1, O/E-1, OE-1, Early B-cell factor

1 Images
Western blot - Anti-EBF1 antibody (AB126135)
  • WB

Unknown

Western blot - Anti-EBF1 antibody (AB126135)

All lanes:

Western blot - Anti-EBF1 antibody (ab126135) at 1/1000 dilution

All lanes:

THP-1 whole cell lysate at 30 µg

Predicted band size: 64 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human EBF1 aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

Q9UH73

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EBF1 also known as Early B-cell Factor 1 is a transcription factor with a molecular mass of approximately 65 kDa. This protein plays a critical role in the development and differentiation of B-cells. EBF1 is expressed mainly in developing B-cells as well as some neurons indicating its role in the immune and nervous systems. The protein contains a helix-loop-helix motif which helps it bind to DNA and modulate gene expression.
Biological function summary

Early B-cell Factor 1 serves as a major regulator during B-cell development. It contributes to the transcriptional network essential for the maturation of progenitor cells into fully functional B-cells. Although not typically found in a larger complex EBF1 interacts with other transcription factors to ensure proper lineage differentiation and function. Its activity influences the expression of various genes important for immune response.

Pathways

Early B-cell Factor 1 connects deeply with the B-cell receptor signaling pathway which is essential for B-cell survival and proliferation. EBF1 often interacts through this pathway with proteins like PAX5 which also plays an important role in B-cell lineage commitment. Additionally EBF1 can interact with the Notch signaling pathway which affects cellular differentiation processes during development.

Dysregulation of Early B-cell Factor 1 activity often associates with immunological diseases such as acute lymphoblastic leukemia (ALL) and B-cell lymphomas. Aberrant expression levels or mutations in EBF1 can disrupt normal B-cell functioning and lead to such malignancies. EBF1 is linked intricately with proteins like TCF3 where aberrations in both can lead to leukemic conditions emphasizing its role in maintaining cellular signaling balance in immune-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key pioneer transcription factor of B-cell specification and commitment (PubMed : 27807034). Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Operates in a transcription factor network to activate B-cell-specific genes and repress genes associated with alternative cell fates. For instance, positively regulates many B-cell specific genes including BCR or CD40 while repressing genes that direct cells into alternative lineages, including GATA3 and TCF7 for the T-cell lineage. In addition to its role during lymphopoiesis, controls the thermogenic gene program in adipocytes during development and in response to environmental cold (By similarity).. (Microbial infection) Acts as a chromatin anchor for Epstein-Barr virus EBNA2 to mediate the assembly of EBNA2 chromatin complexes in B-cells (PubMed : 28968461). In addition, binds to the viral LMP1 proximal promoter and promotes its expression during latency (PubMed : 26819314).
See full target information EBF1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Histochemistry and cell biology 160:51-61 PubMed37115319

2023

MicroRNA-598 inhibition ameliorates LPS-induced acute lung injury in mice through upregulating Ebf1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Zhao,Lei He,Junwu Zhang,Hong Li,Wanying Li,Zhihui Zhou,Yuanyuan Li

Scientific reports 11:1537 PubMed33452395

2021

EBF1 drives hallmark B cell gene expression by enabling the interaction of PAX5 with the MLL H3K4 methyltransferase complex.

Applications

Unspecified application

Species

Unspecified reactive species

Charles E Bullerwell,Philippe Pierre Robichaud,Pierre M L Deprez,Andrew P Joy,Gabriel Wajnberg,Darwin D'Souza,Simi Chacko,Sébastien Fournier,Nicolas Crapoulet,David A Barnett,Stephen M Lewis,Rodney J Ouellette

The Journal of comparative neurology 526:397-411 PubMed28921616

2017

Tangential migration of corridor guidepost neurons contributes to anxiety circuits.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Tinterri,Marie Deck,Maryama Keita,Caroline Mailhes,Anna Noren Rubin,Nicoletta Kessaris,Ludmilla Lokmane,Franck Bielle,Sonia Garel

International journal of molecular medicine 33:1355-63 PubMed24626634

2014

Identification of hypo- and hypermethylated genes related to atherosclerosis by a genome-wide analysis of DNA methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshiji Yamada,Tamotsu Nishida,Hideki Horibe,Mitsutoshi Oguri,Kimihiko Kato,Motoji Sawabe
View all publications
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