Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] - BSA and Azide free (AB316861)

This data was developed using ab316860, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling EBV Nuclear Antigen/EBNA1 with ab316860 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human kidney.
The section was incubated with ab316860 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] - BSA and Azide free (AB316861)

This data was developed using ab316860, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) transfected with an EBV Nuclear Antigen/EBNA1 expression vector containing a myc-His-tag®. cells labelling EBV Nuclear Antigen/EBNA1 with ab316860 at 1/4000 (0.13 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing positive staining in 293T cells transfected with an EBV Nuclear Antigen/EBNA1 expression vector containing a myc-His-tag®.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain at 1/100 (5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] - BSA and Azide free (AB316861)

This data was developed using ab316860, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T cells transfected with an EBV Nuclear Antigen/EBNA1 expression vector containing a myc-His-tag® (Middle) / 293T cells transfected with an empty expression vector containing a myc-His-tag® (Right) cells labelling EBV Nuclear Antigen/EBNA1 with ab316860 at 1/5000 dilution (0.01 ug)/Middle and Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells are co-stained with Myc tag conjugated to Alexa Fluor®647.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] - BSA and Azide free (AB316861)

This data was developed using ab316860, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with an EBV Nuclear Antigen/EBNA1 expression vector containing a myc-His-tag®. (B) HEK-293T transfected with an empty vector containing a myc-His-tag®. tissue labeling EBV Nuclear Antigen/EBNA1 with ab316860 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T transfected with an EBV Nuclear Antigen/EBNA1 expression vector containing a myc-His-tag®. No staining on (B) HEK-293T transfected with an empty vector containing a myc-His-tag®.
The section was incubated with ab316860 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] - BSA and Azide free (AB316861)

This data was developed using ab316860, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling EBV Nuclear Antigen/EBNA1 with ab316860 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on rat kidney.
The section was incubated with ab316860 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] - BSA and Azide free (AB316861)

This data was developed using ab316860, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling EBV Nuclear Antigen/EBNA1 with ab316860 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse kidney.
The section was incubated with ab316860 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• I-ELISA

Supplier Data

Indirect ELISA - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] - BSA and Azide free (AB316861)

This data was developed using ab316860, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab316860 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : EBV Nuclear Antigen/EBNA1.

Antigen concentration : 1000 ng/ml

• WB

Supplier Data

Western blot - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] - BSA and Azide free (AB316861)

This data was developed using ab316860, the same antibody clone in a different buffer formulation.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

In Western blot, Anti-6X His tag antibody [EPR20547]-CHIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-EBV Nuclear Antigen/EBNA1 antibody [EPR28706-78] (<a href='/en-us/products/primary-antibodies/ebv-nuclear-antigen-ebna1-antibody-epr28706-78-ab316860'>ab316860</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 2:

293T cells transfected with an EBV Nuclear Antigen/EBNA1 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 20-70 kDa,124 kDa

false

Exposure time: 3s