AB108369

Anti-EBF1 antibody [EPR4183]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(10 Publications)

Rabbit Recombinant Monoclonal EBF1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 10 publications.

View Alternative Names

COE1, EBF, EBF1, Transcription factor COE1, O/E-1, OE-1, Early B-cell factor

4 Images

Supplier Data

Western blot - Anti-EBF1 antibody [EPR4183] (AB108369)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-EBF1 antibody [EPR4183] (ab108369) at 1/1000 dilution

All lanes:

Raji cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 20 µg

Predicted band size: 64 kDa

false

Unknown

Western blot - Anti-EBF1 antibody [EPR4183] (AB108369)

We are unsure how to define the extra bands.

All lanes:

Western blot - Anti-EBF1 antibody [EPR4183] (ab108369) at 1/1000 dilution

Lane 1:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 2:

Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

Lane 3:

Mouse spleen lysate at 20 µg

Lane 4:

Mouse lymph node lysate at 20 µg

Lane 5:

Rat spleen lysate at 20 µg

Lane 6:

Rat lymph node lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 64 kDa

Observed band size: 64 kDa

false

Lab

Western blot - Anti-EBF1 antibody [EPR4183] (AB108369)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This protein is easy to be degraded, to minimize protein degradation, cells/tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting right away.

All lanes:

Western blot - Anti-EBF1 antibody [EPR4183] (ab108369) at 1/1000 dilution

Lane 1:

Mouse spleen frozen lysate at 20 µg

Lane 2:

Mouse spleen fresh lysate at 20 µg

Lane 3:

Rat spleen frozen lysate at 20 µg

Lane 4:

Rat spleen fresh lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 70 kDa

false

Exposure time: 180s

Unknown

Western blot - Anti-EBF1 antibody [EPR4183] (AB108369)

All lanes:

Western blot - Anti-EBF1 antibody [EPR4183] (ab108369) at 1/1000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

Daudi cell lysate at 10 µg

Lane 3:

3T3-LI cell lysate at 10 µg

Predicted band size: 64 kDa

false

Carrier free

Anti-EBF1 antibody [EPR4183] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4183

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody also recognises EBF3 protein.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "For unpurified use at 1/10000.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "For unpurified use at 1/10000.This protein is easy to be degraded, to minimize protein degradation, cells/tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting right away.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "For unpurified use at 1/10000.This protein is easy to be degraded, to minimize protein degradation, cells/tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting right away.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EBF1 also known as Early B-cell Factor 1 is a transcription factor with a molecular mass of approximately 65 kDa. This protein plays a critical role in the development and differentiation of B-cells. EBF1 is expressed mainly in developing B-cells as well as some neurons indicating its role in the immune and nervous systems. The protein contains a helix-loop-helix motif which helps it bind to DNA and modulate gene expression.

Biological function summary

Early B-cell Factor 1 serves as a major regulator during B-cell development. It contributes to the transcriptional network essential for the maturation of progenitor cells into fully functional B-cells. Although not typically found in a larger complex EBF1 interacts with other transcription factors to ensure proper lineage differentiation and function. Its activity influences the expression of various genes important for immune response.

Pathways

Early B-cell Factor 1 connects deeply with the B-cell receptor signaling pathway which is essential for B-cell survival and proliferation. EBF1 often interacts through this pathway with proteins like PAX5 which also plays an important role in B-cell lineage commitment. Additionally EBF1 can interact with the Notch signaling pathway which affects cellular differentiation processes during development.

Dysregulation of Early B-cell Factor 1 activity often associates with immunological diseases such as acute lymphoblastic leukemia (ALL) and B-cell lymphomas. Aberrant expression levels or mutations in EBF1 can disrupt normal B-cell functioning and lead to such malignancies. EBF1 is linked intricately with proteins like TCF3 where aberrations in both can lead to leukemic conditions emphasizing its role in maintaining cellular signaling balance in immune-related disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Key pioneer transcription factor of B-cell specification and commitment (PubMed : 27807034). Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Operates in a transcription factor network to activate B-cell-specific genes and repress genes associated with alternative cell fates. For instance, positively regulates many B-cell specific genes including BCR or CD40 while repressing genes that direct cells into alternative lineages, including GATA3 and TCF7 for the T-cell lineage. In addition to its role during lymphopoiesis, controls the thermogenic gene program in adipocytes during development and in response to environmental cold (By similarity).. (Microbial infection) Acts as a chromatin anchor for Epstein-Barr virus EBNA2 to mediate the assembly of EBNA2 chromatin complexes in B-cells (PubMed : 28968461). In addition, binds to the viral LMP1 proximal promoter and promotes its expression during latency (PubMed : 26819314).

See full target information EBF1

style

left-column

Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:390 PubMed38555395

2024

CircEYA3 aggravates intervertebral disc degeneration through the miR-196a-5p/EBF1 axis and NF-κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Tianfu Wang,Xiaobing Yan,Dehui Song,Yingxia Li,Zhengwei Li,Dapeng Feng

Cells 11: PubMed36497195

2022

Caveolin-1 rs1997623 Single Nucleotide Polymorphism Creates a New Binding Site for the Early B-Cell Factor 1 That Instigates Adipose Tissue CAV1 Protein Overexpression.

Applications

Unspecified application

Species

Unspecified reactive species

Ashraf Al Madhoun,Dania Haddad,Rasheeba Nizam,Lavina Miranda,Shihab Kochumon,Reeby Thomas,Thangavel Alphonse Thanaraj,Rasheed Ahmad,Milad S Bitar,Fahd Al-Mulla

Clinical and translational medicine 11:e479 PubMed34323408

2021

Long noncoding RNA IL6-AS1 is highly expressed in chronic obstructive pulmonary disease and is associated with interleukin 6 by targeting miR-149-5p and early B-cell factor 1.

Applications

Unspecified application

Species

Unspecified reactive species

Erkang Yi,Jiahuan Zhang,Mengning Zheng,Yi Zhang,Chunxiao Liang,Binwei Hao,Wei Hong,Biting Lin,Jinding Pu,Zhiwei Lin,Peiyu Huang,Bing Li,Yumin Zhou,Pixin Ran

Thyroid : official journal of the American Thyroid : PubMed33267733

2021

Thyroid Hormone Receptor α1 Mutants Impair B Lymphocyte Development in a Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Sunmi Park,Xuguang Zhu,Minjun Kim,Li Zhao,Sheue-Yann Cheng

Journal of cellular biochemistry 120:1407-1419 PubMed30335886

2018

Retracted: EBF1 gene promotes the proliferation and inhibits the apoptosis of bone marrow CD34+ cells in patients with myelodysplastic syndrome through negative regulation of mitogen-activated protein kinase axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Hou,Jie Hao,Yan-Yu Wang,Bing-Bing Zhao,Gong-Wei Xiao,Yan-Qing Li,Xi Liu,Zhi-Lan Zou,Ye Yao,Hong Xiong

Stem cell research & therapy 9:140 PubMed29751772

2018

Expression profiling of cell-intrinsic regulators in the process of differentiation of human iPSCs into retinal lineages.

Applications

Species

Unspecified reactive species

Jen-Hua Chuang,Aliaksandr A Yarmishyn,De-Kuang Hwang,Chih-Chien Hsu,Mong-Lien Wang,Yi-Ping Yang,Ke-Hung Chien,Shih-Hwa Chiou,Chi-Hsien Peng,Shih-Jen Chen

Cell 173:470-484.e18 PubMed29551267

2018

B-Cell-Specific Diversion of Glucose Carbon Utilization Reveals a Unique Vulnerability in B Cell Malignancies.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Xiao,Lai N Chan,Lars Klemm,Daniel Braas,Zhengshan Chen,Huimin Geng,Qiuyi Chen Zhang,Ali Aghajanirefah,Kadriye Nehir Cosgun,Teresa Sadras,Jaewoong Lee,Tamara Mirzapoiazova,Ravi Salgia,Thomas Ernst,Andreas Hochhaus,Hassan Jumaa,Xiaoyan Jiang,David M Weinstock,Thomas G Graeber,Markus Müschen

Journal of immunology (Baltimore, Md. : 1950) 199:3504-3515 PubMed28978690

2017

Aryl Hydrocarbon Receptor Activation Suppresses EBF1 and PAX5 and Impairs Human B Lymphopoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinpeng Li,Sudin Bhattacharya,Jiajun Zhou,Ashwini S Phadnis-Moghe,Robert B Crawford,Norbert E Kaminski

The Journal of clinical investigation 127:987-1004 PubMed28192372

2017

RNA-binding protein PSPC1 promotes the differentiation-dependent nuclear export of adipocyte RNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Jiexin Wang,Prashant Rajbhandari,Andrey Damianov,Areum Han,Tamer Sallam,Hironori Waki,Claudio J Villanueva,Stephen D Lee,Ronni Nielsen,Susanne Mandrup,Karen Reue,Stephen G Young,Julian Whitelegge,Enrique Saez,Douglas L Black,Peter Tontonoz

PloS one 10:e0130495 PubMed26098938

2015

Retrovirus-Mediated Expression of E2A-PBX1 Blocks Lymphoid Fate but Permits Retention of Myeloid Potential in Early Hematopoietic Progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Mark W Woodcroft,Kyster Nanan,Patrick Thompson,Kathrin Tyryshkin,Steven P Smith,Robert K Slany,David P LeBrun

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EBF1 antibody [EPR4183]

Western blot - Anti-EBF1 antibody [EPR4183] (AB108369)

Western blot - Anti-EBF1 antibody [EPR4183] (AB108369)

Western blot - Anti-EBF1 antibody [EPR4183] (AB108369)

Western blot - Anti-EBF1 antibody [EPR4183] (AB108369)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise