Anti-EBI3 antibody [EPR25341-155]

5 product images

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  Western blot - Anti-EBI3 antibody [EPR25341-155] (ab307195)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  

  Negative control: Jurkat (PMID: 11696437), MOLT-4 (PMID: 11696437).

  EBI3 is a glycoprotein of approximately 34 kDa and detected as a 27 kDa band after treated with Protein Deglycosylation MIX II.

  This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

  All lanes: Western blot - Anti-EBI3 antibody [EPR25341-155] (ab307195) at 1/1000 dilution

  Lane 1: Untreated HDLM-2 (human Hodgkin lymphoma) whole cell lysate 20 μg

  Lane 2: HDLM-2 whole cell lysate treated with Protein Deglycosylation Mix II 20 μg

  Lane 3: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate 20 μg

  Lane 4: MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate 20 μg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Developed using the ECL technique.

  Observed band size: 27 kDa, 34 kDa

  Exposure time: 180s

• 

  Western blot - Anti-EBI3 antibody [EPR25341-155] (ab307195)

  Blocking and diluting buffer and concentration: 5% NFDM/TBSTNegative control: human heart (PMID:8551575).
  
  The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 8551575).
  
  The identity of the higher MW band at approximately 50 kDa (in lane 1) is unknown.
  Exposure time: 26 seconds

  All lanes: Western blot - Anti-EBI3 antibody [EPR25341-155] (ab307195) at 1/1000 dilution

  Lane 1: Human placenta tissue lysate 20 μg

  Lane 2: Human heart tissue lysate 20 μg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 27 kDa, 34 kDa

  Exposure time: 26s

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [EPR25341-155] (ab307195)

  Immunohistochemical analysis of paraffin-embedded Human placenta tissue lABeling EBI3 with ab307195 at 1/2000 (0.26 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on human placenta (PMID:?11696437). The section was incubated with ab307195 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [EPR25341-155] (ab307195)

  Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue lABeling EBI3 with ab307195 at 1/2000 (0.26 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Negative control: No staining on human cardiac muscle. The section was incubated with ab307195 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Immunocytochemistry/ Immunofluorescence - Anti-EBI3 antibody [EPR25341-155] (ab307195)

  Immunofluorescent analysis of 80% Methanol-fixed, 0.1% TritonX-100 permeABilized HDLM-2 (human Hodgkin lymphoma cell) cells lABelling EBI3 with ab307195 at 1/50 (10.38 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in HDLM-2 cell line. Negative control: Jurkat (PMID: 15793300)Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/ml dilution.