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AB90543

Anti-EBNA2 antibody [PE2]

5

(2 Reviews)

|

(35 Publications)

Anti-EBNA2 antibody [PE2] (ab90543) is a mouse monoclonal antibody detecting EBNA2 in Western Blot, IP, IHC-Fr, ICC/IF. Suitable for Epstein-Barr virus.

- Over 30 publications
- Trusted since 2010

View Alternative Names

BYRF1, EBNA2, Epstein-Barr nuclear antigen 2, EBNA-2, EBV nuclear antigen 2

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

PE2

Isotype

IgG1

Carrier free

No

Reacts with

Epstein-Barr virus

Applications

IP, IHC-Fr, ICC/IF, WB

applications

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Reactivity data

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Product details

Product Specifications
Anti-EBNA2 antibody [PE2] (ab90543) is a mouse monoclonal antibody and is validated for use in ICC/IF, IHC-Fr, IP, WB in Epstein-Barr virus samples.
Anti-EBNA2 antibody [PE2] (ab90543) specifically detects EBNA2 (UniProt ID: Q69022; Molecular weight: 49kDa) and is sold in 100µg selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-EBNA2 antibody [PE2] (ab90543) has high sensitivity and specificity.
Anti-EBNA2 antibody [PE2] (ab90543) has been cited over 37 times in peer reviewed journals and is trusted by the scientific community.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: 99.98% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EBNA2 also known as the Epstein-Barr virus nuclear antigen 2 (EBNA-2) plays a critical role in viral protein function. It is a transcriptional activator essential for the latent infection cycle of Epstein-Barr virus (EBV). EBNA2 binds to specific DNA sequences and interacts with other host cellular factors to initiate transcription. The molecular mass of EBNA2 typically is approximately 88 kDa. EBNA2 mainly expresses in EBV-infected B lymphocytes where it contributes to their immortalization and proliferation.
Biological function summary

EBNA2 is pivotal in the activation of several viral and cellular genes. It forms complexes with other proteins including the cellular transcription factor RBP-Jκ part of the Notch signaling pathway. This interaction is important for altering the expression patterns of both host and viral genes causing changes in the cell cycle and promoting latency. EBNA2 also regulates genes involved in immune response modulation aiding in viral persistence within the host by evading immune surveillance.

Pathways

EBNA2 impacts transcriptional regulation and cell signaling reversibly. It is integral to the Notch signaling and transcriptional activation pathway related to EBV latency. EBNA2 recruits proteins like CBF1 (RBP-Jκ) a transcription factor that helps mediate cell fate decisions and maintain latency. It modulates pathways involving c-MYC a central regulator of cell growth and influences transcriptional activity with a downstream effect on cellular transformation and proliferation.

EBNA2 is prominently associated with EBV-related malignancies such as Burkitt's lymphoma and Hodgkin's lymphoma. These cancers arise partly from the dysregulated cellular growth induced by EBNA2-mediated transcriptional changes. In these disorders EBNA2 may interact with proteins like LMP1 another EBV-encoded protein that aids in the transformation process promoting oncogenesis and affecting therapy resistance mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a key role in the activation of the host resting B-cell and stimulation of B-cell proliferation. Acts by up-regulating the expression of viral EBNA1-6, LMP1, LMP2A and LMP2B genes, as well as several host genes including CD21, CD23 and MYC. Activates transcription by acting as an adapter molecule that binds to cellular sequence-specific DNA-binding proteins such as host CBF1, SMARCB1 and SPI1. Once EBNA2 is near promoter sites, its acidic activating domain recruits basal and activation-associated transcription factors TFIIB, TAF40, TFIIH components ERCC2 and ERCC3, and CBP in order to promote transcription. Alternatively, EBNA2 can affect activities of cell cycle regulators and retard cell cycle progression at G2/M phase. It also induces chromosomal instability, by disrupting mitotic checkpoints, multi-nucleation and formation of micronuclei in infected cells.
See full target information EBNA2
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Publications (35)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 13:1363-1376 PubMed37168348

2023

EBNA2 mediates lipid metabolism and tumorigenesis through activation of ATF4 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Feng,Ping Zhang,Paul Yao,Hongyu Zhang

Life science alliance 6: PubMed36878637

2023

Endemic Burkitt lymphoma avatar mouse models for exploring inter-patient tumor variation and testing targeted therapies.

Applications

Unspecified application

Species

Unspecified reactive species

Priya Saikumar Lakshmi,Cliff I Oduor,Catherine S Forconi,Viriato M'Bana,Courtney Bly,Rachel M Gerstein,Juliana A Otieno,John M Ong'echa,Christian Münz,Micah A Luftig,Michael A Brehm,Jeffrey A Bailey,Ann M Moormann

mBio 14:e0345922 PubMed36786572

2023

Epstein-Barr Virus-Positive Lymphomas Exploit Ectonucleotidase Activity To Limit Immune Responses and Prevent Cell Death.

Applications

Unspecified application

Species

Unspecified reactive species

Philip T Lange,Blossom Damania

PLoS pathogens 18:e1010868 PubMed36190982

2022

Type 1 and Type 2 Epstein-Barr viruses induce proliferation, and inhibit differentiation, in infected telomerase-immortalized normal oral keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Deo R Singh,Scott E Nelson,Abigail S Pawelski,Juan A Cantres-Velez,Alisha S Kansra,Nicholas P Pauly,Jillian A Bristol,Mitchell Hayes,Makoto Ohashi,Alejandro Casco,Denis Lee,Stuart A Fogarty,Paul F Lambert,Eric C Johannsen,Shannon C Kenney

PLoS pathogens 18:e1010400 PubMed35421198

2022

The nuclear lamina binds the EBV genome during latency and regulates viral gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa Beatrice Caruso,Rui Guo,Kelsey Keith,Jozef Madzo,Davide Maestri,Sarah Boyle,Jason Wasserman,Andrew Kossenkov,Benjamin E Gewurz,Italo Tempera

International journal of molecular sciences 23: PubMed35408790

2022

Expression of the Chemokine Receptor CCR1 in Burkitt Lymphoma Cell Lines Is Linked to the CD10-Negative Cell Phenotype and Co-Expression of the EBV Latent Genes EBNA2, LMP1, and LMP2.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Zvejniece,Svetlana Kozireva,Zanna Rudevica,Ainars Leonciks,Barbro Ehlin-Henriksson,Elena Kashuba,Irina Kholodnyuk

Cancer immunology, immunotherapy : CII 71:1371-1392 PubMed34668039

2021

CD4 + T cells are found within endemic Burkitt lymphoma and modulate Burkitt lymphoma precursor cell viability and expression of pathogenically relevant Epstein-Barr virus genes.

Applications

Unspecified application

Species

Unspecified reactive species

Semjon Sidorov,Lara Fux,Katja Steiner,Samyo Bounlom,Sabrina Traxel,Tarik Azzi,Arbeneshe Berisha,Christoph Berger,Michele Bernasconi,Felix K Niggli,Yvonne Perner,Sugeshnee Pather,Werner Kempf,David Nadal,Simone Bürgler

Cell reports 35:109056 PubMed33951431

2021

KSHV infection drives poorly cytotoxic CD56-negative natural killer cell differentiation in vivo upon KSHV/EBV dual infection.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole Caduff,Donal McHugh,Lisa Rieble,Catherine S Forconi,John M Ong'echa,Peter O Oluoch,Ana Raykova,Anita Murer,Michelle Böni,Lara Zuppiger,Thomas F Schulz,David J Blackbourn,Obinna Chijioke,Ann M Moormann,Christian Münz

Frontiers in oncology 11:626659 PubMed33898307

2021

Characterization and Establishment of a Novel EBV Strain Simultaneously Associated With Nasopharyngeal Carcinoma and B-Cell Lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fenggang Yu,Nicholas L Syn,Yanan Lu,Qing Yun Chong,Junyun Lai,Wei Jian Tan,Boon Cher Goh,Paul A MacAry,Lingzhi Wang,Kwok Seng Loh

Experimental neurology 336:113537 PubMed33259808

2020

Intrauterine growth restriction compromises cerebellar development by affecting radial migration of granule cells via the JamC/Pard3a molecular pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Igor Y Iskusnykh,Nikolai Fattakhov,Randal K Buddington,Victor V Chizhikov
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
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(0 reviews)

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