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Mouse Monoclonal EBP50/NHERF-1 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SLC9A3R1.

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Images

Flow Cytometry - Anti-EBP50/NHERF-1 antibody [EBP-10] (AB9526), expandable thumbnail
  • Western blot - Anti-EBP50/NHERF-1 antibody [EBP-10] (AB9526), expandable thumbnail

Publications

Key facts

Isotype

IgG2b

Host species

Mouse

Storage buffer

pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human SLC9A3R1. Database link O14745

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWB
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1 µg for 106 Cells

Notes

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Human

Dilution info

-

Notes

Recommended dilution: 2μg/ml. Sample preparation: Lysis buffer with maltoside (1%).Application note: Non-reducing conditions with SDS-PAGE (10% separating gel).

Associated Products

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Target data

Function

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Involved in the regulation of phosphate reabsorption in the renal proximal tubules. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa.

Alternative names

Na(+)/H(+) exchange regulatory cofactor NHE-RF1, NHERF-1, Ezrin-radixin-moesin-binding phosphoprotein 50, Regulatory cofactor of Na(+)/H(+) exchanger, Sodium-hydrogen exchanger regulatory factor 1, Solute carrier family 9 isoform A3 regulatory factor 1, EBP50, NHERF, NHERF1, SLC9A3R1

Recommended products

Mouse Monoclonal EBP50/NHERF-1 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SLC9A3R1.

Alternative names

Na(+)/H(+) exchange regulatory cofactor NHE-RF1, NHERF-1, Ezrin-radixin-moesin-binding phosphoprotein 50, Regulatory cofactor of Na(+)/H(+) exchanger, Sodium-hydrogen exchanger regulatory factor 1, Solute carrier family 9 isoform A3 regulatory factor 1, EBP50, NHERF, NHERF1, SLC9A3R1

Key facts

Isotype

IgG2b

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human SLC9A3R1. Database link O14745
Clone number

EBP-10

Purification technique

Affinity purification Protein A

Specificity

Human ERM (ezrin/radixin/moesin)-binding phosphoprotein of 50 kDa (EBP50) / Na+/H+ exchange regulatory factor (NHERF-1)

Concentration
Loading...
Purification notes

Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

EBP50 also known as NHERF-1 (Na+/H+ exchanger regulatory factor 1) is a scaffolding protein with a mass of approximately 50 kDa. This protein plays a critical role in the formation of multiprotein complexes by its ability to bind to several different proteins simultaneously. EBP50 localizes primarily to the apical surface of epithelial cells where it is found in organs such as the kidney liver and intestines. It consists of two PDZ domains and an ezrin-binding domain which enable it to interact with various membrane proteins cytoskeletal elements and signaling molecules.

Biological function summary

EBP50 regulates cellular functions by organizing signaling complexes at the plasma membrane. It is involved in processes such as ion transport signal transduction and cell proliferation. EBP50 often acts as part of a multiprotein complex contributing to the stabilization and regulation of membrane receptors and channels. By regulating these components EBP50 affects a variety of cellular responses to external stimuli.

Pathways

EBP50 plays important roles in cellular signaling pathways like the PTH (parathyroid hormone) receptor signaling and CFTR (cystic fibrosis transmembrane conductance regulator) channel regulation. It interacts with proteins such as ezrin and PLCβ (phospholipase C beta) which facilitates the signaling cascades critical for maintaining proper cell function. By modulating these pathways EBP50 has a significant impact on maintaining cellular homeostasis and responding to changing physiological conditions.

Associated diseases and disorders

EBP50 has been linked to conditions such as cystic fibrosis and certain cancers. With cystic fibrosis its interaction with CFTR is essential for the proper regulation of chloride channels and subsequently fluid balance in epithelial tissues. In cancer altered expression or mutations in EBP50 can affect pathways related to cell growth and metastasis highlighting interactions with the ezrin protein which couples signals from the cell membrane to actin cytoskeleton dynamics impacting tumor progression.

Product promise

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2 product images

  • Flow Cytometry - Anti-EBP50/NHERF-1 antibody [EBP-10] (ab9526), expandable thumbnail

    Flow Cytometry - Anti-EBP50/NHERF-1 antibody [EBP-10] (ab9526)

    Overlay histogram showing HepG2 cells stained with ab9526 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9526, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (Mouse IgG2b [PLPV219] - Isotype Control ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Western blot - Anti-EBP50/NHERF-1 antibody [EBP-10] (ab9526), expandable thumbnail

    Western blot - Anti-EBP50/NHERF-1 antibody [EBP-10] (ab9526)

    All lanes: Western blot - Anti-EBP50/NHERF-1 antibody [EBP-10] (ab9526) at 2 µg/mL

    Lane 1: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Lane 2: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Lane 3: Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary

    All lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 38 kDa

    Observed band size: 48 kDa

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Product protocols

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