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AB207705

Anti-EBF3 antibody

3

(1 Review)

|

(3 Publications)

Rabbit Polyclonal EBF3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EBF3 aa 250-450.

View Alternative Names

COE3, EBF3, Transcription factor COE3, Early B-cell factor 3, Olf-1/EBF-like 2, EBF-3, O/E-2, OE-2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBF3 antibody (AB207705)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBF3 antibody (AB207705)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human fetal muscle tissue labeling EBF3 with ab207705 at 1/100 dilution.

Western blot - Anti-EBF3 antibody (AB207705)
  • WB

Supplier Data

Western blot - Anti-EBF3 antibody (AB207705)

All lanes:

Western blot - Anti-EBF3 antibody (ab207705) at 1/1000 dilution

All lanes:

U87 cell lysate

Predicted band size: 65 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human EBF3 aa 250-450. The exact immunogen used to generate this antibody is proprietary information.

Q9H4W6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Early B-cell Factor 3 (EBF3) also known as COE3 (Collier/Olf/EBF) acts as a transcription factor important for B-cell development and neuronal differentiation. EBF3 with a mass of approximately 63 kDa is expressed in various tissues including the brain and bone marrow. It plays a role in DNA binding through its helix-loop-helix domain and is involved in the regulation of gene expression by interacting with specific promoter regions.
Biological function summary

EBF3 influences several critical cellular processes including neurogenesis and immune function. It contributes to the regulation of neuronal gene expression ensuring proper neuronal identity and function. EBF3 is part of a transcriptional complex with other EBF family members which collectively coordinate the expression of target genes essential for normal cellular differentiation and function.

Pathways

EBF3 integrates into the neurogenesis and immune response pathways. In the neurogenesis pathway it interacts with proteins such as NeuroD1 and Pax6 to influence the fate and differentiation of neural progenitors. Within the immune system EBF3 works alongside related proteins like PAX5 to regulate the development and function of B-cells maintaining immune homeostasis.

EBF3 has links to neurodevelopmental disorders and certain types of leukemia. Mutations or dysregulation of EBF3 can contribute to disorders such as hypotonia and ataxia linked to EBF3-related neurodevelopmental disorder (E3ND). In leukemia EBF3 can cooperate with abnormal signaling pathways to alter B-cell proliferation. In this context it may have interactions with proteins like RUNX1 influencing disease progression and therapeutic outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional activator (PubMed : 28017370, PubMed : 28017372, PubMed : 28017373). Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3' (By similarity).
See full target information EBF3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

JBMR plus 6:e10596 PubMed35309866

2022

A Subset of Osteosarcoma Bears Markers of CXCL12-Abundant Reticular Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Branden R Sosa,Ziqi Wang,John H Healey,Meera Hameed,Matthew B Greenblatt

British journal of haematology 193:659-668 PubMed33837967

2021

Identification of CXCL12-abundant reticular cells in human adult bone marrow.

Applications

Unspecified application

Species

Unspecified reactive species

Kazunari Aoki,Masako Kurashige,Michiko Ichii,Kei Higaki,Tatsuki Sugiyama,Takashi Kaito,Wataru Ando,Nobuhiko Sugano,Takashi Sakai,Hirohiko Shibayama,Akifumi Takaori-Kondo,Eiichi Morii,Yuzuru Kanakura,Takashi Nagasawa

Genes & development 32:359-372 PubMed29563184

2018

Stem cell niche-specific Ebf3 maintains the bone marrow cavity.

Applications

Unspecified application

Species

Unspecified reactive species

Masanari Seike,Yoshiki Omatsu,Hitomi Watanabe,Gen Kondoh,Takashi Nagasawa
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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