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AB327600

Anti-Ebola virus VP40 antibody [HL4648] - BSA and Azide free

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Rabbit Recombinant Monoclonal VP40 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - Ebola virus - Mayinga, Zaire, 1976, Transfected cell line - Ebola virus - Mayinga, Zaire, 1976 samples.

View Alternative Names

Matrix protein VP40, Ebola VP40, Membrane-associated protein VP40, eVP40

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Ebola virus VP40 antibody [HL4648] - BSA and Azide free (AB327600)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ebola virus VP40 antibody [HL4648] - BSA and Azide free (AB327600)

ab327600 detects Ebola virus VP40 protein by immunofluorescent analysis.
Sample : Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green : Ebola virus VP40 stained by ab327600 diluted at 1 : 500.
Blue : Fluoroshield with DAPI.

Western blot - Anti-Ebola virus VP40 antibody [HL4648] - BSA and Azide free (AB327600)
  • WB

Supplier Data

Western blot - Anti-Ebola virus VP40 antibody [HL4648] - BSA and Azide free (AB327600)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ab327600 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Ebola virus VP40 antibody [HL4648] - BSA and Azide free (ab327600) at 1/5000 dilution

Lane 1:

Non-transfected 293T cells at 30 µg

Lane 2:

DDDDK-tagged Zaire ebolavirus VP40 transfected 293T cells at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL4648

Isotype

IgG

Carrier free

Yes

Reacts with

Ebola virus - Mayinga, Zaire, 1976

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Ebola virus - Mayinga, Zaire, 1976": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Ebola virus - Mayinga, Zaire, 1976": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Transfected cell lysate - Ebola virus - Mayinga, Zaire, 1976": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an essential role virus particle assembly and budding (PubMed : 16719918, PubMed : 33673144). Acts by interacting with viral ribonucleocapsid and host members of the ESCRT (endosomal sorting complex required for transport) system such as host VPS4, PDCD6IP/ALIX, NEDD4 or TGS101 (PubMed : 15892969, PubMed : 16719918, PubMed : 23637409, PubMed : 25786915, PubMed : 26753796, PubMed : 27489272). The interaction with host E3 ubiquitin ligase SMURF2 also facilitates virus budding (PubMed : 33673144). May play a role in immune cell dysfunction by being packaged into exosomes that can decrease the viability of recipient cells (via RNAi suppression and exosome-bystander apoptosis) (PubMed : 27872619).
See full target information VP40
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com