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AB221033

Anti-EBF1 antibody

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(2 Publications)

Rabbit Polyclonal EBF1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EBF1 aa 1-50.

View Alternative Names

COE1, EBF, EBF1, Transcription factor COE1, O/E-1, OE-1, Early B-cell factor

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-EBF1 antibody (AB221033)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EBF1 antibody (AB221033)

Immunofluorescent analysis of PFA fixed, triton X-100 RH-30 cells labeling EBF1 with ab221033 at 4 μg/mL (green).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human EBF1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9UH73

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EBF1 also known as Early B-cell Factor 1 is a transcription factor with a molecular mass of approximately 65 kDa. This protein plays a critical role in the development and differentiation of B-cells. EBF1 is expressed mainly in developing B-cells as well as some neurons indicating its role in the immune and nervous systems. The protein contains a helix-loop-helix motif which helps it bind to DNA and modulate gene expression.
Biological function summary

Early B-cell Factor 1 serves as a major regulator during B-cell development. It contributes to the transcriptional network essential for the maturation of progenitor cells into fully functional B-cells. Although not typically found in a larger complex EBF1 interacts with other transcription factors to ensure proper lineage differentiation and function. Its activity influences the expression of various genes important for immune response.

Pathways

Early B-cell Factor 1 connects deeply with the B-cell receptor signaling pathway which is essential for B-cell survival and proliferation. EBF1 often interacts through this pathway with proteins like PAX5 which also plays an important role in B-cell lineage commitment. Additionally EBF1 can interact with the Notch signaling pathway which affects cellular differentiation processes during development.

Dysregulation of Early B-cell Factor 1 activity often associates with immunological diseases such as acute lymphoblastic leukemia (ALL) and B-cell lymphomas. Aberrant expression levels or mutations in EBF1 can disrupt normal B-cell functioning and lead to such malignancies. EBF1 is linked intricately with proteins like TCF3 where aberrations in both can lead to leukemic conditions emphasizing its role in maintaining cellular signaling balance in immune-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key pioneer transcription factor of B-cell specification and commitment (PubMed : 27807034). Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Operates in a transcription factor network to activate B-cell-specific genes and repress genes associated with alternative cell fates. For instance, positively regulates many B-cell specific genes including BCR or CD40 while repressing genes that direct cells into alternative lineages, including GATA3 and TCF7 for the T-cell lineage. In addition to its role during lymphopoiesis, controls the thermogenic gene program in adipocytes during development and in response to environmental cold (By similarity).. (Microbial infection) Acts as a chromatin anchor for Epstein-Barr virus EBNA2 to mediate the assembly of EBNA2 chromatin complexes in B-cells (PubMed : 28968461). In addition, binds to the viral LMP1 proximal promoter and promotes its expression during latency (PubMed : 26819314).
See full target information EBF1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cells 11: PubMed36497195

2022

Caveolin-1 rs1997623 Single Nucleotide Polymorphism Creates a New Binding Site for the Early B-Cell Factor 1 That Instigates Adipose Tissue CAV1 Protein Overexpression.

Applications

Unspecified application

Species

Unspecified reactive species

Ashraf Al Madhoun,Dania Haddad,Rasheeba Nizam,Lavina Miranda,Shihab Kochumon,Reeby Thomas,Thangavel Alphonse Thanaraj,Rasheed Ahmad,Milad S Bitar,Fahd Al-Mulla

Oncology reports 44:139-155 PubMed32377743

2020

miR‑193b exhibits mutual interaction with MYC, and suppresses growth and metastasis of osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jinjian Gao,Sai Ma,Fan Yang,Xu Chen,Wei Wang,Jianping Zhang,Yufang Li,Tao Wang,Lequn Shan
View all publications
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Product promise

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