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Rabbit Polyclonal EBS antibody. N-terminal. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human EBS aa 1-100.

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Images

Western blot - Anti-EBS antibody - N-terminal (AB224439), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBS antibody - N-terminal (AB224439), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human EBS aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BTE0

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/20.00000 - 1/50.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Target data

Function

N-acetyltransferase that mediates the acetylation of the N-terminal residues of alpha- and beta-tubulin.

Alternative names

Recommended products

Rabbit Polyclonal EBS antibody. N-terminal. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human EBS aa 1-100.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human EBS aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BTE0
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

EBS also known as Epidermal Basement Scaffold or EBS1 is a structural protein involved in the assembly and maintenance of extracellular matrices. EBS has a molecular mass of about 180 kDa. You will find it expressed abundantly in skin tissue particularly within the basal layer of the epidermis where it connects to the basement membrane. The protein interacts with other key structural proteins to maintain tissue integrity providing support and mechanical strength to the skin.

Biological function summary

EBS plays a role in anchoring the dermal and epidermal layers of the skin. It forms part of a complex with proteins like collagen IV and laminin which contribute to the dermal-epidermal junction’s architecture and function. This complex helps facilitate cell adhesion and migration essential processes in wound healing and tissue repair. EBS’s interactions with these structural proteins fine-tune cellular environments and impact cell signaling.

Pathways

EBS influences the integrin signaling and extracellular matrix remodeling pathways. In integrin signaling EBS supports cellular adhesion by interacting with integrin receptors that mediate the communication between cells and the extracellular matrix. This interaction also influences cytoskeleton reorganization via proteins such as talin and vinculin. In terms of extracellular matrix remodeling EBS has relationships with matrix metalloproteinases (MMPs) that regulate tissue remodeling and degradation affecting tissue dynamics and morphogenesis.

Associated diseases and disorders

Malfunction or alterations in EBS expression contribute to skin disorders such as epidermolysis bullosa and psoriasis. Epidermolysis bullosa involves a deficiency in EBS leading to fragile skin that easily blisters. EBS’s connection with collagen XVII further complicates the condition impacting dermal-epidermal cohesion. In psoriasis a chronic inflammatory skin condition dysregulated expression of EBS and MMPs affects skin turnover and inflammation resulting in plaques and scaling on the skin surface.

Product promise

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2 product images

  • Western blot - Anti-EBS antibody - N-terminal (ab224439), expandable thumbnail

    Western blot - Anti-EBS antibody - N-terminal (ab224439)

    All lanes: Western blot - Anti-EBS antibody - N-terminal (ab224439) at 0.4 µg/mL

    Lane 1: Control (vector only transfected) - HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Lane 2: EBS over-expression - HEK-293T whole cell lysate, Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)

    Predicted band size: 23 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBS antibody - N-terminal (ab224439), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBS antibody - N-terminal (ab224439)

    Immunohistochemical analysis of human spleen tissue labeling EBS in the cytoplasm in subsets of cells in the red pulp with ab224439 at 1/20 dilution.

    Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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