Rabbit Recombinant Monoclonal EBF1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 8 publications.
Images
![Western blot - Anti-EBF1 antibody [EPR4183] (AB108369), expandable thumbnail](https://content.abcam.com/products/images/ebf1-antibody-epr4183-ab108369--western-blot-img58832.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-EBF1 antibody [EPR4183] (AB108369)")
![Western blot - Anti-EBF1 antibody [EPR4183] (AB108369), expandable thumbnail](https://content.abcam.com/products/images/ebf1-antibody-epr4183-ab108369--western-blot-img163174.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-EBF1 antibody [EPR4183] (AB108369)")
![Western blot - Anti-EBF1 antibody [EPR4183] (AB108369), expandable thumbnail](https://content.abcam.com/products/images/ebf1-antibody-epr4183-ab108369--western-blot-img126078.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-EBF1 antibody [EPR4183] (AB108369)")
Publications
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- Cells 11:2022Caveolin-1 rs1997623 Single Nucleotide Polymorphism Creates a New Binding Site for the Early B-Cell Factor 1 That Instigates Adipose Tissue CAV1 Protein Overexpression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshraf Al Madhoun,Dania Haddad,Rasheeba Nizam,Lavina Miranda,Shihab Kochumon,Reeby Thomas,Thangavel Alphonse Thanaraj,Rasheed Ahmad,Milad S Bitar,Fahd Al-MullaPubMed 36497195
- Clinical and translational medicine 11:e4792021Long noncoding RNA IL6-AS1 is highly expressed in chronic obstructive pulmonary disease and is associated with interleukin 6 by targeting miR-149-5p and early B-cell factor 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErkang Yi,Jiahuan Zhang,Mengning Zheng,Yi Zhang,Chunxiao Liang,Binwei Hao,Wei Hong,Biting Lin,Jinding Pu,Zhiwei Lin,Peiyu Huang,Bing Li,Yumin Zhou,Pixin RanPubMed 34323408
- Thyroid : official journal of the American Thyroid :2021Thyroid Hormone Receptor α1 Mutants Impair B Lymphocyte Development in a Mouse Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSunmi Park et. al.PubMed 33267733
- Stem cell research & therapy 9:1402018Expression profiling of cell-intrinsic regulators in the process of differentiation of human iPSCs into retinal lineages.
- Cell 173:470-484.e182018B-Cell-Specific Diversion of Glucose Carbon Utilization Reveals a Unique Vulnerability in B Cell Malignancies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGang Xiao et. al.PubMed 29551267
- Journal of immunology (Baltimore, Md. : 1950) 199:3504-35152017Aryl Hydrocarbon Receptor Activation Suppresses EBF1 and PAX5 and Impairs Human B Lymphopoiesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinpeng Li,Sudin Bhattacharya,Jiajun Zhou,Ashwini S Phadnis-Moghe,Robert B Crawford,Norbert E KaminskiPubMed 28978690
- The Journal of clinical investigation 127:987-10042017RNA-binding protein PSPC1 promotes the differentiation-dependent nuclear export of adipocyte RNAs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiexin Wang et. al.PubMed 28192372
- PloS one 10:e01304952015Retrovirus-Mediated Expression of E2A-PBX1 Blocks Lymphoid Fate but Permits Retention of Myeloid Potential in Early Hematopoietic Progenitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMark W Woodcroft et. al.PubMed 26098938
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | IHC-P | IP | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Tested | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000 | Notes For unpurified use at 1/10000. |
Species Rat | Dilution info 1/5000 | Notes For unpurified use at 1/10000. |
Species Human | Dilution info 1/5000 | Notes For unpurified use at 1/10000. |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Key pioneer transcription factor of B-cell specification and commitment (PubMed:27807034). Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Operates in a transcription factor network to activate B-cell-specific genes and repress genes associated with alternative cell fates. For instance, positively regulates many B-cell specific genes including BCR or CD40 while repressing genes that direct cells into alternative lineages, including GATA3 and TCF7 for the T-cell lineage. In addition to its role during lymphopoiesis, controls the thermogenic gene program in adipocytes during development and in response to environmental cold (By similarity).(Microbial infection) Acts as a chromatin anchor for Epstein-Barr virus EBNA2 to mediate the assembly of EBNA2 chromatin complexes in B-cells (PubMed:28968461). In addition, binds to the viral LMP1 proximal promoter and promotes its expression during latency (PubMed:26819314).
Alternative names
Transcription factor COE1, O/E-1, OE-1, Early B-cell factor, EBF, COE1, EBF1
Recommended products
Rabbit Recombinant Monoclonal EBF1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 8 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR4183
- Purification technique
Affinity purification Protein A
- Specificity
This antibody also recognises EBF3 protein.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-EBF1 antibody [EPR4183] (ab108369), expandable thumbnail](https://content.abcam.com/products/images/ebf1-antibody-epr4183-ab108369--western-blot-img58832.jpg "Western blot - Anti-EBF1 antibody [EPR4183] (ab108369)")
Western blot - Anti-EBF1 antibody [EPR4183] (ab108369)
We are unsure how to define the extra bands.
All lanes: Western blot - Anti-EBF1 antibody [EPR4183] (ab108369) at 1/1000 dilution
Lane 1: Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg
Lane 2: Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg
Lane 3: Mouse spleen lysate at 20 µg
Lane 4: Mouse lymph node lysate at 20 µg
Lane 5: Rat spleen lysate at 20 µg
Lane 6: Rat lymph node lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
![Western blot - Anti-EBF1 antibody [EPR4183] (ab108369), expandable thumbnail](https://content.abcam.com/products/images/ebf1-antibody-epr4183-ab108369--western-blot-img163174.jpg "Western blot - Anti-EBF1 antibody [EPR4183] (ab108369)")
Western blot - Anti-EBF1 antibody [EPR4183] (ab108369)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-EBF1 antibody [EPR4183] (ab108369) at 1/1000 dilution
All lanes: Raji cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 20 µg
Predicted band size: 64 kDa
![Western blot - Anti-EBF1 antibody [EPR4183] (ab108369), expandable thumbnail](https://content.abcam.com/products/images/ebf1-antibody-epr4183-ab108369--western-blot-img126078.jpg "Western blot - Anti-EBF1 antibody [EPR4183] (ab108369)")
Western blot - Anti-EBF1 antibody [EPR4183] (ab108369)
All lanes: Western blot - Anti-EBF1 antibody [EPR4183] (ab108369) at 1/1000 dilution
Lane 1: Raji cell lysate at 10 µg
Lane 2: Daudi cell lysate at 10 µg
Lane 3: 3T3-LI cell lysate at 10 µg
Predicted band size: 64 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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