Rabbit Polyclonal EBF3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EBF3 aa 250-450.
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Publications
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- JBMR plus 6:e105962022A Subset of Osteosarcoma Bears Markers of CXCL12-Abundant Reticular Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBranden R Sosa,Ziqi Wang,John H Healey,Meera Hameed,Matthew B GreenblattPubMed 35309866
- British journal of haematology 193:659-6682021Identification of CXCL12-abundant reticular cells in human adult bone marrow.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKazunari Aoki,Masako Kurashige,Michiko Ichii,Kei Higaki,Tatsuki Sugiyama,Takashi Kaito,Wataru Ando,Nobuhiko Sugano,Takashi Sakai,Hirohiko Shibayama,Akifumi Takaori-Kondo,Eiichi Morii,Yuzuru Kanakura,Takashi NagasawaPubMed 33837967
- Genes & development 32:359-3722018Stem cell niche-specific Ebf3 maintains the bone marrow cavity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasanari Seike,Yoshiki Omatsu,Hitomi Watanabe,Gen Kondoh,Takashi NagasawaPubMed 29563184
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human EBF3 aa 250-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H4W6
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Xenopus laevis | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Xenopus laevis | Dilution info - | Notes - |
Target data
Function
Transcriptional activator (PubMed:28017370, PubMed:28017372, PubMed:28017373). Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3' (By similarity).
Alternative names
COE3, EBF3, Transcription factor COE3, Early B-cell factor 3, Olf-1/EBF-like 2, EBF-3, O/E-2, OE-2
Recommended products
Rabbit Polyclonal EBF3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EBF3 aa 250-450.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human EBF3 aa 250-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H4W6
- Purification technique
- Affinity purification Immunogen
- Reconstitution
- reconstitute with water at 200µL
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Early B-cell Factor 3 (EBF3) also known as COE3 (Collier/Olf/EBF) acts as a transcription factor important for B-cell development and neuronal differentiation. EBF3 with a mass of approximately 63 kDa is expressed in various tissues including the brain and bone marrow. It plays a role in DNA binding through its helix-loop-helix domain and is involved in the regulation of gene expression by interacting with specific promoter regions.
- Biological function summary
EBF3 influences several critical cellular processes including neurogenesis and immune function. It contributes to the regulation of neuronal gene expression ensuring proper neuronal identity and function. EBF3 is part of a transcriptional complex with other EBF family members which collectively coordinate the expression of target genes essential for normal cellular differentiation and function.
- Pathways
EBF3 integrates into the neurogenesis and immune response pathways. In the neurogenesis pathway it interacts with proteins such as NeuroD1 and Pax6 to influence the fate and differentiation of neural progenitors. Within the immune system EBF3 works alongside related proteins like PAX5 to regulate the development and function of B-cells maintaining immune homeostasis.
- Associated diseases and disorders
EBF3 has links to neurodevelopmental disorders and certain types of leukemia. Mutations or dysregulation of EBF3 can contribute to disorders such as hypotonia and ataxia linked to EBF3-related neurodevelopmental disorder (E3ND). In leukemia EBF3 can cooperate with abnormal signaling pathways to alter B-cell proliferation. In this context it may have interactions with proteins like RUNX1 influencing disease progression and therapeutic outcomes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-EBF3 antibody (ab207705)
All lanes: Western blot - Anti-EBF3 antibody (ab207705) at 1/1000 dilution
All lanes: U87 cell lysate
Predicted band size: 65 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBF3 antibody (ab207705)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human fetal muscle tissue labeling EBF3 with ab207705 at 1/100 dilution.
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