Anti-EBNA2 antibody [PE2]

• WB

CiteAb

Western blot - Anti-EBNA2 antibody [PE2] (AB90543)

EBNA2 western blot using Anti-EBNA2 antibody [PE2] ab90543. Publication image and figure legend from Zvejniece, L., Kozireva, S., et al., 2022, International Journal of Molecular Sciences PubMed 35408790.

Phenotype of the Burkitt lymphoma cell lines. The percentages (%) of CD10-positive (A) and CCR1-expressing (B) cells were obtained using flow cytometry analysis. Annexin V-stained (apoptotic) and autofluorescent cells were omitted from the analysis. The presented data are from at least two independent experiments and are shown as the mean with the SD. (C) The IGHV gene identity (%) to the top germline genes was defined using the NCBI IGBLAST Tool. (D) EBNA2 and LMP1 protein expression in the type II and type III BL cell lines was assessed using immunoblotting of cell lysates and specific antibodies; the human beta-actin protein was probed as the loading control. The Raji and Mutu III BL type II cell lines served as the positive control (Table S1). All clones of the Mutu cell line are derivatives of an early-passage BL cell line [14]. BL41 is an EBV-negative cell line. A small panel, the same immunoblot with prolonged exposure, shows the EBNA2 protein in the cell lines BL41/95, BL16, Akuba, and Raji. (E) Immunofluorescent staining of CCR1 is shown in cells of the type III BL cell line Mutu cl.99 and human myeloid histiocytic lymphoma cell line U937, used as the positive control; in DG75, the EBV and the CCR1 transcript negative BL cell line, the CCR1 immunofluorescent signal was not observed.

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