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AB9526

Anti-EBP50/NHERF-1 antibody [EBP-10]

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(13 Publications)

Mouse Monoclonal EBP50/NHERF-1 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NHERF1.

View Alternative Names

NHERF, SLC9A3R1, NHERF1, Na(+)/H(+) exchange regulatory cofactor NHE-RF1, NHERF-1, Ezrin-radixin-moesin-binding phosphoprotein 50, Regulatory cofactor of Na(+)/H(+) exchanger, Sodium-hydrogen exchanger regulatory factor 1, Solute carrier family 9 isoform A3 regulatory factor 1, EBP50

2 Images
Flow Cytometry - Anti-EBP50/NHERF-1 antibody [EBP-10] (AB9526)
  • Flow Cyt

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Flow Cytometry - Anti-EBP50/NHERF-1 antibody [EBP-10] (AB9526)

Overlay histogram showing HepG2 cells stained with ab9526 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9526, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Western blot - Anti-EBP50/NHERF-1 antibody [EBP-10] (AB9526)
  • WB

Unknown

Western blot - Anti-EBP50/NHERF-1 antibody [EBP-10] (AB9526)

All lanes:

Western blot - Anti-EBP50/NHERF-1 antibody [EBP-10] (ab9526) at 2 µg/mL

Lane 1:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 38 kDa

Observed band size: 48 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

EBP-10

Isotype

IgG2b

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NHERF1.

O14745

Specificity

Human ERM (ezrin/radixin/moesin)-binding phosphoprotein of 50 kDa (EBP50) / Na+/H+ exchange regulatory factor (NHERF-1)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>Recommended dilution: 2μg/ml. Sample preparation: Lysis buffer with maltoside (1%).Application note: Non-reducing conditions with SDS-PAGE (10% separating gel).</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EBP50 also known as NHERF-1 (Na+/H+ exchanger regulatory factor 1) is a scaffolding protein with a mass of approximately 50 kDa. This protein plays a critical role in the formation of multiprotein complexes by its ability to bind to several different proteins simultaneously. EBP50 localizes primarily to the apical surface of epithelial cells where it is found in organs such as the kidney liver and intestines. It consists of two PDZ domains and an ezrin-binding domain which enable it to interact with various membrane proteins cytoskeletal elements and signaling molecules.
Biological function summary

EBP50 regulates cellular functions by organizing signaling complexes at the plasma membrane. It is involved in processes such as ion transport signal transduction and cell proliferation. EBP50 often acts as part of a multiprotein complex contributing to the stabilization and regulation of membrane receptors and channels. By regulating these components EBP50 affects a variety of cellular responses to external stimuli.

Pathways

EBP50 plays important roles in cellular signaling pathways like the PTH (parathyroid hormone) receptor signaling and CFTR (cystic fibrosis transmembrane conductance regulator) channel regulation. It interacts with proteins such as ezrin and PLCβ (phospholipase C beta) which facilitates the signaling cascades critical for maintaining proper cell function. By modulating these pathways EBP50 has a significant impact on maintaining cellular homeostasis and responding to changing physiological conditions.

EBP50 has been linked to conditions such as cystic fibrosis and certain cancers. With cystic fibrosis its interaction with CFTR is essential for the proper regulation of chloride channels and subsequently fluid balance in epithelial tissues. In cancer altered expression or mutations in EBP50 can affect pathways related to cell growth and metastasis highlighting interactions with the ezrin protein which couples signals from the cell membrane to actin cytoskeleton dynamics impacting tumor progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Involved in the regulation of phosphate reabsorption in the renal proximal tubules. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa.
See full target information NHERF1
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 298:101836 PubMed35307350

2022

RGS14 regulates PTH- and FGF23-sensitive NPT2A-mediated renal phosphate uptake via binding to the NHERF1 scaffolding protein.

Applications

Unspecified application

Species

Unspecified reactive species

Peter A Friedman,W Bruce Sneddon,Tatyana Mamonova,Carolina Montanez-Miranda,Suneela Ramineni,Nicholas H Harbin,Katherine E Squires,Julia V Gefter,Clara E Magyar,David R Emlet,John R Hepler

iScience 24:102770 PubMed34189428

2021

ACE2 interaction with cytoplasmic PDZ protein enhances SARS-CoV-2 invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Qiangmin Zhang,Julia Gefter,W Bruce Sneddon,Tatyana Mamonova,Peter A Friedman

The Journal of biological chemistry 294:887-901 PubMed30463939

2018

Inhibition of ezrin causes PKCα-mediated internalization of erbb2/HER2 tyrosine kinase in breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jaekwang Jeong,Jungmin Choi,Wonnam Kim,Pamela Dann,Farzin Takyar,Julia V Gefter,Peter A Friedman,John J Wysolmerski

American journal of physiology. Renal physiology 311:F343-51 PubMed27279487

2016

The NHERF1 PDZ1 domain and IRBIT interact and mediate the activation of Na+/H+ exchanger 3 by ANG II.

Applications

Unspecified application

Species

Unspecified reactive species

Peijian He,Luqing Zhao,Yi Ran No,Serhan Karvar,C Chris Yun

The Journal of clinical investigation 125:3519-31 PubMed26258413

2015

Restoration of Na+/H+ exchanger NHE3-containing macrocomplexes ameliorates diabetes-associated fluid loss.

Applications

Unspecified application

Species

Unspecified reactive species

Peijian He,Luqing Zhao,Lixin Zhu,Edward J Weinman,Roberto De Giorgio,Michael Koval,Shanthi Srinivasan,C Chris Yun

Autophagy 11:2323-34 PubMed26218645

2015

SLC9A3R1 stimulates autophagy via BECN1 stabilization in breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Liu,Yan Ma,Hong-Wei He,Jia-Ping Wang,Jian-Dong Jiang,Rong-Guang Shao

The Journal of biological chemistry 287:43312-21 PubMed23109343

2012

Na+/H+ exchanger regulatory factor 1 (NHERF1) directly regulates osteogenesis.

Applications

WB

Species

Mouse

Li Liu,Veronica Alonso,Lida Guo,Irina Tourkova,Sarah E Henderson,Alejandro J Almarza,Peter A Friedman,Harry C Blair

The Journal of biological chemistry 287:24148-63 PubMed22628548

2012

Ezrin-anchored protein kinase A coordinates phosphorylation-dependent disassembly of a NHERF1 ternary complex to regulate hormone-sensitive phosphate transport.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Wang,Chris K Means,Yanmei Yang,Tatyana Mamonova,Alessandro Bisello,Daniel L Altschuler,John D Scott,Peter A Friedman

American journal of physiology. Renal physiology 296:F1118-28 PubMed19225049

2009

GABARAP deficiency modulates expression of NaPi-IIa in renal brush-border membranes.

Applications

Unspecified application

Species

Unspecified reactive species

Sonja C Reining,Serge M Gisler,Daniel Fuster,Orson W Moe,Gregory A O'Sullivan,Heinrich Betz,Jürg Biber,Heini Murer,Nati Hernando

Developmental cell 6:855-64 PubMed15177033

2004

Ezrin is essential for epithelial organization and villus morphogenesis in the developing intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Ichiko Saotome,Marcello Curto,Andrea I McClatchey
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

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