Rabbit Polyclonal EBF1 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EBF1 aa 50-350.
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Publications
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- Scientific reports 11:15372021EBF1 drives hallmark B cell gene expression by enabling the interaction of PAX5 with the MLL H3K4 methyltransferase complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharles E Bullerwell,Philippe Pierre Robichaud,Pierre M L Deprez,Andrew P Joy,Gabriel Wajnberg,Darwin D'Souza,Simi Chacko,Sébastien Fournier,Nicolas Crapoulet,David A Barnett,Stephen M Lewis,Rodney J OuellettePubMed 33452395
- The Journal of comparative neurology 526:397-4112017Tangential migration of corridor guidepost neurons contributes to anxiety circuits.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrea Tinterri,Marie Deck,Maryama Keita,Caroline Mailhes,Anna Noren Rubin,Nicoletta Kessaris,Ludmilla Lokmane,Franck Bielle,Sonia GarelPubMed 28921616
- International journal of molecular medicine 33:1355-632014Identification of hypo- and hypermethylated genes related to atherosclerosis by a genome-wide analysis of DNA methylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoshiji Yamada et. al.PubMed 24626634
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human EBF1 aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UH73
Consider this alternative
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Chicken | Predicted |
| Cow | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow | Dilution info - | Notes - |
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Target data
Function
Key pioneer transcription factor of B-cell specification and commitment (PubMed:27807034). Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Operates in a transcription factor network to activate B-cell-specific genes and repress genes associated with alternative cell fates. For instance, positively regulates many B-cell specific genes including BCR or CD40 while repressing genes that direct cells into alternative lineages, including GATA3 and TCF7 for the T-cell lineage. In addition to its role during lymphopoiesis, controls the thermogenic gene program in adipocytes during development and in response to environmental cold (By similarity).(Microbial infection) Acts as a chromatin anchor for Epstein-Barr virus EBNA2 to mediate the assembly of EBNA2 chromatin complexes in B-cells (PubMed:28968461). In addition, binds to the viral LMP1 proximal promoter and promotes its expression during latency (PubMed:26819314).
Alternative names
Transcription factor COE1, O/E-1, OE-1, Early B-cell factor, EBF, COE1, EBF1
Recommended products
Rabbit Polyclonal EBF1 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EBF1 aa 50-350.
Alternative names
Transcription factor COE1, O/E-1, OE-1, Early B-cell factor, EBF, COE1, EBF1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human EBF1 aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UH73
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EBF1 also known as Early B-cell Factor 1 is a transcription factor with a molecular mass of approximately 65 kDa. This protein plays a critical role in the development and differentiation of B-cells. EBF1 is expressed mainly in developing B-cells as well as some neurons indicating its role in the immune and nervous systems. The protein contains a helix-loop-helix motif which helps it bind to DNA and modulate gene expression.
- Biological function summary
Early B-cell Factor 1 serves as a major regulator during B-cell development. It contributes to the transcriptional network essential for the maturation of progenitor cells into fully functional B-cells. Although not typically found in a larger complex EBF1 interacts with other transcription factors to ensure proper lineage differentiation and function. Its activity influences the expression of various genes important for immune response.
- Pathways
Early B-cell Factor 1 connects deeply with the B-cell receptor signaling pathway which is essential for B-cell survival and proliferation. EBF1 often interacts through this pathway with proteins like PAX5 which also plays an important role in B-cell lineage commitment. Additionally EBF1 can interact with the Notch signaling pathway which affects cellular differentiation processes during development.
- Associated diseases and disorders
Dysregulation of Early B-cell Factor 1 activity often associates with immunological diseases such as acute lymphoblastic leukemia (ALL) and B-cell lymphomas. Aberrant expression levels or mutations in EBF1 can disrupt normal B-cell functioning and lead to such malignancies. EBF1 is linked intricately with proteins like TCF3 where aberrations in both can lead to leukemic conditions emphasizing its role in maintaining cellular signaling balance in immune-related disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-EBF1 antibody (ab126135)
All lanes: Western blot - Anti-EBF1 antibody (ab126135) at 1/1000 dilution
All lanes: THP-1 whole cell lysate at 30 µg
Performed under reducing conditions.
Predicted band size: 64 kDa
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