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AB224439

Anti-EBS antibody - N-terminal

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(1 Publication)

Rabbit Polyclonal EBS antibody. N-terminal. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NAT9 aa 1-100.

View Alternative Names

EBS, NAT9, Alpha/beta-tubulin-N-acetyltransferase 9, Embryo brain-specific protein

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBS antibody - N-terminal (AB224439)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBS antibody - N-terminal (AB224439)

Immunohistochemical analysis of human spleen tissue labeling EBS in the cytoplasm in subsets of cells in the red pulp with ab224439 at 1/20 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-EBS antibody - N-terminal (AB224439)
  • WB

Supplier Data

Western blot - Anti-EBS antibody - N-terminal (AB224439)

All lanes:

Western blot - Anti-EBS antibody - N-terminal (ab224439) at 0.4 µg/mL

Lane 1:

Control (vector only transfected) - HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

EBS over-expression - HEK-293T whole cell lysate, Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NAT9 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9BTE0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EBS also known as Epidermal Basement Scaffold or EBS1 is a structural protein involved in the assembly and maintenance of extracellular matrices. EBS has a molecular mass of about 180 kDa. You will find it expressed abundantly in skin tissue particularly within the basal layer of the epidermis where it connects to the basement membrane. The protein interacts with other key structural proteins to maintain tissue integrity providing support and mechanical strength to the skin.
Biological function summary

EBS plays a role in anchoring the dermal and epidermal layers of the skin. It forms part of a complex with proteins like collagen IV and laminin which contribute to the dermal-epidermal junction’s architecture and function. This complex helps facilitate cell adhesion and migration essential processes in wound healing and tissue repair. EBS’s interactions with these structural proteins fine-tune cellular environments and impact cell signaling.

Pathways

EBS influences the integrin signaling and extracellular matrix remodeling pathways. In integrin signaling EBS supports cellular adhesion by interacting with integrin receptors that mediate the communication between cells and the extracellular matrix. This interaction also influences cytoskeleton reorganization via proteins such as talin and vinculin. In terms of extracellular matrix remodeling EBS has relationships with matrix metalloproteinases (MMPs) that regulate tissue remodeling and degradation affecting tissue dynamics and morphogenesis.

Malfunction or alterations in EBS expression contribute to skin disorders such as epidermolysis bullosa and psoriasis. Epidermolysis bullosa involves a deficiency in EBS leading to fragile skin that easily blisters. EBS’s connection with collagen XVII further complicates the condition impacting dermal-epidermal cohesion. In psoriasis a chronic inflammatory skin condition dysregulated expression of EBS and MMPs affects skin turnover and inflammation resulting in plaques and scaling on the skin surface.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

N-acetyltransferase that mediates the acetylation of the N-terminal residues of alpha- and beta-tubulin.
See full target information NAT9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:20430 PubMed37993584

2023

NPLOC4 is a potential target and a poor prognostic signature in lung squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Naixue Wang,Dantong Zhu,Yao Liu,Jingran Wu,Meiling Wang,Shanxiu Jin,Fangwei Fu,Baolei Li,Hongjuan Ji,Cheng Du,Zhendong Zheng
View all publications
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Product promise

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