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AB150625

Anti-EBV Induced Gene 2/EBI2 antibody

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(2 Publications)

Rabbit Polyclonal EBV Induced Gene 2/EBI2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

EBI2, GPR183, G-protein coupled receptor 183, Epstein-Barr virus-induced G-protein coupled receptor 2, EBV-induced G-protein coupled receptor 2, hEBI2

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBV Induced Gene 2/EBI2 antibody (AB150625)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBV Induced Gene 2/EBI2 antibody (AB150625)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lymph node tissue labeling EBV Induced Gene 2/EBI2 with ab150625 at 4 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

BLAST analysis of the peptide immunogen showed no homology with other Human proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "4 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Common marmoset": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Elephant": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Opossum": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Platypus": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1 month
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store undiluted
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EBV Induced Gene 2 (EBI2) also known as GPR183 is a G protein-coupled receptor with a mass of approximately 41 kDa. It is prominently expressed in immune cells such as B cells and T cells. EBI2 is activated by oxysterols which are oxidized derivatives of cholesterol and triggers a signaling cascade that influences cell positioning and migration. The receptor plays an important role in guiding B cell migration during immune responses especially within lymphoid tissues.
Biological function summary

EBI2 supports the positioning of immune cells within secondary lymphoid organs by detecting specific chemokine gradients. It does not form part of larger complexes but instead functions independently to interpret chemotactic signals. EBI2 is important during B cell maturation orchestrating the cellular movement that is essential for executing adaptive immune responses effectively.

Pathways

EBI2 integrates into the oxysterol signaling pathway which directly impacts immune cell dynamics. Through its effects on B cell migration its function connects to the chemokine signaling pathway. EBI2 interacts with proteins like CXCR5 enabling the directed movement of B cells towards follicular dendritic cells playing a role in ensuring effective humoral immunity.

Irregular EBI2 expression correlates with autoimmune conditions such as rheumatoid arthritis and lupus. Aberrant signaling through EBI2 can contribute to improper immune cell localization which is a characteristic of these disorders. The receptor's interplay with CXCR5 is particularly significant in the context of rheumatoid arthritis as both are implicated in the dysregulation of immune cell homing leading to inflammation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

G-protein coupled receptor expressed in lymphocytes that acts as a chemotactic receptor for B-cells, T-cells, splenic dendritic cells, monocytes/macrophages and astrocytes (By similarity). Receptor for oxysterol 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) and other related oxysterols (PubMed : 21796212, PubMed : 22875855, PubMed : 22930711). Mediates cell positioning and movement of a number of cells by binding the 7-alpha,25-OHC ligand that forms a chemotactic gradient (By similarity). Binding of 7-alpha,25-OHC mediates the correct localization of B-cells during humoral immune responses (By similarity). Guides B-cell movement along the B-cell zone-T-cell zone boundary and later to interfollicular and outer follicular regions (By similarity). Its specific expression during B-cell maturation helps position B-cells appropriately for mounting T-dependent antibody responses (By similarity). Collaborates with CXCR5 to mediate B-cell migration; probably by forming a heterodimer with CXCR5 that affects the interaction between of CXCL13 and CXCR5 (PubMed : 22913878). Also acts as a chemotactic receptor for some T-cells upon binding to 7-alpha,25-OHC ligand (By similarity). Promotes follicular helper T (Tfh) cells differentiation by positioning activated T-cells at the follicle-T-zone interface, promoting contact of newly activated CD4 T-cells with activated dendritic cells and exposing them to Tfh-cell-promoting inducible costimulator (ICOS) ligand (By similarity). Expression in splenic dendritic cells is required for their homeostasis, localization and ability to induce B- and T-cell responses : GPR183 acts as a chemotactic receptor in dendritic cells that mediates the accumulation of CD4(+) dendritic cells in bridging channels (By similarity). Regulates migration of astrocytes and is involved in communication between astrocytes and macrophages (PubMed : 25297897). Promotes osteoclast precursor migration to bone surfaces (By similarity). Signals constitutively through G(i)-alpha, but not G(s)-alpha or G(q)-alpha (PubMed : 21673108, PubMed : 25297897). Signals constitutively also via MAPK1/3 (ERK1/2) (By similarity).
See full target information GPR183
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Annals of the rheumatic diseases 83:926-944 PubMed38325908

2024

Unveiling inflammatory and prehypertrophic cell populations as key contributors to knee cartilage degeneration in osteoarthritis using multi-omics data integration.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Fan,Xuzhao Bian,Xiaogao Meng,Lei Li,Laiyi Fu,Yanan Zhang,Long Wang,Yan Zhang,Dalong Gao,Xiong Guo,Mikko Juhani Lammi,Guangdun Peng,Shiquan Sun

Experimental biology and medicine (Maywood, N.J.) 247:700-711 PubMed35044873

2022

Specialized pro-resolution mediators in the bladder: Receptor expression and recovery of bladder function from cystitis.

Applications

Unspecified application

Species

Unspecified reactive species

Francis M Hughes,Armand Allkanjari,Michael R Odom,Huixia Jin,J Todd Purves
View all publications
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