Rabbit Recombinant Multiclonal EBI3 antibody. Carrier free. Suitable for IHC-P, WB, IP and reacts with Human, Mouse samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [RM2064] - BSA and Azide free (AB319169), expandable thumbnail](https://content.abcam.com/products/images/ebi3-antibody-rm2064-bsa-and-azide-free-ab319169--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img434056.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [RM2064] - BSA and Azide free (AB319169)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [RM2064] - BSA and Azide free (AB319169), expandable thumbnail](https://content.abcam.com/products/images/ebi3-antibody-rm2064-bsa-and-azide-free-ab319169--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img434055.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [RM2064] - BSA and Azide free (AB319169)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [RM2064] - BSA and Azide free (AB319169), expandable thumbnail](https://content.abcam.com/products/images/ebi3-antibody-rm2064-bsa-and-azide-free-ab319169--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img434054.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [RM2064] - BSA and Azide free (AB319169)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [RM2064] - BSA and Azide free (AB319169), expandable thumbnail](https://content.abcam.com/products/images/ebi3-antibody-rm2064-bsa-and-azide-free-ab319169--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img434053.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [RM2064] - BSA and Azide free (AB319169)")
![Western blot - Anti-EBI3 antibody [RM2064] - BSA and Azide free (AB319169), expandable thumbnail](https://content.abcam.com/products/images/ebi3-antibody-rm2064-bsa-and-azide-free-ab319169--western-blot-img434113.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-EBI3 antibody [RM2064] - BSA and Azide free (AB319169)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | IP | Flow Cyt (Intra) | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.
Additional Targets
EBI3
Alternative names
Il27b, Ebi3, Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein homolog
Recommended products
Rabbit Recombinant Multiclonal EBI3 antibody. Carrier free. Suitable for IHC-P, WB, IP and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- RM2064
- Purification technique
- Affinity purification Protein A
- Specificity
Unsuitable for Mouse IP and IHC-P.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab319169 is the carrier-free version of Anti-EBI3 antibody [RM2064] ab319168.
This product is a recombinant multiclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EBI3 also known as Epstein-Barr virus induced 3 is a cytokine receptor protein with a molecular mass of approximately 34 kDa. This protein plays an important role in the immune system and is widely expressed in various tissues including spleen thymus and peripheral blood leukocytes. EBI3 is often found in cells involved in immune response such as macrophages and dendritic cells. It acts through the formation of heterodimers partnering with other proteins to exert its influence.
- Biological function summary
EBI3 serves a function as a component of the cytokine complex. It pairs with other interleukin proteins forming essential heterodimers like IL-27 in which it combines with the cytokine p28. This complex is involved in modulating the immune response including the suppression and activation of T-cells. Through its binding actions EBI3 influences the regulation of immune responses aiding in controlling inflammation and maintaining immune system balance.
- Pathways
EBI3 plays a critical role within the IL-27 signaling pathway. These pathways include the JAK/STAT signaling which is pivotal for mediating the immune response and influencing cell proliferation differentiation and apoptosis. EBI3 as part of the IL-27 complex interacts significantly with cytokines like IL-6 influencing the signal transduction pathways that are essential in immune cell communication and function.
- Associated diseases and disorders
EBI3 has associations with autoimmune diseases such as multiple sclerosis due to its role in regulating immune responses. Aberrant expression or function of EBI3 can contribute to the pathogenesis of chronic inflammatory diseases. It also holds potential links to cancer particularly in the modulation and development of tumor-induced immune evasion. In these disease contexts related proteins like p28 within the IL-27 complex interact with EBI3 affecting disease outcomes through their cooperative roles in immune modulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169), expandable thumbnail](https://content.abcam.com/products/images/ebi3-antibody-rm2064-bsa-and-azide-free-ab319169--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img434056.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169)
This data was developed using Anti-EBI3 antibody [RM2064] ab319168, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling EBI3 with Anti-EBI3 antibody [RM2064] ab319168 at 1/500 (0.978 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
MSD2027: No staining on human skeletal muscle.
The section was incubated with Anti-EBI3 antibody [RM2064] ab319168 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
MSD279 antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169), expandable thumbnail](https://content.abcam.com/products/images/ebi3-antibody-rm2064-bsa-and-azide-free-ab319169--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img434055.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169)
This data was developed using Anti-EBI3 antibody [RM2064] ab319168, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling EBI3 with Anti-EBI3 antibody [RM2064] ab319168 at 1/500 (0.978 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
MSD2027: No staining on human cardiac muscle.
The section was incubated with Anti-EBI3 antibody [RM2064] ab319168 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
MSD279 antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169), expandable thumbnail](https://content.abcam.com/products/images/ebi3-antibody-rm2064-bsa-and-azide-free-ab319169--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img434054.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169)
This data was developed using Anti-EBI3 antibody [RM2064] ab319168, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling EBI3 with Anti-EBI3 antibody [RM2064] ab319168 at 1/500 (0.978 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human tonsil (PMID: 8551575).
The section was incubated with Anti-EBI3 antibody [RM2064] ab319168 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
MSD279 antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169), expandable thumbnail](https://content.abcam.com/products/images/ebi3-antibody-rm2064-bsa-and-azide-free-ab319169--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img434053.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169)
This data was developed using Anti-EBI3 antibody [RM2064] ab319168, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling EBI3 with Anti-EBI3 antibody [RM2064] ab319168 at 1/500 (0.978 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human placenta (PMID: 8551575).
The section was incubated with Anti-EBI3 antibody [RM2064] ab319168 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
MSD279 antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Western blot - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169), expandable thumbnail](https://content.abcam.com/products/images/ebi3-antibody-rm2064-bsa-and-azide-free-ab319169--western-blot-img434113.jpg "Western blot - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169)")
Western blot - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169)
The up-regulation of EBI3 is induced by LPS treatment (PMID: 15728491).
The band nearby 75 kDa could be non-specific band.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Blocking and dilution buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-EBI3 antibody [RM2064] (Anti-EBI3 antibody [RM2064] ab319168) at 1/1000 dilution
Lane 1: Untreated RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 2: RAW 264.7 treated with /ml LPS for 4 hours, 1/ml BFA was then added for additional 3 hours, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 34 kDa, 36 kDa
Exposure time: 180s
![Western blot - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169), expandable thumbnail](https://content.abcam.com/products/images/ebi3-antibody-rm2064-bsa-and-azide-free-ab319169--western-blot-img434112.jpg "Western blot - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169)")
Western blot - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169)
Negative control: Jurkat and MOLT-4 (PMID: 11696437), skeletal muscle(PMID: 21849417)
EBI3 is a glycoprotein of approximately 34 kDa and detected as a 27 kDa band after treated with Protein Deglycosylation MIX II(PMID: 8551575).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Blocking and dilution buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-EBI3 antibody [RM2064] (Anti-EBI3 antibody [RM2064] ab319168) at 1/1000 dilution
Lane 1: Untreated HDLM-2 (human Hodgkin lymphoma) whole cell lysate at 20 µg
Lane 2: HDLM-2 whole cell lysate treated with Protein Deglycosylation Mix II at 20 µg
Lane 3: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg
Lane 4: MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg
Lane 5: Human placenta tissue lysate at 20 µg
Lane 6: Human skeletal muscle tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 27 kDa, 34 kDa, 36 kDa
Exposure time: 180s
![Immunoprecipitation - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169), expandable thumbnail](https://content.abcam.com/products/images/ebi3-antibody-rm2064-bsa-and-azide-free-ab319169--immunoprecipitation-img434084.jpg "Immunoprecipitation - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169)")
Immunoprecipitation - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169)
This data was developed using Anti-EBI3 antibody [RM2064] ab319168, the same antibody clone in a different buffer formulation.
EBI3 was immunoprecipitated from 0.35 mg HDLM-2 (human Hodgkin lymphoma) whole cell lysate with Anti-EBI3 antibody [RM2064] ab319168 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-EBI3 antibody [RM2064] ab319168 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HDLM-2 (human Hodgkin lymphoma) whole cell lysate
Lane 2: Anti-EBI3 antibody [RM2064] ab319168 IP in HDLM-2 (human Hodgkin lymphoma) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-EBI3 antibody [RM2064] ab319168 in HDLM-2 whole cell lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST
All lanes: Immunoprecipitation - Anti-EBI3 antibody [RM2064] (Anti-EBI3 antibody [RM2064] ab319168) at 1/30 dilution
All lanes: HDLM-2 (human Hodgkin lymphoma) whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 180s
![Western blot - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169), expandable thumbnail](https://content.abcam.com/products/images/ebi3-antibody-rm2064-bsa-and-azide-free-ab319169--western-blot-img434112.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169)")
![Immunoprecipitation - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169), expandable thumbnail](https://content.abcam.com/products/images/ebi3-antibody-rm2064-bsa-and-azide-free-ab319169--immunoprecipitation-img434084.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-EBI3 antibody [RM2064] - BSA and Azide free (ab319169)")
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