Rabbit Recombinant Monoclonal EBI3 antibody. Carrier free. Suitable for WB, IP and reacts with Mouse samples.
Images
![Western blot - Anti-EBI3 antibody [EPR28427-89] - BSA and Azide free (AB316202), expandable thumbnail](https://content.abcam.com/products/images/ebi3-antibody-epr28427-89-bsa-and-azide-free-ab316202--western-blot-img426395.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-EBI3 antibody [EPR28427-89] - BSA and Azide free (AB316202)")
![Immunoprecipitation - Anti-EBI3 antibody [EPR28427-89] - BSA and Azide free (AB316202), expandable thumbnail](https://content.abcam.com/products/images/ebi3-antibody-epr28427-89-bsa-and-azide-free-ab316202--immunoprecipitation-img426417.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-EBI3 antibody [EPR28427-89] - BSA and Azide free (AB316202)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IP | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Target data
Function
Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.
Alternative names
Il27b, Ebi3, Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein homolog
Recommended products
Rabbit Recombinant Monoclonal EBI3 antibody. Carrier free. Suitable for WB, IP and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28427-89
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab316202 is the carrirer-free version of Anti-EBI3 antibody [EPR28427-89] ab316201.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EBI3 also known as Epstein-Barr virus induced 3 is a cytokine receptor protein with a molecular mass of approximately 34 kDa. This protein plays an important role in the immune system and is widely expressed in various tissues including spleen thymus and peripheral blood leukocytes. EBI3 is often found in cells involved in immune response such as macrophages and dendritic cells. It acts through the formation of heterodimers partnering with other proteins to exert its influence.
- Biological function summary
EBI3 serves a function as a component of the cytokine complex. It pairs with other interleukin proteins forming essential heterodimers like IL-27 in which it combines with the cytokine p28. This complex is involved in modulating the immune response including the suppression and activation of T-cells. Through its binding actions EBI3 influences the regulation of immune responses aiding in controlling inflammation and maintaining immune system balance.
- Pathways
EBI3 plays a critical role within the IL-27 signaling pathway. These pathways include the JAK/STAT signaling which is pivotal for mediating the immune response and influencing cell proliferation differentiation and apoptosis. EBI3 as part of the IL-27 complex interacts significantly with cytokines like IL-6 influencing the signal transduction pathways that are essential in immune cell communication and function.
- Associated diseases and disorders
EBI3 has associations with autoimmune diseases such as multiple sclerosis due to its role in regulating immune responses. Aberrant expression or function of EBI3 can contribute to the pathogenesis of chronic inflammatory diseases. It also holds potential links to cancer particularly in the modulation and development of tumor-induced immune evasion. In these disease contexts related proteins like p28 within the IL-27 complex interact with EBI3 affecting disease outcomes through their cooperative roles in immune modulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-EBI3 antibody [EPR28427-89] - BSA and Azide free (ab316202), expandable thumbnail](https://content.abcam.com/products/images/ebi3-antibody-epr28427-89-bsa-and-azide-free-ab316202--western-blot-img426395.jpg "Western blot - Anti-EBI3 antibody [EPR28427-89] - BSA and Azide free (ab316202)")
Western blot - Anti-EBI3 antibody [EPR28427-89] - BSA and Azide free (ab316202)
This data was developed using Anti-EBI3 antibody [EPR28427-89] ab316201, the same antibody clone in a different buffer formulation.
The up-regulation of EBI3 is induced by LPS treatment (PMID: 15728491).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-EBI3 antibody [EPR28427-89] (Anti-EBI3 antibody [EPR28427-89] ab316201) at 1/1000 dilution
Lane 1: Untreated RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: RAW 264.7 treated with /ml LPS for 4 hours, 1/ml BFA was then added for additional 3 hours, whole cell lysate µg at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 30 kDa, 36 kDa
Exposure time: 180s
![Immunoprecipitation - Anti-EBI3 antibody [EPR28427-89] - BSA and Azide free (ab316202), expandable thumbnail](https://content.abcam.com/products/images/ebi3-antibody-epr28427-89-bsa-and-azide-free-ab316202--immunoprecipitation-img426417.jpg "Immunoprecipitation - Anti-EBI3 antibody [EPR28427-89] - BSA and Azide free (ab316202)")
Immunoprecipitation - Anti-EBI3 antibody [EPR28427-89] - BSA and Azide free (ab316202)
This data was developed using Anti-EBI3 antibody [EPR28427-89] ab316201, the same antibody clone in a different buffer formulation.
EBI3 was immunoprecipitated from 0.35 mg RAW 264.7 treated with 100 ng/ml LPS for 4 hours, 1 ug/ml BFA was then added for additional 3 hours, whole cell lysate with Anti-EBI3 antibody [EPR28427-89] ab316201 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-EBI3 antibody [EPR28427-89] ab316201 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: RAW 264.7 treated with 100 ng/ml LPS for 4 hours, 1 ug/ml BFA was then added for additional 3 hours, whole cell lysate
Lane 2: Anti-EBI3 antibody [EPR28427-89] ab316201 IP in RAW 264.7 treated with 100 ng/ml LPS for 4 hours, 1 ug/ml BFA was then added for additional 3 hours, whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-EBI3 antibody [EPR28427-89] ab316201 in RAW 264.7 treated with 100 ng/ml LPS for 4 hours, 1 ug/ml BFA was then added for additional 3 hours, whole cell lysateAll lanes: Immunoprecipitation - Anti-EBI3 antibody [EPR28427-89] (Anti-EBI3 antibody [EPR28427-89] ab316201) at 1/30 dilution
All lanes: RAW 264.7 treated with 100 ng/ml LPS for 4 hours, 1 ug/ml BFA was then added for additional 3 hours, whole cell lysate with 5% NFDM/TBST
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 180s
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