JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB316860

Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78]

  • BOND RX™ Validated
  • 20ul selling size
  • Recombinant
  • RabMAb
  • What is this?

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Recombinant Monoclonal EBV Nuclear Antigen/EBNA1 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), I-ELISA and reacts with Transfected cell lysate - Epstein-Barr Virus (Strain B95-8), Transfected cell line - Epstein-Barr Virus (Strain B95-8), Synthetic peptide - Epstein-Barr Virus (Strain B95-8) samples. Cited in 2 publications.

View Alternative Names

BKRF1, EBNA1, Epstein-Barr nuclear antigen 1, EBNA-1, EBV nuclear antigen 1

8 Images
Immunocytochemistry/ Immunofluorescence - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] (AB316860)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] (AB316860)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) transfected with an EBV Nuclear Antigen/EBNA1 expression vector containing a myc-His-tag®. cells labelling EBV Nuclear Antigen/EBNA1 with ab316860 at 1/4000 (0.13 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing positive staining in 293T cells transfected with an EBV Nuclear Antigen/EBNA1 expression vector containing a myc-His-tag®.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain at 1/100 (5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] (AB316860)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] (AB316860)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling EBV Nuclear Antigen/EBNA1 with ab316860 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human kidney.
The section was incubated with ab316860 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] (AB316860)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] (AB316860)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with an EBV Nuclear Antigen/EBNA1 expression vector containing a myc-His-tag®. (B) HEK-293T transfected with an empty vector containing a myc-His-tag®. tissue labeling EBV Nuclear Antigen/EBNA1 with ab316860 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T transfected with an EBV Nuclear Antigen/EBNA1 expression vector containing a myc-His-tag®. No staining on (B) HEK-293T transfected with an empty vector containing a myc-His-tag®.
The section was incubated with ab316860 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Flow Cytometry (Intracellular) - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] (AB316860)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] (AB316860)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T cells transfected with an EBV Nuclear Antigen/EBNA1 expression vector containing a myc-His-tag® (Middle) / 293T cells transfected with an empty expression vector containing a myc-His-tag® (Right) cells labelling EBV Nuclear Antigen/EBNA1 with ab316860 at 1/5000 dilution (0.01 ug)/Middle and Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells are co-stained with Myc tag conjugated to Alexa Fluor®647.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] (AB316860)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] (AB316860)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling EBV Nuclear Antigen/EBNA1 with ab316860 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse kidney.
The section was incubated with ab316860 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] (AB316860)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] (AB316860)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling EBV Nuclear Antigen/EBNA1 with ab316860 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on rat kidney.
The section was incubated with ab316860 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Indirect ELISA - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] (AB316860)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] (AB316860)

Indirect ELISA analysis of ab316860 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : EBV Nuclear Antigen/EBNA1.

Antigen concentration : 1000 ng/ml

Western blot - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] (AB316860)
  • WB

Supplier Data

Western blot - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] (AB316860)

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

In Western blot, Anti-6X His tag antibody [EPR20547]-CHIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] (ab316860) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with an EBV Nuclear Antigen/EBNA1 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 20-70 kDa,124 kDa

false

Exposure time: 3s

  • Carrier free

    Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28706-78

Isotype

IgG

Carrier free

No

Reacts with

Epstein-Barr Virus (Strain B95-8)

Applications

ICC/IF, I-ELISA, WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB78113

Anti-EBV Latent Membrane Protein 1 antibody [CS 1-4]

  • 4
  • 4
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB313343

Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] (AB313343)

  • 5
  • 1
Proteins & Peptides

AB138345

Recombinant EBV Nuclear Antigen/EBNA1 protein

  • 5
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Epstein-Barr Virus (Strain B95-8)": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Synthetic peptide - Epstein-Barr Virus (Strain B95-8)": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "1 µg/mL", "IELISA-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Transfected cell line - Epstein-Barr Virus (Strain B95-8)": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/4000", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Transfected cell lysate - Epstein-Barr Virus (Strain B95-8)": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EBV Nuclear Antigen 1 (EBNA1) is a protein expressed by the Epstein-Barr Virus (EBV) also known as Human Herpesvirus 4. It has a molecular weight of approximately 80 kDa and shows expression in latently infected cells. EBNA1 acts as a viral episome-maintenance protein that binds to the EBV genome ensuring viral DNA replication and partitioning during cell division. This protein facilitates the persistent infection of EBV in host cells. Occasionally it is also referred to as EBV EBNA or EBNA-1 protein.
Biological function summary

EBNA1 functions as an EBV-specific DNA-binding protein essential for maintaining the viral episome within infected cells. EBNA1 plays a role in gene regulation by interacting with specific DNA sequences called oriP to assist in the replication and maintenance of the viral DNA. It does not form part of a larger protein complex but strongly influences cellular processes through these interactions. EBNA1 ensures the survival and propagation of the virus without immediate cytopathic effects contributing to its long-term persistence.

Pathways

EBNA1 is central to viral latency and replication pathways in host cells. EBNA1 influences both the EBV lytic and latent cycles maintaining the latency by stabilizing the viral episome within the nucleus of host cells and regulating viral gene expression. It interacts with cellular proteins like USP7 a deubiquitinase important for the regulation of EBNA1 stability and function. These pathways highlight EBNA1's role in the balance between viral latency and active replication.

EBNA1 is closely associated with several EBV-related cancers such as Burkitt's lymphoma and nasopharyngeal carcinoma. The persistent expression of EBNA1 contributes to oncogenic processes by disrupting normal cellular control mechanisms. EBNA1's interaction with p53 a tumor suppressor protein can interfere with the host's cell-cycle regulation and apoptosis allowing for malignant transformation. These interactions illustrate EBNA1's significant role in the pathogenesis of disorders linked to EBV infection.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Responsible for the origin of replication (oriP) dependent replication and maintenance of viral episomes during latent infection (PubMed : 15479791, PubMed : 2996781). EBNA1 dimer interacts with the DS (dyad symmetry) element within the origin of replication oriP and with a host mitotic chromosome to initiate viral DNA replication during latency (PubMed : 24067969, PubMed : 2996781, PubMed : 31142669, PubMed : 8551585). EBNA1 binding to DS recruits the host origin recognition complex (ORC) (PubMed : 12953058). Governs the faithful mitotic segregation of the viral episomes by binding both the FR (family of repeats) element within oriP and the host mitotic chromosomes (PubMed : 11172042, PubMed : 15479791, PubMed : 24067969). Forms a cell cycle-dependent tyrosine-dependent DNA cross-link and single-strand cleavage at oriP required for terminating replication and maintaining viral episomes (PubMed : 33482082). Counteracts the stabilization of host p53/TP53 by host USP7, thereby decreasing apoptosis and increasing host cell survival (PubMed : 15808506). Induces degradation of host PML through the ubiquitin-proteasome system, which promotes lytic reactivation and may impair the host cell DNA repair (PubMed : 18833293). Increases the association of CK2 with PML proteins which increases the phosphorylation of PML proteins by CK2, triggering the polyubiquitylation and degradation of PML (PubMed : 20719947). Displays inhibitory effects on a SUMO2-modified complex that includes STUB1, KAP1 and USP7 (PubMed : 32176739). This inhibitory effect possibly participates to the maintenance of latency linked to PML silencing (PubMed : 32176739).
See full target information EBNA1
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncogene 44:3609-3624 PubMed40764753

2025

MUC1-C auto-regulatory complex with EBNA1 is responsible for latent Epstein-Barr virus-associated gastric cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroki Ozawa,Yin Wang,Henry G Withers,Naoki Haratake,Ayako Nakashoji,Atrayee Bhattacharya,Atsushi Fushimi,Chie Kikutake,Kazuhiro Yamanoi,Shaowen White,Keyi Wang,Tatsuaki Daimon,Keisuke Shigeta,Kazumasa Fukuda,Hirofumi Kawakubo,Yuko Kitagawa,Mark D Long,Benjamin E Gewurz,Donald Kufe

MedComm 5:e739 PubMed39399647

2024

Structure-based design of antibodies targeting the EBNA1 DNA-binding domain to block Epstein-Barr virus latent infection and tumor growth.

Applications

Unspecified application

Species

Unspecified reactive species

Yongyue Han,Fang Wu,Ying Zhang,Jun Liu,Yuzhe Wu,Yuecheng Wang,Xiwen Jiang,Xin Chen,Wei Xu
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com