AB3452

Anti-EBP50/NHERF-1 antibody

Name: Anti-EBP50/NHERF-1 antibody (ab3452) | Abcam
Brand: Abcam Limited
SKU: AB3452
Rating: 5 (8 reviews)

(8 Reviews)

(44 Publications)

Rabbit Polyclonal EBP50/NHERF-1 antibody. Suitable for ICC, IP, WB, IHC-P, IHC-Fr and reacts with Human, Mouse, Rat, Dog samples. Cited in 44 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NHERF1 aa 250-350.

View Alternative Names

NHERF, SLC9A3R1, NHERF1, Na(+)/H(+) exchange regulatory cofactor NHE-RF1, NHERF-1, Ezrin-radixin-moesin-binding phosphoprotein 50, Regulatory cofactor of Na(+)/H(+) exchanger, Sodium-hydrogen exchanger regulatory factor 1, Solute carrier family 9 isoform A3 regulatory factor 1, EBP50

7 Images

Supplier Data

Immunocytochemistry - Anti-EBP50/NHERF-1 antibody (AB3452)

Immunocytochemistry analysis of 4% paraformaldehyde-fixed 0.1% Triton™ X-100 permeabilized T-47D cells staining EBP50/NHERF-1 with ab3452 at 2μg/ml, and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution (green). Nuclear counterstain was SlowFade® Gold Antifade Mountant with DAPI (blue), and F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin (red). Negative control used no primary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBP50/NHERF-1 antibody (AB3452)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBP50/NHERF-1 antibody (AB3452)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse airway tissue sections labeling EBP50/NHERF-1 with ab3452.

AbReview12505****

Western blot - Anti-EBP50/NHERF-1 antibody (AB3452)

All lanes:

Western blot - Anti-EBP50/NHERF-1 antibody (ab3452) at 1/1000 dilution

All lanes:

Whole cell lysate prepared from human HepG2 cells

Secondary

All lanes:

HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution

Predicted band size: 38 kDa

true

Exposure time: 40s

This image is courtesy of an anonymous abreview.

Unknown

Western blot - Anti-EBP50/NHERF-1 antibody (AB3452)

Western blot detection of recombinant Human EBP50/NHERF-1 using ab3452.

All lanes:

Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)

Predicted band size: 38 kDa

false

Supplier Data

Western blot - Anti-EBP50/NHERF-1 antibody (AB3452)

All lanes:

Western blot - Anti-EBP50/NHERF-1 antibody (ab3452) at 1 µg/mL

Lane 1:

T-47D (Human ductal breast epithelial tumor cell line) whole cell extract

Lane 2:

MCF7 (human breast adenocarcinoma cell line) whole cell extract

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

Lane 4:

Caco-2 (Human colorectal adenocarcinoma cell line) whole cell extract

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/2500 dilution

Predicted band size: 38 kDa

false

AbReview12506****

Western blot - Anti-EBP50/NHERF-1 antibody (AB3452)

All lanes:

Western blot - Anti-EBP50/NHERF-1 antibody (ab3452) at 1/1000 dilution

All lanes:

Whole tissue lysate prepared from mouse liver

Secondary

All lanes:

HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution

Predicted band size: 38 kDa

true

Exposure time: 30s

This image is courtesy of an anonymous abreview.

Supplier Data

Western blot - Anti-EBP50/NHERF-1 antibody (AB3452)

All lanes:

Western blot - Anti-EBP50/NHERF-1 antibody (ab3452) at 0.2 µg/mL

Lane 1:

Membrane enriched extracts of MCF7 (human breast adenocarcinoma cell line) at 30 µg

Lane 2:

Membrane enriched extracts of T-47D (Human ductal breast epithelial tumor cell line) at 30 µg

Lane 3:

Membrane enriched extracts of SK-BR-3 (Human mammary gland adenocarcinoma cell line) at 30 µg

Lane 4:

Membrane enriched extracts of MDA-MB-231 (human breast adenocarcinoma cell line) at 30 µg

Lane 5:

Membrane enriched extracts of HeLa (human epithelial cell line from cervix adenocarcinoma) at 30 µg

Lane 6:

Membrane enriched extracts of HepG2 (human liver hepatocellular carcinoma cell line) at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution

Predicted band size: 38 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Dog, Rat

Applications

IHC-P, WB, IHC-Fr, ICC, IP

applications

Immunogen

Recombinant Fragment Protein within Human NHERF1 aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

O14745

Specificity

Detects EBP50/NHERF-1 from human T84 and Calu3 cells as well as recombinant human protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2 µg/mL", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cow": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cynomolgus monkey": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Dog": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rabbit": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EBP50 also known as NHERF-1 (Na+/H+ exchanger regulatory factor 1) is a scaffolding protein with a mass of approximately 50 kDa. This protein plays a critical role in the formation of multiprotein complexes by its ability to bind to several different proteins simultaneously. EBP50 localizes primarily to the apical surface of epithelial cells where it is found in organs such as the kidney liver and intestines. It consists of two PDZ domains and an ezrin-binding domain which enable it to interact with various membrane proteins cytoskeletal elements and signaling molecules.

Biological function summary

EBP50 regulates cellular functions by organizing signaling complexes at the plasma membrane. It is involved in processes such as ion transport signal transduction and cell proliferation. EBP50 often acts as part of a multiprotein complex contributing to the stabilization and regulation of membrane receptors and channels. By regulating these components EBP50 affects a variety of cellular responses to external stimuli.

Pathways

EBP50 plays important roles in cellular signaling pathways like the PTH (parathyroid hormone) receptor signaling and CFTR (cystic fibrosis transmembrane conductance regulator) channel regulation. It interacts with proteins such as ezrin and PLCβ (phospholipase C beta) which facilitates the signaling cascades critical for maintaining proper cell function. By modulating these pathways EBP50 has a significant impact on maintaining cellular homeostasis and responding to changing physiological conditions.

EBP50 has been linked to conditions such as cystic fibrosis and certain cancers. With cystic fibrosis its interaction with CFTR is essential for the proper regulation of chloride channels and subsequently fluid balance in epithelial tissues. In cancer altered expression or mutations in EBP50 can affect pathways related to cell growth and metastasis highlighting interactions with the ezrin protein which couples signals from the cell membrane to actin cytoskeleton dynamics impacting tumor progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Involved in the regulation of phosphate reabsorption in the renal proximal tubules. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa.

See full target information NHERF1

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Publications (44)

Recent publications for all applications. Explore the full list and refine your search

Gut microbes 15:2225841 PubMed37350393

2023

Increased intestinal permeability and downregulation of absorptive ion transporters , , and contribute to diarrhea during infection.

Applications

Unspecified application

Species

Unspecified reactive species

F Christopher Peritore-Galve,Izumi Kaji,Anna Smith,Lauren M Walker,John A Shupe,M Kay Washington,Holly M Scott Algood,Pradeep K Dudeja,James R Goldenring,D Borden Lacy

The Journal of biological chemistry 298:101836 PubMed35307350

2022

RGS14 regulates PTH- and FGF23-sensitive NPT2A-mediated renal phosphate uptake via binding to the NHERF1 scaffolding protein.

Applications

Unspecified application

Species

Unspecified reactive species

Peter A Friedman,W Bruce Sneddon,Tatyana Mamonova,Carolina Montanez-Miranda,Suneela Ramineni,Nicholas H Harbin,Katherine E Squires,Julia V Gefter,Clara E Magyar,David R Emlet,John R Hepler

Aging 13:22588-22610 PubMed34584017

2021

GPR43 regulation of mitochondrial damage to alleviate inflammatory reaction in sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Zhang,Wusan Wang,Maodi Xu,Haitang Xie,Zhichen Pu

Journal of immunology (Baltimore, Md. : 1950) 206:2301-2311 PubMed33952618

2021

Na/H Exchanger Regulatory Factor 1 Mediates the Pathogenesis of Airway Inflammation in a Murine Model of House Dust Mite-Induced Asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Ananth K Kammala,Devika Bahal,Canchai Yang,Reynold A Panettieri,Rupali Das,Hariharan Subramanian

International journal of molecular sciences 21: PubMed33092043

2020

Sodium Hydrogen Exchanger Regulatory Factor-1 (NHERF1) Regulates Fetal Membrane Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Ananth Kumar Kammala,Samantha Sheller-Miller,Enkhtuya Radnaa,Talar Kechichian,Hariharan Subramanian,Ramkumar Menon

Journal of surgical oncology 121:547-560 PubMed31867736

2019

Prognostic and therapeutic implications of NHERF1 expression and regulation in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Premila D Leiphrakpam,Audrey J Lazenby,Sanjib Chowdhury,Lynette M Smith,Michelle Mathiesen,Michael G Brattain,Jing Wang,Jennifer D Black,Chandrakanth Are

Neuro-oncology 22:550-562 PubMed31711240

2019

HDAC inhibition induces expression of scaffolding proteins critical for tumor progression in pediatric glioma: focus on EBP50 and IRSp53.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline Capdevielle,Angélique Desplat,Justine Charpentier,Francis Sagliocco,Pierre Thiebaud,Nadine Thézé,Sandrine Fédou,Katarzyna B Hooks,Romano Silvestri,Veronique Guyonnet-Duperat,Melina Petrel,Anne-Aurélie Raymond,Jean-William Dupuy,Christophe F Grosset,Martin Hagedorn

Journal of proteomics 206:103423 PubMed31255707

2019

Identification of PKCα-dependent phosphoproteins in mouse retina.

Applications

Unspecified application

Species

Unspecified reactive species

Colin M Wakeham,Phillip A Wilmarth,Jennifer M Cunliffe,John E Klimek,Gaoying Ren,Larry L David,Catherine W Morgans

Scientific reports 8:9072 PubMed29899468

2018

Circadian clock component PERIOD2 regulates diurnal expression of Na/H exchanger regulatory factor-1 and its scaffolding function.

Applications

Unspecified application

Species

Unspecified reactive species

Yuya Tsurudome,Satoru Koyanagi,Takumi Kanemitsu,Chiharu Katamune,Masayuki Oda,Yuki Kanado,Mizuki Kato,Akari Morita,Yu Tahara,Naoya Matsunaga,Shigenobu Shibata,Shigehiro Ohdo

Development (Cambridge, England) 145: PubMed29712669

2018

Proliferation-independent role of NF2 (merlin) in limiting biliary morphogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Samira Benhamouche-Trouillet,Evan O'Loughlin,Ching-Hui Liu,William Polacheck,Julien Fitamant,Mary McKee,Nabeel El-Bardeesy,Christopher S Chen,Andrea I McClatchey

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-EBP50/NHERF-1 antibody

Immunocytochemistry - Anti-EBP50/NHERF-1 antibody (AB3452)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBP50/NHERF-1 antibody (AB3452)

Western blot - Anti-EBP50/NHERF-1 antibody (AB3452)

Western blot - Anti-EBP50/NHERF-1 antibody (AB3452)

Western blot - Anti-EBP50/NHERF-1 antibody (AB3452)

Western blot - Anti-EBP50/NHERF-1 antibody (AB3452)

Western blot - Anti-EBP50/NHERF-1 antibody (AB3452)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (44)

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