AB180602

Anti-EBP1 antibody [EPR14569(B)]

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal EBP1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 5 publications.

View Alternative Names

EBP1, PA2G4, Proliferation-associated protein 2G4, Cell cycle protein p38-2G4 homolog, ErbB3-binding protein 1, hG4-1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBP1 antibody [EPR14569(B)] (AB180602)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBP1 antibody [EPR14569(B)] (AB180602)

Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labeling EBP1 with ab180602 at 1/100 dilution, followed by prediluted ImmunoHistoprobe HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-EBP1 antibody [EPR14569(B)] (AB180602)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-EBP1 antibody [EPR14569(B)] (AB180602)

Intracellular flow cytometric analysis of2% paraformaldehyde-fixedJurkat cells labeling EBP1 with ab180602 at 1/20 dilution (red) compared to a Rabbit monoclonal IgG Isotypecontrol (green), followed byGoat anti rabbit IgG (FITC)at 1/150 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-EBP1 antibody [EPR14569(B)] (AB180602)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EBP1 antibody [EPR14569(B)] (AB180602)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling EBP1 with 180602 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution (Left panel). Right panel : Dapi staining.

Supplier Data

Western blot - Anti-EBP1 antibody [EPR14569(B)] (AB180602)

Blocking buffer and concentration : 5% NFDM/TBST. Diluting buffer and concentration : 5% NFDM/TBST. ab92554 recognizes human Cul4A and Cul4B overexpression vectors. ab180602 was used as a loading control.

All lanes:

Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (<a href='/en-us/products/primary-antibodies/cullin-4a-cul-4a-antibody-epr3198-ab92554'>ab92554</a>) at 1/10000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) transfected with blank vectors whole cell lysate at 20 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) transfected with human full length Cul4B expression vectors whole cell lysate at 20 µg

Lane 3:

HEK-293 (Human embryonic kidney epithelial cell) transfected with human full length Cul4A expression vectors whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 88 kDa

Observed band size: 88 kDa,104 kDa

false

Exposure time: 1s

Supplier Data

Western blot - Anti-EBP1 antibody [EPR14569(B)] (AB180602)

All lanes:

Western blot - Anti-EBP1 antibody [EPR14569(B)] (ab180602) at 1/20000 dilution

Lane 1:

293T lysate at 20 µg

Lane 2:

HeLa lysate at 20 µg

Lane 3:

Jurkat lysate at 20 µg

Lane 4:

A431 lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 44 kDa

Observed band size: 48 kDa

false

Carrier free

Anti-EBP1 antibody [EPR14569(B)] - BSA and Azide free
660 APC

APC Anti-EBP1 antibody [EPR14569(B)]
HRP

HRP Anti-EBP1 antibody [EPR14569(B)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-EBP1 antibody [EPR14569(B)]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-EBP1 antibody [EPR14569(B)]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-EBP1 antibody [EPR14569(B)]
578 PE

PE Anti-EBP1 antibody [EPR14569(B)]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-EBP1 antibody [EPR14569(B)]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-EBP1 antibody [EPR14569(B)]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-EBP1 antibody [EPR14569(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14569(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The EBP1 protein also known as ErbB3-binding protein 1 plays an important role in cellular mechanisms. With a mass of about 39 kDa EBP1 is widely expressed in various tissues including the heart liver and brain. The protein acts as an RNA-binding protein and it has demonstrated influence in the regulation of ribosomal RNA synthesis and processing. EBP1 also interacts with multiple other proteins which aid in its regulatory roles in the cells.

Biological function summary

EBP1 functions as a part of the cellular machinery involved in growth and differentiation. It associates with the eukaryotic translation initiation factor 4G (eIF4G) as part of a complex which modulates the translation of certain messenger RNAs. Furthermore EBP1 can function as a transcriptional co-repressor in the nucleus where it influences gene expression by interacting with DNA. Its activity in these roles highlights its important function in cell cycle regulation and cellular proliferation.

Pathways

Many signaling mechanisms engage EBP1. The PI3K/AKT pathway sees participation from EBP1 where it downstream affects cell survival and growth. EBP1 also interacts with the NF-κB pathway contributing to the regulation of immune and stress responses. In these pathways interactions with key proteins like AKT and NF-κB p65 emphasize its role in integrating signaling cues for responses to environmental stimuli.

EBP1 has shown associations with cancer and neurodegenerative diseases. Its connection with cancer is evident as EBP1 influences cell proliferation and can affect tumor suppressor pathways sometimes interacting with proteins like HER2. In neurodegenerative diseases deregulation of EBP1 function has been seen in conditions such as Alzheimer's potentially interacting with proteins involved in tau phosphorylation. Both examples demonstrate the biological significance of EBP1 in maintaining normal physiological function and its contributions to pathology when dysregulated.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site) (By similarity). Regulates cell proliferation, differentiation, and survival. Isoform 1 suppresses apoptosis whereas isoform 2 promotes cell differentiation (By similarity).

See full target information PA2G4

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:4739 PubMed38834613

2024

Orthogonal proteogenomic analysis identifies the druggable PA2G4-MYC axis in 3q26 AML.

Applications

Unspecified application

Species

Unspecified reactive species

Matteo Marchesini,Andrea Gherli,Elisa Simoncini,Lucas Moron Dalla Tor,Anna Montanaro,Natthakan Thongon,Federica Vento,Chiara Liverani,Elisa Cerretani,Anna D'Antuono,Luca Pagliaro,Raffaella Zamponi,Chiara Spadazzi,Elena Follini,Benedetta Cambò,Mariateresa Giaimo,Angela Falco,Gabriella Sammarelli,Giannalisa Todaro,Sabrina Bonomini,Valentina Adami,Silvano Piazza,Claudia Corbo,Bruno Lorusso,Federica Mezzasoma,Costanza Anna Maria Lagrasta,Maria Paola Martelli,Roberta La Starza,Antonio Cuneo,Franco Aversa,Cristina Mecucci,Federico Quaini,Simona Colla,Giovanni Roti

Pharmaceuticals (Basel, Switzerland) 16: PubMed37765033

2023

Effect of Repeated Administration of ɣ-Valerolactone (GVL) and GHB in the Mouse: Neuroadaptive Changes of the GHB and GABAergic System.

Applications

Unspecified application

Species

Unspecified reactive species

Paolo Frisoni,Giorgia Corli,Sabrine Bilel,Micaela Tirri,Laura Camilla Gasparini,Letizia Alfieri,Margherita Neri,Fabio De-Giorgio,Matteo Marti

The Tohoku journal of experimental medicine 259:199-208 PubMed36517015

2022

MiR-423-5p Inhibition Exerts Protective Effects on Angiotensin II-Induced Cardiomyocyte Hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Xu,Dongchen Liu,Xinyu Gao,Ziwen Wang,Linna Zhang,Hao Fan

iScience 25:104952 PubMed36065190

2022

GINS1 promotes the proliferation and migration of glioma cells through USP15-mediated deubiquitination of TOP2A.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Yang,Xiaocen Liu,Xiaolong Zhu,Mengying Zhang,Yingying Wang,Mingzhe Ma,Kun Lv

Virchows Archiv : an international journal of path 477:279-289 PubMed32086588

2020

The expression and prognostic role of EBP1 and relationship with AR in HER2+ breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Liu,Cong Xu,Danni Xu,Lu Cao,Huiqin Xue,Qingxiang Meng,Yun Niu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EBP1 antibody [EPR14569(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBP1 antibody [EPR14569(B)] (AB180602)

Flow Cytometry (Intracellular) - Anti-EBP1 antibody [EPR14569(B)] (AB180602)

Immunocytochemistry/ Immunofluorescence - Anti-EBP1 antibody [EPR14569(B)] (AB180602)

Western blot - Anti-EBP1 antibody [EPR14569(B)] (AB180602)

Western blot - Anti-EBP1 antibody [EPR14569(B)] (AB180602)

Related conjugates and formulations (10)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise