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AB16128

Anti-EBNA 3C antibody

3

(1 Review)

|

(9 Publications)

Sheep Polyclonal EBNA6 antibody. Suitable for ELISA, ICC/IF, IHC-Fr and reacts with Epstein-Barr virus samples. Cited in 9 publications.

View Alternative Names

EBNA 4B, EBV, EBV nuclear antigen 6, Epstein Barr nuclear antigen 6, HHV4, Human Herpesvirus 4

Key facts

Host species

Sheep

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Epstein-Barr virus

Applications

ICC/IF, IHC-Fr, ELISA

applications

Specificity

This antibody is specific for the Epstein Barr Virus Nuclear Antigen 3C.

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Reactivity data

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Product details

EBNA 3C is a latent viral nuclear protein expressed in EBV transformed lymphoblastic cell lines and is essential for EBV mediated transformation of these cell lines. This viral nuclear protein is essential for EBV mediated positive immunoblastic lymphomas, and functions as a transcription factor.

Source: Sheep were immunized with recombinant EBNA-3C protein

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide.

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Properties and storage information

Form
Liquid
Purification technique
Precipitation Ammonium Sulphate
Purification notes
Purified by ammonium sulfate precipitationThis antibody is provided as a 0.2µm sterile filtered solution.
Storage buffer
Preservative: 0.08% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EBNA 3C or Epstein-Barr virus nuclear antigen 3C is a significant protein encoded by the Epstein-Barr virus. Weighing approximately 100 kDa EBNA 3C operates within infected B cells. It plays an important role in transcriptional regulation interacting with various cellular proteins to modify gene expression. The protein recruits histone modification enzymes influencing chromatin structure and affecting transcriptional activity. EBNA 3C is often associated with the modulation of host cell cycle progression and can influence cell proliferation.
Biological function summary

The EBNA 3C protein contributes to the transformation of infected B cells into immortalized cells a hallmark of latent Epstein-Barr virus infection. This protein is part of an EBNAs family complex that includes EBNA 1 EBNA 2 and EBNA LP working together to alter host cell functions to the virus’s advantage. EBNA 3C has a role in the proteasome-dependent degradation of certain cellular proteins impacting cell cycle checkpoints and DNA repair mechanisms.

Pathways

EBNA 3C interacts with pathways that regulate cell cycle and apoptosis. The protein interrupts the retinoblastoma (Rb) protein pathway by degrading Rb-associated proteins allowing cell cycle progression independent of regular controls. Additionally the NF-kB signaling pathway where EBNA 3C acts enhances survival and proliferation signals in infected cells. The protein partnerships in these pathways include p53 and cyclin D1 which receive modulation of their functions by EBNA 3C to favor viral persistence.

EBNA 3C has associations with various Epstein-Barr virus-related cancers such as Burkitt's lymphoma and nasopharyngeal carcinoma. In these diseases EBNA 3C modulates oncogenic pathways supporting uncontrolled cell growth. The protein's interaction with p27 which regulates cell cycle progression further connects EBNA 3C to oncogenic processes. Targeting EBNA 3C expression and activity continues as a focus in developing therapeutic strategies against Epstein-Barr virus-associated malignancies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information EBNA 3C
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 6: PubMed36878637

2023

Endemic Burkitt lymphoma avatar mouse models for exploring inter-patient tumor variation and testing targeted therapies.

Applications

Unspecified application

Species

Unspecified reactive species

Priya Saikumar Lakshmi,Cliff I Oduor,Catherine S Forconi,Viriato M'Bana,Courtney Bly,Rachel M Gerstein,Juliana A Otieno,John M Ong'echa,Christian Münz,Micah A Luftig,Michael A Brehm,Jeffrey A Bailey,Ann M Moormann

PLoS pathogens 16:e1008105 PubMed32092124

2020

Proteasomal inhibition triggers viral oncoprotein degradation via autophagy-lysosomal pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chandrima Gain,Samaresh Malik,Shaoni Bhattacharjee,Arijit Ghosh,Erle S Robertson,Benu Brata Das,Abhik Saha

PLoS pathogens 15:e1008030 PubMed31518366

2019

Epstein-Barr virus subverts mevalonate and fatty acid pathways to promote infected B-cell proliferation and survival.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Wei Wang,Zhonghao Wang,Ina Ersing,Luis Nobre,Rui Guo,Sizun Jiang,Stephen Trudeau,Bo Zhao,Michael P Weekes,Benjamin E Gewurz

Journal of virology 92: PubMed30135119

2018

Epstein-Barr Virus Nuclear Antigen 3C Inhibits Expression of and the Locus via Interaction with the Histone Lysine Demethylase KDM2B.

Applications

Unspecified application

Species

Unspecified reactive species

Adam C T Gillman,Gillian Parker,Martin J Allday,Quentin Bazot

Cell death & disease 9:605 PubMed29789559

2018

Transcriptional and epigenetic modulation of autophagy promotes EBV oncoprotein EBNA3C induced B-cell survival.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoni Bhattacharjee,Priyanka Bose,Krishna Patel,Shatadru Ghosh Roy,Chandrima Gain,Harsha Gowda,Erle S Robertson,Abhik Saha

PLoS pathogens 10:e1004125 PubMed24809689

2014

Epstein-Barr virus down-regulates tumor suppressor DOK1 expression.

Applications

WB

Species

Unspecified reactive species

Maha Siouda,Cecilia Frecha,Rosita Accardi,Jiping Yue,Cyrille Cuenin,Henri Gruffat,Evelyne Manet,Zdenko Herceg,Bakary S Sylla,Massimo Tommasino

PLoS pathogens 9:e1003636 PubMed24068937

2013

Modulation of enhancer looping and differential gene targeting by Epstein-Barr virus transcription factors directs cellular reprogramming.

Applications

ChIP-seq

Species

Unspecified reactive species

Michael J McClellan,C David Wood,Opeoluwa Ojeniyi,Tim J Cooper,Aditi Kanhere,Aaron Arvey,Helen M Webb,Richard D Palermo,Marie L Harth-Hertle,Bettina Kempkes,Richard G Jenner,Michelle J West

Journal of virology 86:5165-78 PubMed22357270

2012

Downregulation of integrin receptor-signaling genes by Epstein-Barr virus EBNA 3C via promoter-proximal and -distal binding elements.

Applications

ChIP

Species

Unspecified reactive species

Michael J McClellan,Sarika Khasnis,C David Wood,Richard D Palermo,Sandra N Schlick,Aditi S Kanhere,Richard G Jenner,Michelle J West

Proceedings of the National Academy of Sciences of 107:3146-51 PubMed20133771

2010

Hsp90 inhibitors block outgrowth of EBV-infected malignant cells in vitro and in vivo through an EBNA1-dependent mechanism.

Applications

WB

Species

Unspecified reactive species

Xiaoping Sun,Elizabeth A Barlow,Shidong Ma,Stacy R Hagemeier,Sarah J Duellman,Richard R Burgess,Judy Tellam,Rajiv Khanna,Shannon C Kenney
View all publications
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